The analyses of photoreceptor regeneration from cilio-retinal stem cells using flatmount technique

• Project/Area Number: 19791263
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Nagoya University
• Principal Investigator: NISHIGUCHI Koji Nagoya University, 医学部附属病院, 助教 (30447825)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,650,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 網膜変性 / 毛様体上皮 / 網膜 / 視細胞 / 網膜発生

Research Abstract

遺伝性網膜変性マウスと薬剤性の後天性網膜変性モデルを用いて毛様体上皮組織における網膜神経細胞の再生についてin vivoで検討した。
その結果、成体の哺乳類の毛様体は、網膜障害に反応して網膜神経細胞を分化・再生する役割を担っていると考えられた。
そのメカニズムをさらに解明することにより、多くの神経細胞を失った重症な網膜変性患者に対する、網膜神経再生治療への新たな道が開かれる潜在性を秘めている。

Research Products

• [Journal Article] Generation of immature retinal neurons from proliferating cells in the pars plana after retinal histogenesis in mice with retinal degeneration (2009)
  • Author(s): Nishiguchi KM, Kaneko H, Nakamura M, Kachi S, Terasaki H
  • Journal Title: Mol Vis 15 Pages: 187-199
  • Peer Reviewed
• [Journal Article] Generation of immature retinal neurons from proliferating cells in the pars plana after retinal histogenesis in mice with retinal degeneration (2009)
  • Author(s): Nishiguchi KM
  • Journal Title: Mol Vis 15 Pages: 187-199
  • Peer Reviewed
• [Journal Article] Characteristics of Bone Marrow-Derived Microglia in the Normal and Injured Retina (2008)
  • Author(s): Kaneko H, Nishiguchi KM, Nakamura M, Kachi S, Terasaki H
  • Journal Title: Invest Ophthalmol Vis Sci 49(9) Pages: 4162-4168
  • Peer Reviewed
• [Journal Article] Retardation of Photoreceptor Degeneration in the Detached Retina of rd1 Mouse (2008)
  • Author(s): Kaneko H, Nishiguchi KM, Nakamura M, Kachi S, Terasaki H
  • Journal Title: Invest Ophthalmol Vis Sci 49(2) Pages: 781-787
  • Peer Reviewed
• [Journal Article] Identification of photoreceptor precursors in the pars plana during ocular development and after retinal injury (2008)
  • Author(s): Nishiguchi KM, Kaneko H, Nakamura M, Kachi S, Terasaki H
  • Journal Title: Invest Ophthalmol Vis Sci 49(1) Pages: 422-428
  • Peer Reviewed
• [Journal Article] Characteristics of Bone Marrow-Derived Microglia in the Normal and Injured Retina (2008)
  • Author(s): Kaneko H
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 4162-4168
  • Peer Reviewed
• [Journal Article] VEGF Stimulates Proliferation of Retinal Progenitor Cells via VEGFR2/Flk1 in Murine Retinal Degeneration (2007)
  • Author(s): Nishiguchi KM
  • Journal Title: Invest Ophthalmol Vis Sci 48 Pages: 4315-4320
  • Peer Reviewed
• [Journal Article] Retinopathy mutations in the bZIP protein NRL alter phosphorylation and transcriptional activity (2007)
  • Author(s): Kanda A,
  • Journal Title: Hum Mutat 28 Pages: 589-598
  • Peer Reviewed
• [Journal Article] Identification of photoreceptor precursors in the pars plana during ocular development and after retinal injury (2007)
  • Author(s): Nishiguchi KM
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 422-428
  • Peer Reviewed
• [Journal Article] Retardation of Photoreceptor Degeneration in the Detached Retina of rd1 Mouse (2007)
  • Author(s): Kaneko H
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 781-787
  • Peer Reviewed
• [Presentation] 毛様体扁平部と網膜の発生 (2008)
  • Author(s): 西口康二
  • Place of Presentation: 愛知県、蒲郡市
  • Year and Date: 2008-08-24
• [Presentation] 毛様体扁平部と網膜の発生 (2008)
  • Author(s): Nishiguchi KM
  • Organizer: Japan Macular Club
  • Place of Presentation: 愛知県蒲郡市
  • Year and Date: 2008-08-24
• [Presentation] Circulating Bone Marrow Derived Microglias Engraft Into the Injured Retina (2008)
  • Author(s): Hiroki Kaneko, Koji M Nishiguchi, Makoto Nakamura, Shu Kachi, Hiroko Terasaki
  • Organizer: ARVO 2008
  • Place of Presentation: Ft Lauderdale, FL, USA
  • Year and Date: 2008-05-03
• [Presentation] Increased Number and Prolonged Presence of Retinal Precursors in the Pars Plana of rdl Mice (2008)
  • Author(s): Nishiguchi KM
  • Organizer: ARVO 2008
  • Place of Presentation: Ft Lauderdale, FL, USA
  • Year and Date: 2008-05-03
• [Presentation] Increased Number and Prolonged Presence of Retinal Precursors in the Pars Plana of rd1 Mice (2008)
  • Author(s): Koji M Nishiguchi, Hiroki Kaneko, Makoto Nakamura, Shu Kachi, Hiroko Terasaki
  • Organizer: 2008 ARVO
  • Place of Presentation: Ft Lauderdale, FL, USA
  • Year and Date: 2008-05-01
• [Presentation] 骨髄由来細胞のマウス網膜内への生着 (2007)
  • Author(s): 兼子裕規、西口康二、中村誠、加地秀、寺崎浩子
  • Organizer: 第46回日本網膜硝子体学会
  • Place of Presentation: 青森県、青森市
  • Year and Date: 2007-11-24
• [Presentation] 骨髄由来細胞のマウス網膜内への生着 (2007)
  • Author(s): 兼子裕規
  • Organizer: 第46回日本網膜硝子体学会
  • Place of Presentation: 青森
  • Year and Date: 2007-11-24
• [Presentation] 毛様体上皮細胞の網膜神経細胞への分科と周辺網膜の形成 (2007)
  • Author(s): 西口康二
  • Organizer: 第46回日本網膜硝子体学会
  • Place of Presentation: 青森県、青森市
  • Year and Date: 2007-11-23
• [Presentation] 毛様体上皮細胞の網膜神経細胞への分科と周辺網膜の形成 (2007)
  • Author(s): 西口康二
  • Organizer: 第46回日本網膜硝子体学会
  • Place of Presentation: 青森
  • Year and Date: 2007-11-23
• [Presentation] KCNV2遺伝子に変異が検出されたCone dystrophy with supernormal rod ERGの一例 (2007)
  • Author(s): 中村誠、安間哲宏、西口康二、伊藤逸毅、近藤峰生、寺崎浩子
  • Organizer: 第61回日本臨床眼科学会
  • Place of Presentation: 京都府、京都市
  • Year and Date: 2007-10-13
• [Presentation] KCNV2遺伝子に変異が検出されたCone dystrophy with supernormal rod ERGの一例 (2007)
  • Author(s): 中村誠
  • Organizer: 第61回日本臨床眼科学会
  • Place of Presentation: 京都
  • Year and Date: 2007-10-13