The estabIishment of well-differentiated corneal epithelial cell line and the analysis of mechanism for corneal epithelial differentiation

• Project/Area Number: 19791271
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Yamaguchi University
• Principal Investigator: KIMURA Kazuhiro Yamaguchi University, 医学部, 助教 (60335255)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 細胞組織 / シグナル伝達 / 遺伝子 / 細胞・組織

Research Abstract

角膜上皮は、角膜表層でバリアーとして重要な働きをしている。これまで継代可能な角膜上皮細胞には正常なGap Junction(GJ)が存在せず、細胞-細胞間での分子の交換がなされていない。つまり、統合的な分化形質を保持した培養角膜上皮細胞は、未だ存在しないことを示唆している。そこで、GJ構成分子の一つであるコネキシン43に着目し、正常なGJ機能を有し,角膜上皮分子マーカーの発現を維持した Human コネキシン43安定発現角膜上皮細胞株の樹立に成功した。

Research Products

• [Journal Article] Role of Formation of an ERK-FAK-Paxillin Complex in Migration of Human Corneal Epithelial Cells During Wound Closure in Vitro. (2009)
  • Author(s): Teranishi S, Kimura K, Nishida T
  • Journal Title: Investigative Ophthalmlogoy and Visual Science (in press)
• [Journal Article] Interleukin-1 β-Induced Disruption of Barrier Function in Cultured Human Corneal Epithelial Cells. (2009)
  • Author(s): Kimura K, Teranishi S, Nishida T
  • Journal Title: Investigative Ophthalmology and Visual Science 50(2) Pages: 597-603
• [Journal Article] Interleukin-1 β-Induced Disruption of Barrier Function in Cultured Human Corneal Epithelial Cells (2009)
  • Author(s): Kazuhiro Kimura
  • Journal Title: Investigative Ophthalmology & Visual Science 50 Pages: 597-603
  • Peer Reviewed
• [Journal Article] Cytokine, Chemokine, and Adhesion Molecule Expression Mediated by Mitogen-Activated Protein Kinases in Human Corneal Fibroblasts Exposed to Poly(I:C). (2008)
  • Author(s): Liu Y, Kimura K
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 3336-3344
  • Peer Reviewed
• [Journal Article] Protection of human corneal epithelial cells from hypoxia-induced disruption of barrier function by keratinocyte growth factor. (2008)
  • Author(s): Teranishi S, Kimura K (その他2名,2番目)
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 2432-2437
  • Peer Reviewed
• [Journal Article] Induction by Latanoprost of Collagen Gel Contraction Mediated by Human Tenon Fibroblasts : Role of Intracellular Signaling Molecules. (2008)
  • Author(s): Liu Y, Ji-Ae Ko, Yanai R, Kimura K (その他3名,4番目)
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 1429-1436
  • Peer Reviewed
• [Journal Article] Delayed Disruption of Barrier Function in Cultured Human Corneal Epithelial Cells Induced by Tumor Necrosis Factor-αin a Manner Dependent on NF-κB. (2008)
  • Author(s): Kimura K(その他3名,1番目)
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 565-571
  • Peer Reviewed
• [Journal Article] Role of JNK-Dependent Serine Phosphorylation of Paxillin in Migration of Corneal Epithelial Cells During Wound Closure. (2008)
  • Author(s): Kimura K (その他3名,1番目)
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 125-132
  • Peer Reviewed
• [Journal Article] Protection of human corneal epithelial cells from hypoxia-induced disruption of barrier function by keratinocyte growth factor (2008)
  • Author(s): Teranishi Shinichiro
  • Journal Title: Investigative Ophthalmology & Visual Science 49 Pages: 2432-2437
  • Peer Reviewed
• [Journal Article] Cytokine, Chemokine, and Adhesion Molecule Expression Mediated by Mitogen-Activated Protein Kinases in Human Corneal Fibroblasts Exposed to Poly (I:C) (2008)
  • Author(s): Liu Yang
  • Journal Title: Investigative Ophthalmology & Visual Science 49 Pages: 3336-3344
  • Peer Reviewed
• [Journal Article] Role of JNK-Dependent Serine Phosphorylation of Paxillin in Migration of Corneal Epithelial Cells During Wound Closure. (2008)
  • Author(s): Kazuhiro Kimura
  • Journal Title: Investigative Ophthalmology & Visual Science 49 Pages: 125-132
  • Peer Reviewed
• [Journal Article] Delayed Disruption of Barrier Function in Cultured Human Corneal Epithelial Cells Induced by Tumor Necrosis Factor-a in a Manner Depend ent on NF-kB (2008)
  • Author(s): Kazuhiro Kimura
  • Journal Title: Investigative Ophthalmology & Visual Science 49 Pages: 565-571
  • Peer Reviewed
• [Journal Article] Stimulation of corneal epithelial migration by a synthetic peptide(PHSRN) corresponding to the second cell bindin site of fibronectin. (2007)
  • Author(s): Kimura K (その他8名,1番目)
  • Journal Title: Invest Ophthalmol Vis Sci 48 Pages: 1110-1118
  • Peer Reviewed
• [Presentation] IL-1βによる角膜上皮細胞間接着構造への作用. (2009)
  • Author(s): 木村和博,寺西慎一郎,折田朋子,西田輝夫
  • Organizer: 第33回角膜カンファランス・第25回日本角膜移植学会
  • Place of Presentation: 大阪府
  • Year and Date: 2009-02-19
• [Presentation] IL-1βによる角膜上皮細胞間接着構造への作用 (2009)
  • Author(s): 木村和博
  • Organizer: 第33回角膜カンファランス・第25回日本角膜移植学会
  • Place of Presentation: 大阪府
  • Year and Date: 2009-02-19
• [Presentation] 角膜線維芽細胞でのPoly(I:C)によるサイトカイン産生への作用. (2008)
  • Author(s): 木村和博,劉洋,柳井亮二,近間泰一郎,西田輝夫
  • Organizer: 第45回日本眼感染症学会
  • Place of Presentation: 福岡県
  • Year and Date: 2008-07-05
• [Presentation] 角膜線維芽細胞でのPoly(I:C)によるサイトカイン産生への作用 (2008)
  • Author(s): 木村和博
  • Organizer: 第45回日本眼感染症学会
  • Place of Presentation: 福岡県
  • Year and Date: 2008-07-05
• [Presentation] 炎症性サイトカインTNF-αによるNF-κB依存性角膜上皮バリアー機能の障害 (2008)
  • Author(s): 木村和博
  • Organizer: 第42回日本眼炎症学会
  • Place of Presentation: 福岡県
  • Year and Date: 2008-07-05
• [Presentation] 炎症性サイトカインTNF-αによるNF-κB依存性角膜上皮バリアー機能の障害. (2008)
  • Author(s): 木村和博,寺西慎一郎,福田憲,川本晃司,西田輝夫
  • Organizer: 第42回日本眼炎症学会
  • Place of Presentation: 福岡県
  • Year and Date: 2008-07-04
• [Presentation] 強膜線維芽細胞によるコラーゲンゲル収縮に対するラタノプロストの促進作用. (2008)
  • Author(s): 鈴木克佳,近藤由樹子,木村和博,柳井亮二,石田康仁,能美典正,相良健,西田輝夫
  • Organizer: 第112回日本眼科学会総会
  • Place of Presentation: 神奈川県
  • Year and Date: 2008-04-19
• [Presentation] 強膜線維芽細胞によるコラーゲンゲルの収縮に対するTGF-β1の促進作用. (2008)
  • Author(s): 能美典正,鈴木克佳,近藤由樹子,木村和博,柳井亮二,石田康仁,相良健,西田輝夫
  • Organizer: 第112回日本眼科学会総会
  • Place of Presentation: 神奈川県
  • Year and Date: 2008-04-19
• [Presentation] ギャップ結合構成蛋白質connexin43安定発現角膜上皮細胞の樹立. (2008)
  • Author(s): 木村和博,寺西慎一郎,西田輝夫
  • Organizer: 第32回角膜カンファランス・第24回日本角膜移植学会
  • Place of Presentation: 千葉県
  • Year and Date: 2008-02-29
• [Presentation] 低酸素下におけるKGFの角膜上皮バリアー機能維持作用. (2008)
  • Author(s): 寺西 慎一郎
  • Organizer: 第32回角膜カンファランス・第24回日本角膜移植学会
  • Place of Presentation: 千葉県
  • Year and Date: 2008-02-29
• [Presentation] Cytokine,Chemokine,and adhesion Molecule Ecpression Mediated by Mitogen-Activated Protein Kinases in Human Corneal Fibroblasts Exposed to Poly(I:C). (2007)
  • Author(s): Yang Liu
  • Organizer: 25th Biennial Cornea Research Conference
  • Place of Presentation: USA
  • Year and Date: 2007-10-12
• [Presentation] c-Jun N-terminal kinase Regulates Migration of Corneal Epithelial Cells during Wound closure through Phosphorylation of Paxillin. (2007)
  • Author(s): Kazuhiro Kimura
  • Organizer: ARVO2007 Annual Meeting
  • Place of Presentation: USA
  • Year and Date: 2007-05-08
• [Presentation] JNKによるPaxillinのリン酸化を介した角膜上皮の細胞移動の制御. (2007)
  • Author(s): 木村 和博
  • Organizer: 第111回日本眼科学会総会
  • Place of Presentation: 大阪府
  • Year and Date: 2007-04-20