Regulatory mechanisms for vascular remodeling in hair cycle

• Project/Area Number: 19791316
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: KUBOTA Yoshiaki Keio University, 医学部, 講師 (50348687)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 毛周期 / 血管新生 / 血管リモデリング / LIF / マクロファージ / 毛包幹細胞

Research Abstract

本研究は毛周期を維持する血管リモデリング現象について、この詳細なメカニズムを解明することが目的として遂行された。LIF欠損マウスの血管構築を解析したところ、全身で密な血管網が構築されていた。これは本来血管が出来上がった場所において、VEGF発現がLIF欠損マウスでは著名に亢進していることに起因していた(Kubota et al. J . Clin. Invest. 2008)。また、毛包組織にも多数存在する組織マクロファージが、細胞外マトリックスのリモデリングを介した血管リモデリングに寄与することも解明した(Kubota et al. J. Exp. Med. 2009)。

Research Products

• [Journal Article] Feedback mechanism between blood vessels and astrocytes in retinal vascular development (2009)
  • Author(s): Kubota Y and Suda T
  • Journal Title: Trends Cardiovasc. Med (in press)
• [Journal Article] M-CSF inhibition selectively targets pathological angiogenesis and lymphangiogenesis. (2009)
  • Author(s): Kubota Y, Takubo K, Shimizu T, Ohno H, Kishi K, Shibuya M, Saya H and Suda T*
  • Journal Title: J. Exp. Med. 206 Pages: 1089-1102
• [Journal Article] M-CSF inhibition selectively targets pathological angiogenesis and lymphangiogenesis (2009)
  • Author(s): Kubota Y, et.al.
  • Journal Title: J. Exp. Med. (in press)
  • Peer Reviewed
• [Journal Article] Leukemia inhibitory factor regulates microvessel density by modulating oxygen-dependent VEGF expression in mice. (2008)
  • Author(s): Kubota Y, Hirashima M, Kishi K, Stewart CL and Suda T*.
  • Journal Title: J. Clin. Invest 118(7) Pages: 2393-2403
• [Journal Article] Bone marrow long label-retaining cells reside in the sinusoidal hypoxic niche (2008)
  • Author(s): Kubota Y, Takubo K and Suda T*
  • Journal Title: Biochem. Biophys. Res. Commun. 366 Pages: 335-339
• [Journal Article] Ontogeny and Multipotency of Neural Crest-Derived Stem Cells in Bone Marrow, Dorsal Root Ganglia and Whisker Pad of Adult Rodents. (2008)
  • Author(s): Nagoshi N, Shibata S, Kubota Y, Nakamura M, Nagai Y, Satoh E, Morikawa S, Okada Y, Mabuchi Y, Katoh H, Okada S, Fukuda K, Suda T, Matsuzaki Y, Toyama Y, Okano H
  • Journal Title: Cell Stem Cell 2 Pages: 392-403
• [Journal Article] Leukemia inhibitory factor regulates microvessel density by modulating oxygen-dependent VEGF expression in mice (2008)
  • Author(s): Kubota Y, et.al.
  • Journal Title: J. Clin. Invest 118(7) Pages: 2393-2403
  • Peer Reviewed
• [Journal Article] Leukemia inhibitory factor regulates microvessel density by modulatingoxygen-dependent VEGF expression. (2008)
  • Author(s): Kubota Y, et. al.
  • Journal Title: J. Chin. Invest. (in press)
  • Peer Reviewed
• [Journal Article] Bone marrow long label-retaining cells reside in the sinusoidal hypoxicniche. (2008)
  • Author(s): Kubota Y, et. al.
  • Journal Title: Biochem. Biopbys. Res. Commun. 366 Pages: 335-339
  • Peer Reviewed
• [Journal Article] Ontogeny and Multipotency of Neural Crest-Derived Stem Cells in Bone Marrow, Dorsal Root Ganglia and Whisker Pad of Adult Rodents. (2008)
  • Author(s): Nagoshi N, et. al.
  • Journal Title: Cell Stem Cell. 2 Pages: 392-403
  • Peer Reviewed
• [Journal Article] Mutations in CD96, a member of the immunoglobulin superfamily, cause a form of the C(Opitz Trigonocephaly)syndrome (2007)
  • Author(s): Kaname T, Yanagi K, Chinen Y, Makita Y, Okamoto N, Maehara H, Owan I, Kanaya F, Kubota Y, Oike Y, Yamamoto T, Kurosawa K, Fukushima Y, Bohring A, Opitz JM, Yoshiura K, Niikawa N, Naritomi K.
  • Journal Title: Am. J. Hum. Genet 81 Pages: 835-841
• [Journal Article] Reactive oxygen species induce chondrocyte hypertrophy in endochondral ossification. (2007)
  • Author(s): Morita K, Miyamoto T, Fujita N, Kubota Y, Ito K, Takubo K, Miyamoto K, Ninomiya K, Suzuki T, Iwasaki R, YagiM, Takaishi H, Toyama Y, Suda T
  • Journal Title: J. Exp. Med. 204 Pages: 1613-1623
• [Journal Article] Mutations in CD96, a member of the immunoglobulin superfamily, cause a form of the C (Opitz Trigonocephaly) syndrome. (2007)
  • Author(s): Kaname T, et. al.
  • Journal Title: Am J. Hum. Genet. 81 Pages: 835-841
  • Peer Reviewed
• [Journal Article] Reactive oxygen species induce chondrocyte hypertrophy in endochondralossification. (2007)
  • Author(s): Morita K, et. al.
  • Journal Title: J. Exp. Med. 204 Pages: 1613-1623
  • Peer Reviewed
• [Presentation] M-CSF Inhibition Selectively Targets Tumor Angiogenesis and Lymphan giogenesis (2008)
  • Author(s): Kubota Y
  • Organizer: 50th American Society of Hematology (ASH) Annual Meeting
  • Place of Presentation: San Francisco
  • Year and Date: 2008-12-07
• [Presentation] M-CSF阻害は腫瘍選択的に血管・リンパ管新生を阻害し遠隔転移を抑制する (2008)
  • Author(s): 久保田義顕、須田年生
  • Organizer: 第6回血液血管オルビス
  • Place of Presentation: 東京
  • Year and Date: 2008-08-09
• [Presentation] 血管新生部位に動員される骨髄由来細胞の役割 (2008)
  • Author(s): 久保田義顕
  • Organizer: 慶應臨床血管フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2008-05-31
• [Presentation] M-CSF Inhibition Selectively Targets Tumor Angiogenesis and Lymphangiogenesis (2008)
  • Author(s): Kubota Y and Suda T.
  • Organizer: 50th American Society of Hematology(ASH)Annual Meeting.
  • Place of Presentation: San Francisco
• [Presentation] Po marks angioblasts in Notch-dependent retinal vascular development. (2008)
  • Author(s): Kubota Y and Suda T
  • Organizer: 8th ESH Euroconference on Angiogenesis.
  • Place of Presentation: Paris, France
• [Presentation] Leukemia inhibitory factor regulates microvessel density by modulating oxygen-dependent VEGF expression (2008)
  • Author(s): Kubota Y and Suda T
  • Organizer: The U.S.-Japan Cooperative Cancer Research Program Workshop.
  • Place of Presentation: Kyoto Heian Kaikan, Kyoto
• [Presentation] 血管新生部位に動員される骨髄由来細胞の役割 (2007)
  • Author(s): 久保田義顕、須田年生
  • Organizer: BMB2007BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会合同大会)ワークショップ「血管・リンパ管発生の新展開」
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 血管新生部位に動員される骨髄由来細胞の役割 (2007)
  • Author(s): 久保田 義顕、須田 年生
  • Organizer: BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会)ワークショップ「血管・リンパ管発生の新展開」
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2007-12-14
• [Presentation] LIF regulates endothelial tip cell activity in an oxygen-independent manner (2007)
  • Author(s): Kubota Y and Suda T
  • Organizer: 21st Century Center of Excellence (COE) Program
  • Year and Date: 2007-11-29
• [Presentation] 血管新生部位に動員される骨髄由来細胞の役割 (2007)
  • Author(s): 久保田義顕、須田年生
  • Organizer: 第3回SummerVascular Conference(SVC)
  • Place of Presentation: 東京
  • Year and Date: 2007-09-08
• [Presentation] 骨髄由来細胞の血管新生における生理的役割 (2007)
  • Author(s): 久保田義顕、須田年生
  • Organizer: 第28回炎症再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2007-08-02
• [Presentation] Leukemia inhibitory factor negatively regulates VEGF expression in an oxygen-independent manner. (2007)
  • Author(s): Kubota Y and Suda T
  • Organizer: The First Pacific Symposium on Angiogenesis and Lymphangiogenesis
  • Place of Presentation: Jeju Republic of Korea.
• [Presentation] Critical role of bone marrow-derived perivascular cell in vascularstability. (2007)
  • Author(s): 久保田義顕、須田年生
  • Organizer: 5th Aso International Meeting (AIM)
  • Place of Presentation: 熊本
• [Presentation] Leukemia inhibitory factor plays a key role in endothelial feedback mechanism against hypoxia induced pro-angiogenic pathway (2007)
  • Author(s): 久保田義顕、須田年生
  • Organizer: 5th Aso InternationalMeeting(AIM)
  • Place of Presentation: 熊本
• [Presentation] Endothelial Tip Cell Activity Is Negatively Regulated by Leukemia Inhibitory Factor in an Oxygen-Independent Manner. (2007)
  • Author(s): Kubota Y and Suda T
  • Organizer: 7th ESH Euroconference on Angiogenesis
  • Place of Presentation: Albufeira, Portugal
• [Presentation] Endothelial Tip CeU Activity Is Negatively Regulated by Leukemia Inhibitory Factor in an Oxygen-Independent Manner. (2007)
  • Author(s): Kubota Y and Suda T
  • Organizer: 7th ESH Euroconference on Angiogenesis.
  • Place of Presentation: Albufeira, Portugal
• [Presentation] Leukemia inhibitory factor negatively regulates VEGF expression in anoxygen-independent manner. (2007)
  • Author(s): Kubota Y and Suda T
  • Organizer: The First Pacific Symposium on Angiogenesis and Lymphangiogenesis
  • Place of Presentation: Jeju, Republic of Korea.
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/celldiff/works.html
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/celldiff/works.html