Elucidation of the mechanisms of ischemic reperfusion cardiac injury by RNA interference, and clinical application for gene thrapy

• Project/Area Number: 19791332
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Emergency medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KATOH Yuko Kyoto Prefectural University of Medicine, 附属病院, 専攻医 (50398400)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,600,000)
• Keywords: 心筋虚血再潅流 / RNA干渉法 / 遺伝子治療

Research Abstract

ラット心筋細胞にsiRNAを導入する実験は、In Vitro系はNucleofection法にて、 In Vivo系はHVJ Envelope Vectorを用いて行い、Real Time PCR及びWestern Blotで成功したことを確認した。p38MAP kinaseをノックダウンすることにより、サイトカイン放出及び心筋収縮力が低下することは、In Vitroの実験では確認した。In Vivo系において、心筋梗塞の減少効果については、今後実験結果を確認する予定である。

Research Products

• [Journal Article] 食道閉鎖症、大動脈縮窄症に高度の先天性気管狭窄症を合併し、呼吸管理に難渋した1症例 (2008)
  • Author(s): 谷口彩乃, 梅内貴子, 志馬伸朗, 加藤祐子, 橋本悟
  • Journal Title: 日本集中治療医学会雑誌 15(4) Pages: 539-542
  • NAID: 10024841272
• [Journal Article] A randomized trial of ultrasound image-based skin surface marking versus real-time ultrasound-guided internal jugular vein catheterization in infants (2007)
  • Author(s): Hosokawa K, Shime N, Kato Y, Hashimoto S.
  • Journal Title: Anesthesiology 107(5) Pages: 720-724
• [Journal Article] Glycopeptide pharmacokinetics in current paediatric cardiac surgery practice. (2007)
  • Author(s): Shime N, Kato Y, Kosaka T, Kokufu T, Yamagishi M, Fujita N.
  • Journal Title: Eur J Cardiothorac Surg 32(4) Pages: 577-578
• [Journal Article] Effects of controlled perioperative antimicrobial prophylaxis on infectious outcomes in pediatric cardiac surgery. (2007)
  • Author(s): Kato Y, Shime N, Hashimoto S, Nomura M, Okayama Y, Yamagishi M, Fujita N.
  • Journal Title: Crit Care Med 35(7) Pages: 1763-1768
• [Journal Article] A randomized trial of ultrasound image-hased skin surface marking versus real-time ultrasound-guided internal jugular vein catheterization in infants. (2007)
  • Author(s): Hosokawa K, et. al.
  • Journal Title: Anesthesiology. 107 Pages: 720-4
  • Peer Reviewed
• [Journal Article] Glycopeptide pharmacokinetics in current paediatric cardiac surgery practice. (2007)
  • Author(s): Shime N, et. al.
  • Journal Title: Eur J Cardiothorac Surg. 32 Pages: 577-81
  • Peer Reviewed
• [Journal Article] Effects of controlled perioperative antimicrobial prophylaxis on infectious outcomes in pediatric cardiac surgery. (2007)
  • Author(s): Kato Y, et. al.
  • Journal Title: Crit Care Med. 35 Pages: 1763-8
  • Peer Reviewed
• [Presentation] 肺高血圧を有する乳児先天性心疾患根治術後での早期抜管は術後炎症反応を軽減する (2008)
  • Author(s): 梅内貴子, 志馬伸朗, 加藤祐子, 橋本悟
  • Organizer: 第30回日本呼吸療法医学会総会
  • Place of Presentation: 松本
  • Year and Date: 2008-07-04
• [Presentation] 小児静脈穿刺におけるトランスイルミネーション法の有用性を評価するパイロットスタディ (2008)
  • Author(s): 貴志千春, 加藤祐子, 志馬伸朗
  • Organizer: 第55回日本麻酔科学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-12
• [Presentation] 診断治療に難渋した小児膿胸の一例 (2008)
  • Author(s): 梅内貴子, 志馬伸朗, 青木智史, 小坂喜太郎, 橋本壮志, 加藤祐子, 黄瀬ひろみ, 木村彰夫, 天谷文昌, 橋本悟
  • Organizer: 第12回京滋救命救急セミナー
  • Place of Presentation: 京都
  • Year and Date: 2008-03-21