The elucidation of the mechanisms of dexamethasone-regulated osteoblast differentiation

• Project/Area Number: 19791351
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Nihon University
• Principal Investigator: MIKAMI Yoshikazu Nihon University, 歯学部, 助教 (80434075)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,860,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥660,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: デキサメサゾン / 骨芽細胞 / BMP-2 / STAT3

Research Abstract

In this study, we investigated the mechanisms by which dexamethasone (Dex) affects osteoblast differentiation. This study demonstrated that Dex regulated osteoblast differentiation on the cell-differentiation stage dependent manner, and that MAP kinase and Jak-STAT signaling pathway were involved in the mechanisms for the Dex-regulated osteoblast differentiation.

Research Products

• [Journal Article] Bone morphogenetic protein 2 and Dexamethasone synergistically increase Alkaline Phosphatase levels through JAK/STAT signaling in C3H10T1/2 cells. (2010)
  • Author(s): Mikami Y, Asano M, Honda J M, Takagi M.
  • Journal Title: Journal of Cellular Physiology. 223 Pages: 123-133
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein 2 and dexamethasone synergistically increase alkaline phosphatase levels through JAK/STAT signaling in C3H10T1/2 cells (2010)
  • Author(s): Mikami Y, Asano M, Honda MJ, Takagi M.
  • Journal Title: Journal of Cellular Physiology 223(1) Pages: 123-133
  • Peer Reviewed
• [Journal Article] A new synthetic compound, SST-EDI-1 inhibits osteoblast differentiation by down-regulating Osterix expression. (2009)
  • Author(s): Mikami Y, Somei M, Takagi M.
  • Journal Title: The Journal of Biochemistry. 145 Pages: 239-247
  • Peer Reviewed
• [Journal Article] A tryptamine derivative, SST-EDI-1, inhibits apoptosis and stimulates mineralization in osteoblasts. (2009)
  • Author(s): Mikami Y, Somei M, Takagi M.
  • Journal Title: Endocrine Journal. 56 Pages: 665-678
  • Peer Reviewed
• [Journal Article] Dexamethasone promotes DMP-1 mRNA expression by inhibiting negative regulation of Runx2 in multipotential mesenchymal progenitor, ROB-C26. (2008)
  • Author(s): Mikami Y, Takahashi T, Takagi M.
  • Journal Title: Cell Biology International. 32 Pages: 239-246
  • Peer Reviewed
• [Journal Article] Inductive effects of dexamethasone on the mineralization and the osteoblastic gene expressions in mature osteoblast-like ROS17/2. 8 cells. (2007)
  • Author(s): Mikami Y, Omoteyama Y, Takagi M.
  • Journal Title: Biochemical and biophysical research communications. 362 Pages: 368-373
  • Peer Reviewed
• [Journal Article] Dexamethasone promotes DMP-1 mRNA expression by inhibiting negative regulation of Runx2 in multipotential mesenchymal progenitor, ROB-C26. (2007)
  • Author(s): Yoshikazu Mikami
  • Journal Title: Cell Biology International 32・2 Pages: 239-246
  • Peer Reviewed
• [Journal Article] Inductive effects of dexamethasone on the mineralization and the osteoblastic gene expressions in mature osteoblast-like ROS17/2.8 cells. (2007)
  • Author(s): Yoshikazu Mikami
  • Journal Title: Biochemical and biophysical research unications 362・2 Pages: 368-373
  • Peer Reviewed
• [Presentation] ROS17/2.8細胞における石灰化と骨芽細胞分化関連因子の発現に対するデキサメサゾンの影響 (2008)
  • Author(s): 三上 剛和
  • Organizer: 第113回日本解剖学会全国学術集会
  • Place of Presentation: 大分大学医学部
  • Year and Date: 2008-03-29