Association of oral streptococci with the host cells in the pathogenesis of infective endocariditis

• Project/Area Number: 19791352
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: YAJIMA Ayako The Nippon Dental University, 生命歯学部, 助教 (00287773)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,710,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: Streptococcus gordonii / 感染性心内膜炎 / アドへジン / レセプター / S. gordonii / アドヘジン / シアル酸 / HL-60 / 赤血球

Research Abstract

Streptococcus gordoniiは、歯垢を形成する細菌の一つで、感染性心内膜炎の原因菌としても注目されている。感染性心内膜炎の発症には、局所で菌体と血球系の細胞が結合し、疣贅といわれる大きな固まりを作ることが重要だと考えられている。そこで、これらの宿主細胞とS. gordoniiの付着様式や各宿主細胞のレセプターの同定を試みた。その結果、赤血球では主にGlycophorin Aとband3、単球とマクロファージではCD11bとCD43、好中球ではCD11b、CD43とCD50がレセプターであることが明らかとなった。

Research Products

• [Journal Article] Binding of the Streptococcus gordonii DL1 surface protein Has to the host cell membrane glycoproteins CD11b, CD43, and CD50 (2008)
  • Author(s): Yumiko Urano-Tashiro, Ayako Yajima, Eizo Takashima, Yukihiro Takahashi, and Kiyoshi Konishi
  • Journal Title: Infection and Immunity 76 Pages: 4986-4991
  • Peer Reviewed
• [Journal Article] Has, an adhesin of Streptococcus gordonii DL1, binds to a2-3 Linked sialic acid on Glycophorin A of the erythrocyte membrane. (2008)
  • Author(s): Ayako Yajima, Yumiko Urano, Kisaki Shimazu, Eizo Takashima, Yukihiro Takahashi, and Kiyoshi Konishi.
  • Journal Title: Microbiology and Immunology 52 Pages: 69-77
  • Peer Reviewed
• [Journal Article] Binding of the Streptococcus gordonii DL1 surface protein Hsa to the host cell membrane glycoproteins CD11b, CD43, and CD50 (2008)
  • Author(s): Yumiko Urano-Tahiro, Ayako Yajima, Eizo Takashima, Yukihiro Takahashi, and Kiyoshi Konishi
  • Journal Title: Infection and Immunity 76 Pages: 4986-91
  • Peer Reviewed
• [Journal Article] Hsa, an Adhesin of Streptococcus gordonii DL1, binds to α2-3-Linked Sialic Acid on Glycophorin A of the Erythrocyte Membrane (2008)
  • Author(s): Ayako Yajima, Yumiko Urano, Kisaki Shimazu, Eizo Takashima, Yukihiro Takahashi and Kiyoshi Konishi
  • Journal Title: Microbiology and Immunology 52 Pages: 69-77
  • NAID: 10025691165
  • Peer Reviewed
• [Presentation] Erythrocyte membrane receptors for Streptococcus gordonii sialic acid-binding adhesin (2008)
  • Author(s): Ayako Yajima, Yukihiro Takahashi, Yumiko Urano-Tashiro, Kisaki Shimazu, Eizo Takashima, and Kiyoshi Konishi
  • Organizer: International Association for Dental Research 86th General Session & Exhibition
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] Erythrocyte Membrane Receptors for Streptococcus gordonii Sialic AcidBinding Adhesin (2008)
  • Author(s): Ayako Yajima, Yukihiro Takahashi, Yumiko Urano-Tashiro, Kisaki Shimazu, Eizo Takashima, and Kiyoshi Konishi
  • Organizer: International Association for Dental Research 86th General Session & Exhibition
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] Streptococcus gordonii DL1株シアル酸結合性アドへジンの赤血球レセプターの同定 (2008)
  • Author(s): 矢島彩子、高橋幸裕、浦野有美子、島津貴咲、高島英造、古西清司
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-03-24
• [Presentation] Streptococcus gordonii DL1株シアル酸結合性アドヘジンの赤血球レセプターの同定 (2008)
  • Author(s): 矢島彩子、高橋幸裕、浦野有美子、島津貴咲、高島英造、古西清司
  • Organizer: 第81回 日本細菌学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-03-24
• [Presentation] Streptococcus gordonii DL1株シアル酸結合性アドへジンの赤血球レセプターの同定. (2007)
  • Author(s): 矢島彩子、浦野有美子、島津貴咲、高島英造、高橋幸裕、古西清司
  • Organizer: 第49回歯科基礎医学会総会.
  • Place of Presentation: 北海道大学
  • Year and Date: 2007-08-31
• [Presentation] Streptococcus gordonii DL1株シアル酸結合性アドヘジンの赤血球レセプターの同定 (2007)
  • Author(s): 矢島彩子、浦野有美子、島津貴咲、高島英造、高橋幸裕、古西清司
  • Organizer: 第49回 歯科基礎医学会総会
  • Place of Presentation: 北海道大学
  • Year and Date: 2007-08-31