Identification of expression and localization of osteoclast-specific ABC transporters

• Project/Area Number: 19791361
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: NAKAGAWA Hiroshi Tokyo Institute of Technology, 大学院・生命理工学研究科, 助教 (40397039)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,610,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 口腔生化学 / 破骨細胞 / ABCトランスポーター / ABCG2 / 一塩基多型 / N結合型糖鎖 / ユビキチン / プロテアソーム / ABCトランスホーター / 破皮骨細胞

Research Abstract

The expression of nine and four of 49 ABC transporters were found to be up- and down-regulated during osteoclastgenesis, respectively. It was also found that the expression levels and distribution of ABCG2 were regulated by single nucleotide polymorphisms and posttranslational modifications, which was awarded the Special ABC2010 Poster Recognition Award.

Research Products

• [Journal Article] Disruption of N-linked glycosylation enhances ubiquitin-mediated proteasomal degradation of human ATP-binding cassette transporter ABCG2. (2009)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Tamura, A., Koshiba, S., Toyoda, Y., Ishikawa, T.
  • Journal Title: FEBS J. 276 Pages: 7237-7252
  • Peer Reviewed
• [Journal Article] Quality control of human ABCG2 protein in the endoplasmic reticulum: Ubiquitination and proteasomal degradation. (2009)
  • Author(s): Wakabayashi-Nakao, K., Tamura, A., Furukawa, T, Nakagawa, H., Ishikawa, T.
  • Journal Title: Advanced Drug Delivery Reviews 61 Pages: 66-72
  • Peer Reviewed
• [Journal Article] Major SNP (Q141K) Variant of Human ABC Transporter ABCG2 Undergoes Lysosomal and Proteasomal Degradations. (2009)
  • Author(s): Furukawa T, Wakabayashi K, Tamura A, Nakagawa, H., Morishima Y, Osawa Y, Ishikawa T
  • Journal Title: Pharm. Res. 26 Pages: 469-479
  • Peer Reviewed
• [Journal Article] Disruption of N-linked glycosylation enhances ubiquitin-mediated proteasomal degradation of human ATP-binding cassette transporter ABCG2 (2009)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Tamura, A., Koshiba, S., Toyoda, Y., Ishikawa, T.
  • Journal Title: FEBS J. 276 Pages: 7237-7252
  • Peer Reviewed
• [Journal Article] Major SNP (Q141K) Variant of Human ABC Transporter ABCG2 Undergoes Lysosomal and Proteasomal Degradations. (2009)
  • Author(s): Furukawa T, Wakabayashi K, Tamura A, Nakagawa, H., Morishima Y, Osawa Y, and Ishikawa T.
  • Journal Title: Pharmaceutical Research 26 Pages: 469-479
  • Peer Reviewed
• [Journal Article] Quality control of human ABCG2 protein in the endoplasmic reticulum : Ubiquitination and proteasomal degradation (2009)
  • Author(s): Wakabayashi-Nakao, K., Tamura, A., Toyoda, Y, Koshiba, S., Nakagawa, H., and Ishikawa, T.
  • Journal Title: Advanced Drug Delivery Reviews 61 Pages: 66-72
  • Peer Reviewed
• [Journal Article] Evidence for ubiquitin-mediated proteasomal degradation of non-synonymous SNP Variants. (2008)
  • Author(s): Nakagawa, H., Tamura, A., Wakabayashi, K., Hoshijima, K., Komada, M., Yoshida, T., Kometani, S., Matsubara, T., Mikuriya, K., Ishikawa, T.
  • Journal Title: Biochemical J. 411 Pages: 623-631
  • Peer Reviewed
• [Journal Article] Evidence for ubiquitin-mediated proteasomal degradation of non-synonymous SNP Variants. (2008)
  • Author(s): Hiroshi Nakagawa
  • Journal Title: Biochemical Journal 411 Pages: 623-631
  • Peer Reviewed
• [Presentation] The impacts of posttranslational modifications and non-synonymous SNPs on the protein stability of human ABC transporter ABCG2 protein in culture cells (2010)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Tamura, A., Koshiba, S., Toyoda, Y., Furukawa, T., Ishikawa, T
  • Organizer: The FEBS-ABC2010 - 3rd FEBS Special Meeting on ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases
  • Place of Presentation: インスブルック(オーストリア)
• [Presentation] The impacts of posttranslational modifications and non-synonymous SNPs on the protein stability of human ABC transporter ABCG2 protein in culture cells (2010)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Tamura, A., Koshiba, S., Toyoda, Y., Furukawa, T., Ishikawa, T.
  • Organizer: The FEBS-ABC2010-3rd FEBS Special Meeting on ATP-Binding Cassette(ABC)Proteins : From Multidrug Resistance to Genetic Diseases
  • Place of Presentation: インスブルック(オーストリア)
• [Presentation] Monomeric non-glycosylated form of human ATP-binding cassette transporter ABCG2 is the most fragile in culture cells (2009)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Tamura, A., Koshiba, S., Toyoda, Y., Ishikawa, T
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] N-linked glycosylation stabilizes the de novo synthesized human ABC transporter ABCG2 protein (2009)
  • Author(s): Nakagawa, H., Wakabayashi, K., Tamura, A., Koshiba, S., Toyoda, Y., Ishikawa
  • Organizer: 日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03
• [Presentation] The impact of N-linked glycosylation on the protein stability of human ABC transporter ABCG2 protein (2009)
  • Author(s): Nakagawa, H., Wakabayashi-Nakao, K., Tamura, A., Koshiba, S., Toyoda, Y., Ishikawa, T
  • Organizer: 2nd DKFZ-NCC Workshop on Cancer Research Tokyo
  • Place of Presentation: 東京
  • Year and Date: 2009-07-08
• [Presentation] Impact of Major Non-synonymous SNP (Q141K) on Proteolysis of Human ABC Transporter ABCG2 via Lysosomal and Ubiquitin-mediated Proteasomal Degradation Pathways (2008)
  • Author(s): 古川朋佳, 若林香菜子, 田村藍, 中川大, 石川智久
  • Organizer: 日本分子生物学会・日本生化学会合同大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Disruption of N-linked glycosylation enhances ubiquitin-mediated proteasomal degradation of human ATP-binding cassette transporter ABCG2 (2008)
  • Author(s): 中川大, 若林香菜子, 田村藍, 小柴梢子, 豊田優, 石川智久
  • Organizer: 日本分子生物学会・日本生化学会合同大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Impact of Major Non-synonymous SNP (Q141K) on Proteolysis of Human ABC Transporter ABCG2 via Lysosomal and Ubiquitin-mediated Proteasomal Degradati on Pathways (2008)
  • Author(s): 古川朋佳, 若林香菜子, 田村藍, 中川大, 石川智久
  • Organizer: 日本分子生物学会・日本生化学会合同大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Genetic polymorphisms of human ABCG2 and ER-associated degradation (ERAD) pathway (2008)
  • Author(s): Wakabayashi, K., Tamura, A., An, R., Hoshijima, K., Nakagawa, H., Ishikawa, T
  • Organizer: 2nd FEBS Special Meeting Co-sponsored by FEMS ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases
  • Place of Presentation: インスブルック(オーストリア)
• [Presentation] Genetic polymorphisms of human ABCG2 and ER-associated degradation(ERAD)pathway. (2008)
  • Author(s): Kanako Wakabayashi
  • Organizer: 2nd FEBS Special Meeting Co-sponsored by FEMS ATP-Binding Cassette (ABC) Proteins
  • Place of Presentation: Innsbruck
• [Presentation] Evidence for ubiquitination and degradation of non-synonymous SNP proteins of human ATP-binding cassette transporter ABCG2 (2007)
  • Author(s): Nakagawa, H., Tamura, A., Wakabayashi, K., Ishikawa, T
  • Organizer: 日本分子生物学会・日本生化学会合同大会(BMB2007)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] Evidence for ubiquitination and degradation of non-synonymous SNPproteins ofhuman ATP-binding cassette transporter ABCG2. (2007)
  • Author(s): Hiroshi Nakagawa
  • Organizer: 日本分子生物学会・日本生化学会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] ヒトABCトランスポーターABCG2のタンパク質品質管理と細胞内分解機構:一塩基多型と翻訳後修飾の影響 (2007)
  • Author(s): 中川大, 若林香菜子, 田村藍, 星島一幸, 石川智久
  • Organizer: トランスポーター研究会関東支部会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-10
• [Presentation] ヒトABCトランスポーターABCG2のタンパク質品質管理と細胞内分解機構:一塩基多型と翻訳後修飾の影響. (2007)
  • Author(s): 中川 大
  • Organizer: トランスポーター研究会関東支部会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-10
• [Presentation] ヒトABCトランスポーターの遺伝子多型研究の最前線ABCBl (P-gp/MDRl), ABCG2 (BCRP/MXR), ABCBll (BSEP). (2007)
  • Author(s): 石川 智久
  • Organizer: トランスポーター研究会関東支部会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-10
• [Remarks]
  • URL: http://www.ishikawa-lab.bio.titech.ac.jp/index.html
• [Remarks]
  • URL: http://www.ishikawa-lab.bio.titech.ac.jp/index.html