Identification of osteoclast niche using RANKL-deficient and M-CSF-deficient mice

• Project/Area Number: 19791376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: NAKAMICHI Yuko Matsumoto Dental University, 総合歯科・医学研究所, 助教 (20350829)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,590,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: M-CSF / RANKL / 破骨細胞ニッチ / IL-34 / 活性型ビタミンD3 / 脾臓

Research Abstract

細胞周期が停止した破骨細胞前駆細胞(pOCP ; post-mitotic osteoclast precursors)は、破骨細胞ニッチを形成する。pOCPの存在部位(すなわち破骨細胞ニッチ)を同定するため、pOCPをGFP標識できるトランスジェニック(Tg)マウスを開発するため、DNAコンストラクトを作製した。また本研究によりM-CSF受容体の新しいリガンドIL-34が、破骨細胞ニッチからの破骨細胞供給に関与することを示唆するデータを得た。

Research Products

• [Journal Article] Dental pulp and periodontal ligament cells support osteoclast differentiation (2009)
  • Author(s): Uchiyama M, Nakamichi Y, Nakamura M, Kinugawa S, Yamada H, Udagawa N, Miyazawa H.
  • Journal Title: J Dent Res (in press)
  • Peer Reviewed
• [Journal Article] Evaluation of Pharmaceuticals With a Novel Fifty-Hour Animal Model of Bone Loss (2009)
  • Author(s): Tomimori Y, Mori K, Koide M, Nakamichi Y, Ninomiya T, Udagawa N, Yasuda H.
  • Journal Title: J Bone Miner Res (in press)
  • Peer Reviewed
• [Journal Article] Identification of cell cycle-arrested quiescent osteoclast precursors in vivo (2009)
  • Author(s): Mizoguchi T, Muto A, Udagawa N, Arai A, Yamashita T, Hosoya A, Ninomiya T, Nakamura H, Yamamoto Y, Kinugawa S, Nakamura M, Nakamichi Y, Kobayashi Y, Nagasawa S, Oda K, Tanaka H, Tagaya M, Penninger JM, Ito M, Takahashi N.
  • Journal Title: J Cell Biol 184 Pages: 541-54
  • Peer Reviewed
• [Journal Article] Docetaxel inhibits bone resorption through suppression of osteoclast formation and function in different manners (2009)
  • Author(s): Takahashi M, Mizoguchi T, Uehara S, Nakamichi Y, Yang S, Naramoto H, Yamashita T, Kobayashi Y, Furusawa K, Udagawa N, Uematsu T, Takahashi N.
  • Journal Title: Bone Miner Metab 27 Pages: 24-35
  • NAID: 10025336084
  • Peer Reviewed
• [Journal Article] Identification of cell cycle-arrested quiescent osteoclast precursors in vivo (2009)
  • Author(s): Toshihide Mizoguchi
  • Journal Title: J Cell Biol 23 Pages: 541-54
  • Peer Reviewed
• [Journal Article] Docetaxel inhibits bone resorption through suppression of osteoclast formation and function in different manners (2009)
  • Author(s): Masahiro Takahashi
  • Journal Title: J Bone Miner Metab 27 Pages: 24-35
  • Peer Reviewed
• [Journal Article] Evaluation of Pharmaceuticals With a Novel Fifty-Hour Animal Model of Bone Loss (2009)
  • Author(s): Yoshiya Tomimori
  • Journal Title: J Bone Miner Res (印刷中)
  • Peer Reviewed
• [Journal Article] Altered fracture callus formation in chondromodulin-I deficient mice (2008)
  • Author(s): Yukata K, Matsui Y, Shukunami C, Takimoto A, Goto T, Nishizaki Y, Nakamichi Y, Kubo T, Sano T, Kato S, Hiraki Y, Yasui N.
  • Journal Title: Bone 43 Pages: 1047-56
  • Peer Reviewed
• [Journal Article] Altered fracture callus formation in chondromodulin-I deficient mice (2008)
  • Author(s): Kiminori Yutaka
  • Journal Title: Bone 43 Pages: 1047-56
  • Peer Reviewed
• [Journal Article] Osteoprotegerin reduces the serum level of receptor activator of NF-κB ligand derived from osteoblasts. (2007)
  • Author(s): Yuko Nakamichi
  • Journal Title: Journal of Immunology 178 Pages: 192-200
  • Peer Reviewed
• [Journal Article] Strain-dependent embryonic lethality and exaggerated vascular remodeling in heparin cofactor II-deficient mice. (2007)
  • Author(s): Ken-ichi Aihara
  • Journal Title: The Journal of Clinical Investigation 117 Pages: 1514-1526
  • Peer Reviewed
• [Journal Article] New 19-nor-(20S)-1α,25-dihydroxyvitamin D_3 analogs strongly stimulate osteoclast formation both in vivo and in vitro. (2007)
  • Author(s): Masahiro Sato
  • Journal Title: Bone 40 Pages: 293-304
  • Peer Reviewed
• [Journal Article] Interleukin-4 inhibition of osteoclast differentiation is stronger than that of interleukin-13 and they are equivalent for induction of osteoprotegerin production from osteoblasts. (2007)
  • Author(s): Atsushi Yamada
  • Journal Title: Immunology 140 Pages: 573-579
  • Peer Reviewed
• [Journal Article] Osteoblasts play important roles in osteoclastogenesis through offering the critical microenvironment for the action of RANKL. (2007)
  • Author(s): Nobuyuki Udagawa
  • Journal Title: Dentistry in Japan 43 Pages: 195-207
  • Peer Reviewed
• [Journal Article] OSTM1とCLC-7. (2007)
  • Author(s): 中道 裕子
  • Journal Title: 骨粗鬆症治療 6 Pages: 262-265
• [Presentation] An M-CSF-independent mechanism for osteoclastogenesis (2008)
  • Author(s): 中道裕子
  • Organizer: 第5回Bone Biology Forum
  • Place of Presentation: 静岡(富士教育研修所)
  • Year and Date: 2008-08-26
• [Presentation] An M-CSF-independent mechanism for osteoclastogenesis (2008)
  • Author(s): 中道裕子
  • Organizer: 第5回Bone Biology Forum
  • Place of Presentation: 静岡
• [Presentation] M-CSF independent mechanisms for osteoclastogenesis. (2007)
  • Author(s): Yuko Nakamichi
  • Organizer: 29th American Society for Bone and Mineral Research (ASBMR) Annual Meeting
  • Place of Presentation: ハワイ, USA
  • Year and Date: 2007-09-19
• [Presentation] M-CSF非存在下における破骨細胞形成メカニズムの解析 (2007)
  • Author(s): 中道 裕子
  • Organizer: 日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-07-19
• [Presentation] M-CSF independent mechanisms for osteoclastogenesis (2007)
  • Author(s): Nakamichi Y, Udagawa N, Yasuda H, Nakamura M, Kobayashi Y, Takahashi N Nakamichi Y, Udagawa N, Yasuda H, Nakamura M, Kobayashi Y, Takahashi N
  • Organizer: (J Bone Miner Res 22, Suppl 1; S380, 2007) 29th American Society fo Bone and Mineral Research (ASBMR) Annual Meeting
  • Place of Presentation: モントリオール
• [Presentation] M-CSF非存在下における破骨細胞形成メカニズムの解析 (2007)
  • Author(s): 中道裕子,宇田川信之,林眞一,保田尚孝,中村美どり,小林泰浩,高橋直之
  • Organizer: (第25回日本骨代謝学会プログラム抄録集: p.228)日本骨代謝学会(第25回)
  • Place of Presentation: 大阪
• [Presentation] In vivoにおいて細胞周期の停止した破骨細胞前駆細胞(pOCP)は5-FU悲感受性である (2007)
  • Author(s): 溝口利英,武藤昭紀,細矢明宏,中道裕子,山下照仁,小林泰浩,宇田川信之,伊藤充雄,高橋直之
  • Organizer: (第25回日本骨代謝学会プログラム抄録集: p.275)日本骨代謝学会(第25回)
  • Place of Presentation: 大阪
• [Remarks]
  • URL: http://www.mdu.ac.jp/laboratory/research_contents/index.html
• [Remarks]
  • URL: http://www.mdu.ac.jp/laboratory/research_contents/649/000647.html
• [Remarks]
  • URL: http://www.mdu.ac.jp/laboratory/research_contents/649/000647.html