nvestigation of the behavior of nuclear proteins in human cancer cells

• Project/Area Number: 19791383
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Kyushu Dental College
• Principal Investigator: KITO Shinji Kyushu Dental College, 歯学部, 助教 (80347682)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥2,780,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥700,000 (Direct Cost: ¥700,000)
• Keywords: 悪性腫瘍細胞 / アポトーシス / AgNORs / ニュークレオリン / ニュークレオフォスミン / UV照射 / nucleolin / nucleophosmin / RNAi / FDG-PET / nucleophosmn / 癌幹細胞 / iPS細胞 / 抗癌剤 / クロマチン

Research Abstract

To investigate the behavior of nucleolin and AgNORs proteins in ultra-violet ray (UV)-induced oral cancer apoptotic cells. Dynamic alteration of nucleolin and AgNORs proteins in UV-induced SCC-25 and HSG cells were examined using Western blot analysis and histocytochemistry methods. Dynamic alteration of nucleolin and AgNORs proteins are observed. Nucleolin disappeared or diffusely spread out into nucleus in apoptotic body of oral cancer cells. This results indicate that alteration of nucleolin is associated with the induction of DNA fragmentation or the final active phase of apoptosis induced by UV in oral cancer cells.

Research Products

• [Journal Article] 歯科用インプラントの術前X線CT検査に対する歯科放射線科の役割. (2009)
  • Author(s): 鬼頭慎司, 若杉(佐藤)奈緒, 小田昌史, 松本(武田)忍, 田中達朗, 小薗孝彰, 森本泰宏
  • Journal Title: 日口診誌 22 Pages: 157-64
  • NAID: 10026398796
  • Peer Reviewed
• [Journal Article] 歯学部5年生の研究室配属による歯科放射線ティーチングファイルの作成. (2009)
  • Author(s): 鬼頭慎司, 若杉(佐藤)奈緒, 田中達朗, 小田昌史, 松本(武田)忍, 深井康弘, 時津高俊, 森本泰宏
  • Journal Title: 歯放 49 Pages: 41-7
  • NAID: 10027301020
• [Journal Article] Relationship between the curative effects of carbamazepine administration and the neurovascular compression volume of the trigeminal nerve measured using magnetic resonance cisternography. (2009)
  • Author(s): Tanaka T, Sakamoto E, Shiiba S, Oda M, Kito S, Wakasugi-Sato N, Matsumoto-Takeda S, Imamura Y, Nakanishi O, Morimoto Y
  • Journal Title: Clin J Pain 25 Pages: 752-9
• [Journal Article] Importance of clinical features and CT findings in numb chin syndrome: report of two cases. (2009)
  • Author(s): Yoshioka I, Shiiba S, Tanaka T, Nishikawa T, Sakamoto E, Kito S, Oda M, Wakasugi-Sato N, Matsumoto-Takeda S, Kagawa S, Nakanishi O, Tominaga K, Morimoto Y
  • Journal Title: J Am Dent Assoc 140 Pages: 550-4
• [Journal Article] Double contour-like structures", characteristic magnetic resonance signals on mandibular condyles seen during child growth. (2009)
  • Author(s): Tanaka T, Konoo T, Yoshioka I, Habu M, Kito S, Kodama M, Oda M, Wakasugi-Sato N, Matsumoto S, Kagawa S, Terashita M, Tominaga K, Morimoto Y
  • Journal Title: In RESEARCH ADVANCES IN ORAL DISEASES 1(GLOBAL RESEARCH NETWORK, INDIA) Pages: 15-22
• [Journal Article] 歯科用インプラントの術前X線CT検査に対する歯科放射線科の役割 (2009)
  • Author(s): 鬼頭慎司, et al
  • Journal Title: 日口診誌 22 Pages: 157-164
  • NAID: 10026398796
  • Peer Reviewed
• [Journal Article] 歯学部5年生の研究室配属による歯科放射線ティーチングファイルの作成 (2009)
  • Author(s): 鬼頭慎司, et al
  • Journal Title: 歯放 49 Pages: 41-47
  • NAID: 10027301020
  • Peer Reviewed
• [Journal Article] Relationship between the curative effects of carbamazepine administration and the neurovascular compression volume of the trigeminal nerve measured using magnetic resonance cisternography. (2009)
  • Author(s): Tanaka T, et al
  • Journal Title: Clin J Pain 25 Pages: 752-759
  • Peer Reviewed
• [Journal Article] Dynamic magnetic resonance sialography for patients with xerostomia. (2008)
  • Author(s): Tanaka T, Ono K, Ansai T, Yoshioka I, Habu M, Tomoyose T, Yamashita Y, Nishida I, Oda M, Kuroiwa H, Wakasugi-Sato N, Okabe S, Kito S, Takahashi T, Tominaga K, Inenaga K, Morimoto Y
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106(1) Pages: 115-23
• [Journal Article] 顎口腔領域の膿瘍形成部同定におけるFASE拡散強調画像の有用性. (2008)
  • Author(s): 鬼頭慎司, 田中達朗, 若杉奈緒, 岡部幸子, 森本泰宏
  • Journal Title: 北九医工誌 18 Pages: 39-42
• [Journal Article] Assessment of the relationship between the mandibular cortex on panoramic radiographs and the risk of bone fracture and vascular disease in 80-year-olds. (2008)
  • Author(s): Okabe S, Morimoto Y, Ansai T, Yoshioka I, Tanaka T, Taguchi A, Kito S, Wakasugi-Sato N, Oda M, Kuroiwa H, Ohba T, Awano S, Takata Y, Takehara T
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106(3) Pages: 433-42
• [Journal Article] Usefulness of CT scan images for planning the bone cut for intra-oral vertical ramus osteotomy. (2008)
  • Author(s): Yoshioka I, Tanaka T, Oda M, Kodama M, Habu M, Kito S, Okabe S, Wakasugi-Sato N, Kuroiwa H, Tominaga K, Morimoto Y
  • Journal Title: J Maxillofac Oral Surg 7 Pages: 245-250
• [Journal Article] Usefulness of CT scan images for planning the bone cut for intra oral vertical ramus oste otomy (2008)
  • Author(s): Yoshioka I, Tanaka T, Oda M, Kodama M, Habu M, Kito S, Okabe S, Wakasugi-Sato N, Kuroiwa H, Tominaga K, Morimoto Y
  • Journal Title: J Maxillofac Oral Surg. 7 Pages: 245-250
  • Peer Reviewed
• [Journal Article] Assessment of the relationship between the mandibular cortex on panoramic radiographs an d the risk of fractures and vascular diseases in 80year-olds (2008)
  • Author(s): Okabe S, Morimoto Y, Ansai T, Yoshioka I, Tanaka T, Taguchi A, Kito S, Wakasu gi-Sato N, Oda M, Kuroiwa H, Ohba T, Awano S, Takata Y, Takehara T
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106 Pages: 433-42
  • Peer Reviewed
• [Journal Article] Dynamic MR sialography for patients with xerostomia (2008)
  • Author(s): Tanaka T, Ono K, Ansai T, Yoshioka I, Habu M, Tomoyose T, Yamashita Y, Nis hida I, Oda M, Kuroiwa H, Wakasugi-Sato N, Okabe S, Kito S Takahashi T, Tominaga K, Inenaga K, Morimoto Y
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106 Pages: 939-47
  • Peer Reviewed
• [Journal Article] 顎口腔領域の膿?形成部同定におけるFASE拡散強調画像の有用性 (2008)
  • Author(s): 鬼頭慎司, 田中達朗, 若杉奈緒, 岡部幸子, 森本泰宏
  • Journal Title: 北九医工誌 18 Pages: 39-42
  • Peer Reviewed
• [Journal Article] Functional evaluations of the parotid and submandibular glands using dynamic MR sialography. (2007)
  • Author(s): Tanaka T, Ono K, Habu M, Inoue H, Tominaga K, Okabe S, Kito S, Yokota M, Fukuda J, Inenaga K, Morimoto Y
  • Journal Title: Dentomaxillofac Radiol 36 Pages: 218-223
• [Journal Article] Functional evaluations of the parotid and submandibular glandsusing dynamic MR sialography. (2007)
  • Author(s): Tanaka T, Ono K, Habu M, Inoue H, Tominaga K, OkabeS, Kito S, Yokota M, FukudaJ, Inenaga K, Morimoto Y
  • Journal Title: Dentomaxillofac Radiol. 36 Pages: 218-223
  • Peer Reviewed
• [Presentation] FDG高集積を認め診断に苦慮したアミロイド沈着を伴う下顎骨骨髄炎の一例. (2009)
  • Author(s): 鬼頭慎司
  • Organizer: 日本歯科放射線学会第29回関西・九州合同地方会
  • Place of Presentation: 岡山大学
  • Year and Date: 2009-11-28
• [Presentation] FDG高集積を認め診断に苦慮したアミロイト沈着を伴う下顎骨骨髄炎の一例 (2009)
  • Author(s): 鬼頭慎司, et al
  • Organizer: 日本歯科放射線学会第29回関西・九州合同地方会
  • Place of Presentation: 岡山大学(岡山)
  • Year and Date: 2009-11-28
• [Presentation] 80才の高齢者ではパノラマエックス線画像上の下顎骨下縁皮質骨所見と骨折あるいは血管疾患リスクとの間に関連はあるのか? (2009)
  • Author(s): 鬼頭慎司
  • Organizer: 第50回日本歯科放射線学会総会・学術大会
  • Place of Presentation: 大阪
• [Presentation] 80才の高齢者ではパノラマエックス線画像上の下顎骨下縁皮質骨所見と骨折あるいは血管疾患リスクとの間に関連はあるのか? (2009)
  • Author(s): 鬼頭慎司, et al
  • Organizer: 第50回日本歯科放射線学会総会・学術大会
  • Place of Presentation: 大阪国際会議場(大阪)
• [Presentation] インプラント術前エックス線CT検査における画像診断のあり方について (2009)
  • Author(s): 麹若杉奈緒, 小田昌史, 松本忍, 田中達朗, 森本泰宏
  • Organizer: 日本歯科放射線学会第28回関西・九州合同地方会
  • Place of Presentation: 鹿児島市
• [Presentation] Correlation between the incidence of central nodal necrosis in neck node metastasis and the degree of differentiation in oral squamous cell carcinoma. (2008)
  • Author(s): S Kito, A Uchida, T Tanaka, N Wakasugi-Sato, S Matsumoto-Takeda, M Oda, T Hiromatsu1), K Harada2), K Hori3), I Yoshioka, Y Morimoto
  • Organizer: The 7th Asian Congress of Oral and Maxillofacial Radiology and The 13th Conference of Clinical Diagnostics of JSOMR
  • Place of Presentation: Nara, Japan
• [Presentation] インプラント術前エックス線CT検査における歯科放射線科の対応-トラブル症例より学んだことと病診連携の方向性に関する考察-. (2008)
  • Author(s): 鬼頭慎司
  • Organizer: 第21回日本口腔診断学会・第18回日本口腔粘膜学会共催学術集会
  • Place of Presentation: 東京
• [Presentation] 典型的所見を呈したGarre骨髄炎の1症例. (2008)
  • Author(s): 鬼頭慎司
  • Organizer: 日本歯科放射線学会第5回総会・第49回学術大会
  • Place of Presentation: 名古屋
• [Presentation] Correlation between the incidence of central nodal necrosis in neck node metastasis and the degree of differentiatin in oral squamous cell carcinoma (2008)
  • Author(s): Kito S Uchida A, Tanaka T, Wakasugi-S ato N, Matumoto S, Oda M, Hiromatsu T, Harada K, Hori K, Yoshioka I, Morim oto Y
  • Organizer: The 7th Asian Congress of Oral and Maxillofacial Radiology
  • Place of Presentation: 奈良市
• [Presentation] 典型的所見を呈したGarre骨髄炎の1症例 (2008)
  • Author(s): 鬼頭慎司, 田中達朗, 岡部幸子, 若杉奈緒, 小田昌史, 森本泰宏
  • Organizer: 日本歯科放射線学会第5回総会・第49回学術大会
  • Place of Presentation: 名古屋市
• [Presentation] インプラント術前エックス線CT検査における歯科放射線科の対応-トラブル症例より学んだことと病診連携の方向性に関する考察- (2008)
  • Author(s): 鬼頭慎司, 田中達朗, 若杉奈緒, 松本忍, 小田昌史, 森本泰宏
  • Organizer: 第21回日本口腔診断学会・第18回日本口腔粘膜学会共催学術1集会
  • Place of Presentation: 東京
• [Presentation] 下顎骨骨髄炎の波及により生じた化膿性顎関節炎の一症例 (2007)
  • Author(s): 鬼頭 慎司, 田中 達朗, 岡部 幸子, 若杉 奈緒, 小田 昌史, 森本 泰宏
  • Organizer: 日本歯科放射線学会第50回関西・第46回九州合同地方会
  • Place of Presentation: 大阪市
  • Year and Date: 2007-12-01
• [Presentation] 顎腔領域の膿瘍形成部同定におけるFASE拡散強調画像の有用性. (2007)
  • Author(s): 鬼頭 慎司, 田中 達朗, 岡部 幸子, 若杉 奈緒, 森本 泰宏
  • Organizer: 第39回北九州医工学術者会議
  • Place of Presentation: 北九州市
  • Year and Date: 2007-11-02
• [Presentation] 腔顎顔面領域の膿瘍形成部検出におけるFASE拡散強調画像の有用性. (2007)
  • Author(s): 鬼頭 慎司, 田中 達朗, 岡部 幸子, 森本 泰宏
  • Organizer: 第67回九州歯科学会総会
  • Place of Presentation: 北九州市
• [Presentation] Functional evaluation of parotid glands using dynamic MR sialography with citric acid stimulation. (2007)
  • Author(s): Morimoto Y, Okabe S, Yoshioka I, Ono K, Tanaka T, KitoS, Inenaga K
  • Organizer: The 16th International Congress of Dentoma xillofacial Radiology
  • Place of Presentation: Beijing,China
• [Presentation] Alteration of the magnetic resonance imaging signals characteristic of mandibular condyles in growing children. (2007)
  • Author(s): Tanaka T Okabe S, YoshiokaI, Tominaga K, Konoo T, Kito S, Morimoto Y
  • Organizer: The 16th International Congress of Dentoma xillofacial Radiology
  • Place of Presentation: Beijing,China
• [Book] New diagnostic imaging modalities for oral cancers (Chapter 5). In Oral Cancer Research Advances. (2007)
  • Author(s): Morimoto Y, Tanaka T, Yoshioka I, Yamashita Y, Hirashima S, Kodama M, Ariyoshi W, Tomoyose T, Furuta N, Habu M, Okabe S, Kito S, Oda M, Kuroiwa H, Wakasugi-Sato N, Takahashi T, Tominaga K
  • Publisher: Nova Science Publishers