New target therapy with OSCC -specific Endothelin inhibitor

• Project/Area Number: 19791387
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nagoya City University
• Principal Investigator: IMAI Masaki Nagoya City University, 大学院・医学研究科, 講師 (30440936)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: 口腔癌 / エンドセリン

Research Abstract

エンドセリン受容体阻害ペプチドは乳ガン細胞SKBR3, MCF7細胞において60-70%の増殖抑制がみられたが、口腔癌では増殖阻害活性を示さなかった。これらの結果より、口腔癌の増殖においてエンドセリンA受容体の機能は重要でない可能性が示唆された。また、口腔癌特異的な抗原(MUC1)に対する特異抗体C595 の単独での作用を調べた。C595 は補体依存性細胞障害反応、抗体依存性細胞障害反応をわずかに誘導したが、抗体単独では細胞増殖抑制には影響がなかった。従って、MUC1特異的モノクローナル抗体は口腔癌の標的化には有効だが、抗体単独での抗体免疫療法では有効性が見られないことが明らかになった。

Research Products

• [Journal Article] Effect of the antibody immunotherapy by the anti-MUC1 monoclonal antibody to the oral squamous cell carcinoma in vitro. (2008)
  • Author(s): Shimizu M, Imai M
  • Journal Title: Biol Pharm Bull. 31 Pages: 2288-2293
  • NAID: 110007005031
  • Peer Reviewed
• [Journal Article] Modulation of protective T cell immunity by complement inhibitor expression on tumor cells. (2008)
  • Author(s): Varela JC, Imai M, Atkinson C, Ohta R, Rapisardo M, Tomlinson S
  • Journal Title: Cancer Res. 68 Pages: 6734-6742
  • Peer Reviewed
• [Journal Article] Effects of active and inactive phospholipase D2 on signal transduction, adhesion, migration, invasion, and metastasis in EL4 lymphoma cells. (2008)
  • Author(s): Knoepp SM, Chahal MS, Xie Y, Zhang Z, Brauner DJ, Hallman MA, Robinson SA, Han S, Imai M, Tomlinson S, Meier KE.
  • Journal Title: Mol Pharmacol. 74 Pages: 574-584
  • Peer Reviewed
• [Journal Article] Designing complementary peptides with a genetic algorithm to a 10 amino acid peptide of the C-terminal of C5a anaphylatoxin. (2008)
  • Author(s): Hau L, Campbell W, Okada H, Del Carpio CA, Imai M , Okada N.
  • Journal Title: Nagoya Med. J. 49 Pages: 219-233
  • Peer Reviewed
• [Journal Article] 相補性ペプチドによる炎症の制御 (2008)
  • Author(s): 今井優樹,岡田則子
  • Journal Title: 臨床検査 52 Pages: 917-920
• [Journal Article] Effect of the antibody immunotherapy by the anti-MUC1 monoclonal an tibody to the oral squamous cell carcinoma in vitro (2008)
  • Author(s): Shimizu M
  • Journal Title: Biological & Pharmaceutical Bulletin 31 Pages: 2288-2293
  • Peer Reviewed
• [Journal Article] Modulation of protective T cell immunity by complement inhibitor expre ssion on tumor cells (2008)
  • Author(s): Varela JC
  • Journal Title: Cancer Research 68 Pages: 6734-6742
  • Peer Reviewed
• [Journal Article] Effects of active and inactive phospholipase D2 on signal transduction, adhesion, migration, invasion, and metastasis in EL4 lymphoma cells (2008)
  • Author(s): Knoepp SM
  • Journal Title: Molecular Pharmacology 74 Pages: 574-584
  • Peer Reviewed
• [Journal Article] Designing complementary peptides with a genetic algorithm to a 10 amino acid peptide of the C-terminal of C5a anaphylatoxin (2008)
  • Author(s): Hau, Lewis
  • Journal Title: Nagoya Medical Journal (In press)
  • Peer Reviewed
• [Journal Article] Enhancement of Antibody-Dependent Mechanisms of Tumor Cell Lysis by a Targeted Activator of Complement. (2007)
  • Author(s): Imai M , Ohta R, Varela JC, Song H, Tomlinson S.
  • Journal Title: Cancer Res. 67 Pages: 9535-9541
  • Peer Reviewed
• [Journal Article] Inhibition of HIV-1 infection by synthetic peptides derived CCR5 fragments. (2007)
  • Author(s): Imai M, Baranyi L, Okada N, Okada H.
  • Journal Title: Biochem. Biophys. Res. Commun. 353 Pages: 851-856
  • Peer Reviewed
• [Journal Article] Enhancement of Antibody-Dependent Mechanisms of Tumor Cell Lysis by a Targeted Activator of Complement (2007)
  • Author(s): Imai, Masaki
  • Journal Title: Cancer Research 67 Pages: 9535-9541
  • Peer Reviewed
• [Journal Article] Inhibition of HIV-1 infection by synthetic peptides derived CCR5 fragm ents (2007)
  • Author(s): Imai, Masaki
  • Journal Title: Biochem.Biophys.Res.Commun 353 Pages: 851-856
  • Peer Reviewed
• [Presentation] Immune enhancement in MUC1 transgenic mice immunized with MUC1-C3d fusion protein. (2008)
  • Author(s): Imai M, Ohta R, He C, Okada N, Ross T, Tomlinson S.
  • Organizer: 第38回日本免疫学会総会・学術集会要旨集
  • Place of Presentation: 京都
• [Presentation] ヒト抗Nef-IgM抗体はHIV-1感染拡大を阻止する. (2008)
  • Author(s): Okada N, Tobisawa S, Imai M, Okada H.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] Effect of the antibody immunotherapy by the anti-MUC1 monoclonal antibody to the oral squamous cell carcinoma. (2008)
  • Author(s): Imai M.
  • Organizer: XXII International Complement Workshop, Basel
  • Place of Presentation: Switzerland
• [Presentation] Antibody immunotherapy against OSCC. (2008)
  • Author(s): Shimizu M, Imai M, Iseki T, Morita S.
  • Organizer: XIX Congress of the European Association for Cranio-Maxillo-Facial Surgery, Bologna
  • Place of Presentation: Italy
• [Presentation] MUC1-C3dワクチンによる免疫増強効果、自然免疫の最前線 (2008)
  • Author(s): 太田里永子,Chun He, Ted Ross,岡田則子,Stephen Tomlinson,今井優樹.
  • Organizer: 第73回日本インターフェロン・サイトカイン学会学術集会・第19回日本生体防御学会学術総会・第45回補体シンポジウム合同大会
  • Place of Presentation: 札幌
• [Presentation] 腫瘍特異抗原MUC1を標的とする口腔癌の抗体免疫療法の基礎的研究-補体膜制御因子との関連性について- (2008)
  • Author(s): 清水ももこ,今井優樹,井関富雄,森田章介.
  • Organizer: 第32回日本頭頸部癌学会
  • Place of Presentation: 東京
• [Presentation] 腫瘍特異抗原MUC1を標的とする口腔癌の抗体免疫療法の研究 (2008)
  • Author(s): 清水ももこ,今井優樹,井関富雄,森田章介.
  • Organizer: 第62回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 口腔癌に対する抗MUC1モノクローナル抗体の抗腫瘍活性の解析 (2008)
  • Author(s): 今井優樹
  • Organizer: 第128回日本薬学会
  • Place of Presentation: 横浜
• [Presentation] 腫瘍特異抗原MUC1を標的とした口腔癌の新規抗体免疫療法の開発 (2008)
  • Author(s): 清水ももこ,今井優樹,井関富雄,森田章介.
  • Organizer: 第26回日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 大分
• [Presentation] Effect of the antibody immunotherapy by the anti-MUC1 monoclonal an tibodv to the oral sauamous cell carcinoma (2008)
  • Author(s): Imai M
  • Organizer: XXII International Complement Workshop
  • Place of Presentation: Basel, Switzerland
• [Presentation] Antibody immunotherapy against OSCC (2008)
  • Author(s): Shimizu M
  • Organizer: XIX Congress of the European Association for Cr anio-Maxillo-Facial Surgery
  • Place of Presentation: Bologna, Italy
• [Presentation] Immune enhancement in MUC1 transgenic mice immunized with MUC1 -C3d fusion protein (2008)
  • Author(s): Imai M
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] ヒト抗NeflgM抗体はHIV1感染拡大を阻止する (2008)
  • Author(s): Okada N
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] MUC1-C3dワクチンによる免疫増強効果 (2008)
  • Author(s): 太田里永子
  • Organizer: 自然免疫の最前線(第73回日本インターフェロン・サイトカイン学会学術集会・第19回日本生体防御学会学術総会・第45回補体シンポジウム合同大会)
  • Place of Presentation: 札幌
• [Presentation] 腫瘍特異抗原MUC1を標的とする口腔癌の抗体免疫療法の基礎的研究-補体膜1制御因子との関連性について- (2008)
  • Author(s): 清水ももこ
  • Organizer: 第32回日本頭頸部癌学会
  • Place of Presentation: 東京
• [Presentation] 口腔癌に対する抗MUC1モノクローナル抗体の抗腫瘍活性の解析 (2008)
  • Author(s): 今井 優樹
  • Organizer: 第128回日本薬学会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
• [Presentation] 腫瘍特異抗原MUC1を標的とした口腔癌の新規抗体免疫療法の開発 (2008)
  • Author(s): 清水 ももこ
  • Organizer: 第26回日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 大分県別府国際コンベンションセンター