Analysis of thermo-sensitive nociceptor expression and mechanism by using dentin-pulp complex culture system.

• Project/Area Number: 19791404
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Kagoshima University
• Principal Investigator: TATSUYAMA Shoko Kagoshima University, 医学部・歯学部附属病院, 助教 (70347095)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥5,010,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥810,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 象牙芽歯髄複合体 / 温度感受性受容体 / 象牙芽細胞 / 歯髄細胞 / VR-1 / TRPV8 / 温度感受性イオンチャンネル / 象牙質歯髄複合体 / 象牙知覚過敏症 / 温感受性イオンチャンネル / 免疫染色法 / 象牙質知覚過敏症

Research Abstract

In this study, we confirmed the expression of the transient receptor potential melastatin-8 (TRPM8) in human dental pulp cells and in mouse odontoblasts. Using rat dental pulp slice culture as a dentin-pulp complex models we further revealed TRPM8 expression in rat odontoblasts. Our results indicated that odontoblasts could directly respond to noxious stimuli, example as cold condition, and transduce the signal to the nearby nerves, accordingly odontblasts may be involved in dentin hypersensitivity.

Research Products

• [Presentation] 歯髄および象牙芽細胞における冷刺激受容体TRPM8発現の検討 (2007)
  • Author(s): 達山祥子, 徳田雅行, 川上克子, 作田哲也, 小山徹, 梶原武弘, 長岡成孝, 鳥居光男
  • Organizer: 日本歯科保存学会春季大会
  • Place of Presentation: 埼玉県さいたま市
  • Year and Date: 2007-06-07
• [Presentation] 歯髄および象牙芽細胞における冷刺激受容体TRPM8発現の検討 (2007)
  • Author(s): 達山 祥子
  • Organizer: 日本歯科保存学会2007年度春季学術大会(126回)
  • Place of Presentation: 埼玉県大宮市
  • Year and Date: 2007-06-07