Establishment of the bone induction system by immature muscular tissue

• Project/Area Number: 19791472
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 補綴理工系歯学
• Research Institution: Aichi Gakuin University
• Principal Investigator: HAYASHI Tatsuhide Aichi Gakuin University, 歯学部, 講師 (70367621)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,390,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 歯科用材料・歯科理工学 / BMP / 未成熟筋組織 / 骨組織誘導 / 骨誘導 / マイクロX線CT解析 / CaCl_2 / e-PTFE / in vitro / 骨様組織 / 石灰化

Research Abstract

胎仔ラットの未成熟筋組織に骨形成因子であるBMPを作用させ,e-PTFE膜状で培養したところ,培養2週間後に骨様組織が誘導されていた(H-E染色).さらにこの培養組織が骨系の組織であることを組織(免疫染色),遺伝子,元素レベルで解析し,証明することができた.

Research Products

• [Journal Article] Histological analysis of induced cartilage on the biodegradable or non-biodegradable membranes from immature muscular tissue in vitro (2008)
  • Author(s): T. Hayashi, T. Kawai, A. Ishikawa, H. Kawai, K. Nakano, Y. Takei. K. Kuroki
  • Journal Title: Journal of biomedical material research part A 15 ; 86(4) Pages: 1048-1054
  • Peer Reviewed
• [Journal Article] Histological analysis of induced cartilage on the biodegradable or non-biodegradable membranes from immature muscular tissue in vitro (2008)
  • Author(s): T. Hayashi, T. Kawai, A. Ishikawa, H. Kawai, K. Nakano, Y. Takei, K. Kuroki
  • Journal Title: Journal of biomedical material research part A (In press)
  • Peer Reviewed
• [Journal Article] Histological analysis of induced cartilage on the biodegradable or nonbiodegradable membranes from immature muscular tissue in vitro
  • Author(s): T. Hayashi, T. Kawai, A. Ishikawa, H. Kawai, K. Nakano, Y. Takei, K. Kuroki
  • Journal Title: J Biomed Mater Res A 15;86(4) Pages: 1048-1054
• [Presentation] Aiming for ossification in vitro-Various Analyses of Induced Bone-Like Tissue in vitro-Academy of Prosthetic and Regenerative Sciences (2008)
  • Author(s): T. Hayashi, Y. Sato, T. Asai, S. Hamajima, M. Asakura, T. Kiriyama, T. Kawai
  • Place of Presentation: Nagoya
  • Year and Date: 2008-11-28
• [Presentation] Aiming for ossification in vitro - Various Analyses of Induced Bone-Like Tissue in vitro - (2008)
  • Author(s): T. Hayashi, Y. Sato, T. Asai, S. Hamajima, M. Asakura, T. Kiriyama, T. Kawai
  • Organizer: Academy of Prosthetic and Regenerative Sciences
  • Place of Presentation: Nagoya
  • Year and Date: 2008-11-28
• [Presentation] Ca2+ promotes the calcification of induced bone-like tissue in vitro (2008)
  • Author(s): T. Hayashi, T. Kawai, T. Asai, M. Okano, Y. Sato, M. Asakura
  • Organizer: 86th General Session & Exibition of the IADR
  • Place of Presentation: Toronto
  • Year and Date: 2008-07-04
• [Presentation] Ca^<2+> promotes the calcification of induced bone-like tissue in vitro (2008)
  • Author(s): T. Hayashi, T. Kawai, T. Asai, M. Okano. Y. Sato. M. Asakura
  • Organizer: 86^<th> General Session & Exibition of the IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-04
• [Presentation] The influence of Ca2+ on bone-like tissue induction in vitro (2007)
  • Author(s): T. Hayashi, T. Kawai, K. Kuroki, T. Asai, M. Okano, M. Sato, M. Taniyama
  • Organizer: International Dental Materials Congress 2007
  • Place of Presentation: Bangkok
  • Year and Date: 2007-11-24
• [Presentation] The influence of the Ca2+on bone-like tissue induction in vitro (2007)
  • Author(s): T. Hayashi, K. Kuroki, T. Asai, M. Okano, H. Kawai and T. Kawai
  • Organizer: International Dental Materials Congress 2007
  • Place of Presentation: Bangkok, Thai
  • Year and Date: 2007-11-24
• [Presentation] 誘導骨様組織による骨組織再生の試み (2007)
  • Author(s): 林達秀,河合逹志,黒木健次郎,浅井崇文,岡野正史,塚脇篤也,藤本耕太郎
  • Organizer: 第49回日本歯科理工学会学術講演会
  • Place of Presentation: 札幌
  • Year and Date: 2007-05-13
• [Presentation] 誘導骨様組織による骨組織再生の試み. (2007)
  • Author(s): 林 達秀, 河合逹志, 黒木健次郎, 浅井崇文, 岡野正史, 塚脇篤也, 藤本耕太郎.
  • Organizer: 第49回日本歯科理工学会学術講演会
  • Place of Presentation: 札幌
  • Year and Date: 2007-05-13