The development of a novel screening method for cancer metastasis

• Project/Area Number: 19791516
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: MURAKAMI Jyun Okayama University, 大学院・医歯薬学総合研究科, 助教 (40362983)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,910,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 実験腫瘍学 / 臨床 / 薬理 / 発現制御 / 遺伝子 / 薬理学

Research Abstract

癌幹細胞を多数有する、もしくは幹細胞的機能に富むと考えられる高転移腫瘍を樹立、それらの細胞株を対象に、網羅的なPCRアレイにて、転移経路ならびに血管新生経路に関与する遺伝子についてRNA発現比較した。いくつかの遺伝子群の発現差を認め、転移能、腫瘍形成能にとって重要な因子を絞り込むことが出来た。今後は、それらの候補遺伝子を指標に癌幹細胞のスクリーニングを展開していきたいと考えている。

Research Products

• [Journal Article] Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis (2009)
  • Author(s): Ekuni D, Tomofuji T, Sanbe T, Irie K, Azuma T, Maruyama T, Tamaki N, Murakami J, Kokeguchi S, Yamamoto T.
  • Journal Title: J Periodontal Res (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Effects of vitamin C intake on gingival oxidative stress in rat periodontitis (2009)
  • Author(s): Tomofuji T, Ekuni D, Sanbe T, Irie K, Azuma T, Maruyama T, Tamaki N, Murakami J, Kokeguchi S, Yamamoto T.
  • Journal Title: Free Radic Biol Med 46(2) Pages: 163-8
  • Peer Reviewed
• [Journal Article] Effects of vitamin C intake on gingival oxidative stress in rat periodontitis (2009)
  • Author(s): Tomofuji T, Ekuni D, Sanbe T, Ir
  • Journal Title: Free Radic Biol Med. 46 Pages: 163-168
  • Peer Reviewed
• [Journal Article] Dynamic contrast-nhanced magnetic resonance imaging for estimating tumor proliferation and microvessel density of oral squamous cell carcinomas (2009)
  • Author(s): Unetsubo T, Konouchi H, Yanagi Y
  • Journal Title: Oral Oncol. (in press)
  • Peer Reviewed
• [Journal Article] Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis (2009)
  • Author(s): Ekuni D, Tomofuji T, Sanbe T, Ir
  • Journal Title: J Periodontal Res. (in press)
  • Peer Reviewed
• [Journal Article] A spindle cell carcinoma presenting with osseous metaplasia in the gingiva: a case report with immunohistochemical analysis (2008)
  • Author(s): Katase N, Tamamura R, Gunduz M, Murakami J, Asaumi J, Tsukamoto G, Sasaki A, Nagatsuka H.
  • Journal Title: Head Face Med 4 Pages: 28-28
  • Peer Reviewed
• [Journal Article] Dynamic contrast-enhanced magnetic resonance imaging for estimating tumor proliferation and microvessel density of oral squamous cell carcinomas (2008)
  • Author(s): Unetsubo T, Konouchi H, Yanagi Y, Murakami J, Fujii M, Matsuzaki H, Hisatomi M, Nagatsuka H, Asaumi JI.
  • Journal Title: Oral Oncol (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Recombinant human bone morphogenetic protein-2/atelocollagen composite as a new material for ossicular reconstruction (2008)
  • Author(s): Takeuchi A, Tsujigiwa H, Murakami J, Kawasaki A, Takeda Y, Fukushima K, Rodriguez AP, Nagatsuka H, Yamada M, Nishizaki K.
  • Journal Title: J Biomed Mater Res A (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Progress in Oral Cancer Research (2008)
  • Author(s): Jun Murakami, et. al.
  • Journal Title: Nova Science Publishers, Inc.NY Pages: 13-54
• [Journal Article] Microsatellite instability and clinicopathological features in esophageal squamous cell cancer (2007)
  • Author(s): Matsumoto Y, Nagasaka T, Kambara T, Hoshizima N, Murakami J, Sasamoto H, Hosokawa M, Naomoto Y, Isozaki H, Shimizu K, Tanaka N, Matsubara N
  • Journal Title: Oncol Rep 18(5) Pages: 1123-7
  • Peer Reviewed
• [Journal Article] Diagnostic value of dynamic contrast-enhanced MRI in the salivary gland tumors (2007)
  • Author(s): Hisatomi M, Asaumi J, Yanagi Y, Unetsubo T, Maki Y, Murakami J, Matsuzaki H, Honda Y, Konouchi H.
  • Journal Title: Oral Oncol 43(9) Pages: 940-7
  • Peer Reviewed
• [Journal Article] Depletion of O6-methylguanine-DNA methyltransferase by O6-benzylguanine enhances 5-FU cytotoxicity in colon and oral cancer cell lines (2007)
  • Author(s): Murakami J, Lee YJ, Kokeguchi S, Tsujigiwa H, Asaumi J, Nagatsuka H, Fukui K, Kuroda M, Tanaka N, Matsubara N
  • Journal Title: Oncol Rep 17(6) Pages: 1464-7
  • Peer Reviewed
• [Journal Article] Depletion of 06-methylguanine-DNA methyltransferase by 06-benzylguanine enhances 5-FU cytotoxicity in colon and oral cancer cell lines. (2007)
  • Author(s): Murakami J, et. al.
  • Journal Title: Oncol Rep. 17 Pages: 1461-1467
  • Peer Reviewed
• [Presentation] 大腸鋸歯状polyp及び腺管腺腫におけるメチル化、K-ras, B-raf遺伝子、MSIの解析と比較 (2008)
  • Author(s): 長尾厚樹、松原長秀、神原健、能登原憲司 笹本博美、村上純、内田卓之、星島直子、田中紀章
  • Organizer: 第63回日本消化器外科学会総会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2008-07-17
• [Presentation] Effects of Obesity on Oxidative Stress in Rat Gingiva (2008)
  • Author(s): T. TOMOFUJI, T. SANBE, N. TAMAKI, D. EKUNI, K. KASUYAMA, M. UMAKOSHI, K. IRIE, T. AZUMA, J. MURAKAMI, S. KOKEGUCHI, T. YAMAMOTO and M. MORITA
  • Organizer: The 56th Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: Japan
• [Presentation] A novel benzylidene lactam compound, KNK437 inhibits the invasion and angiogenesis in oral cancer cell lines (2008)
  • Author(s): J Murakami, J Asaumi
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 口腔癌細胞株に対する新規抗癌剤KNK437による血管新生阻害と癌浸潤阻害作用について (2008)
  • Author(s): 村上純
  • Organizer: 日本歯科放射線学会第49回・総会・学術大会
  • Place of Presentation: 名古屋国際会議場
• [Book] Inc Progress in Oral Cancer Research Recent Progress in Molecular Targets and Oncology Therapeutics (2008)
  • Author(s): Jun Murakami, Jun-ichi Asaumi, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Masahiro Kuroda, You-Jin Lee and Susumu Kokeguchi
  • Publisher: Nova Science Publishers
• [Book] Progress in Oral Canceer Research Recent Progress in Molecular Targets and Oncology Therapeutice (2008)
  • Author(s): Jun Murakami, Jun-ichi Asaumi, Hide
  • Total Pages: 44
  • Publisher: Nova Science Publishers, Inc
• [Book] Progress in Oral Cancer Research (2008)
  • Author(s): Jun Murakami, et. al.
  • Total Pages: 197
  • Publisher: Nova Science Publishers, Inc.NY