Combination chemotherapy for head and neck squamous cell carcinoma

• Project/Area Number: 19791553
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: OZAWA Shigeyuki Kanagawa Dental College, 歯学, 特別研究員 (40434394)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,520,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 頭頸部扁平上皮癌 / CXCL14 / BRAK / テーラーメイド医療 / メチル化 / 癌 / ゲフィチニブ / アザシチジン / 分子標的治療 / マーカー分子

Research Abstract

我々はBRAKの遺伝子発現が確認できない癌細胞を、デシタビン処理するとBRAKの遺伝子発現が回復することを見出した。BRAKのプロモーター領域はメチル化によって不活化しており、ゲフィチニブ処理においても、BRAKの遺伝子発現回復による抗腫瘍効果は期待できない。BRAKのメチル化をマーカー分子として、(1)メチル化を受けていない場合はゲフィチニブ単剤、(2)メチル化を受けている場合はデシタビンとゲフィチニブの多剤併用療法が期待される。

Research Products

• [Journal Article] Acidic extracellular pH increases calcium influx-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma (2007)
  • Author(s): Kato Y, Ozawa S, Tsukuda M, Kubota E, Miyazaki K, St-Pierre Y, Hata R
  • Journal Title: FEBS J 274(12) Pages: 3171-83
• [Journal Article] Type III collagen is essential for growth acceleration of human osteoblastic cells by ascorbic acid 2-phosphate, a long-acting vitamin C derivative (2007)
  • Author(s): Maehata Y, Takamizawa S, Ozawa S, Izukuri K, Kato Y, Sato S, Lee MC, Kimura A, Hata R
  • Journal Title: Matrix Biol 26(5) Pages: 371-81
• [Presentation] CXCL14/BRAKの発現調節機構 (2008)
  • Author(s): 小澤重幸,加藤靖正,伊藤慎,小森令賀,鈴木健司,前畑洋次郎,李昌一,久保田英朗,畑隆一郎
  • Organizer: 第43回総会
  • Place of Presentation: 横須賀,神奈川
  • Year and Date: 2008-12-06
• [Presentation] Determination of transcription start-site of CXCL14/BRAK in squamous cell carcinoma (2008)
  • Author(s): Komori R., Ozawa S., Kato Y., Shinji H., Kimoto S., Hata R.
  • Organizer: International Association for Dental Research
  • Place of Presentation: Toronto. Canada
  • Year and Date: 2008-07-02
• [Presentation] p38 signaling up-regulates BRAK expression in squamous cell carcinoma (2008)
  • Author(s): Ozawa S., Kato Y., Ito S., Komori R., Maehata Y., Kubota E., Hata R.
  • Organizer: 86th General Session and Exhibition of the IADR・32nd Annual Meeting of the CADR
  • Place of Presentation: Toronto,Canada
• [Presentation] Type III collagen regulates growth of human osteoblastic cells (2008)
  • Author(s): Maehata Y., Takamizawa S., Ozawa S., Izukuri K., Kato Y., Sato S., Yoshino F., Lee M-C., Hata R.
  • Organizer: 86th General Session and Exhibition of the IADR・32nd Annual Meeting of the CADR
  • Place of Presentation: Toronto, Canada
• [Presentation] p38 signaling up-regulates BRAK expression in squamous cell carcinoma (2008)
  • Author(s): 小澤重幸
  • Organizer: IADR
  • Place of Presentation: Canada Toronto
• [Presentation] ケモカインBRAK/CXCL14を用いた頭頸部扁平上皮癌へのIressa(ZD1839)感受性の検討 (2007)
  • Author(s): 小澤重幸
  • Organizer: 第61回日本口腔科学会学術集会
  • Place of Presentation: 神戸
• [Presentation] ケモカインBRAK/CXCL14を用いた頭頸部扁平上皮癌へのIressa(ZD1839)感受性の検討 (2007)
  • Author(s): 小澤重幸
  • Organizer: KEYSTONE SYMPOSIA
  • Place of Presentation: Keystone Resort