Classification of virulence of S. mutans strains using molecular epidemiological approaches

• Project/Area Number: 19791572
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Osaka University
• Principal Investigator: NOMURA Ryota Osaka University, 歯学部附属病院, 医員 (90437385)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,570,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: Streptococcus mutans / う蝕 / 臨床分離株 / ハウスキーピング遺伝子 / MLST 法 / S. mutans / 分子疫学 / 系統樹 / ビルレンス / S.mutans

Research Abstract

Streptococcus mutans はう蝕の主要な原因細菌であるとともに感染性心内膜炎の起炎菌としても知られている.本研究では,国内外のう蝕および感染性心内膜炎患者血液より分離された S. mutans 菌株を用いて,分子疫学的解析法である Multi Locus SequenceTyping (MLST) 法を確立し,病原性や伝播に関する系統的分析を行った.その結果,病原性に関与する血清型や遺伝子を保有する菌株が,系統樹上のある特定の領域に集中することが明らかとなった.

Research Products

• [Journal Article] Molecular and clinical analyses of gene encoding collagen-binding adhesin of Streptococcus mutans (2009)
  • Author(s): Nomura R, Nakano K, Tanigichi N, Lapirattanakul J, Nemoto H, Gronroos L, Alaluusua S, Ooshima T
  • Journal Title: J Med Microbiol 58 Pages: 469-475
  • Peer Reviewed
• [Journal Article] Upregulation of S100 calcium-binding protein A9 is required for induction of human smooth muscle cell proliferation by a periodontal pathogen (2009)
  • Author(s): Inaba H, Hokamura K, Nakano K, Nomura R, Katayama K, Nakajima A, Yoshioka H, Taniguchi K, Kamisaki Y, Ooshima T, Umemura K, Murad F, Wada K, Amano A
  • Journal Title: FEBS Lett 583 Pages: 128-134
  • Peer Reviewed
• [Journal Article] Detection of oral bacteria in cardiovascular specimens (2009)
  • Author(s): Nakano K, Nemoto H, Nomura R, Inaba H, Yoshioka H, Taniguchi K, Amano A, Ooshima T
  • Journal Title: Oral Microbiol Immunol 24 Pages: 64-68
  • Peer Reviewed
• [Journal Article] Molecular analyses of bacterial DNA in extirpated heart valves from infective endocarditis patients (2009)
  • Author(s): Nomura R, Nakano K, Nemoto H, Mukai T, Hata H, Toda K, Yoshioka H, Taniguchi K, Amano A, Ooshima T
  • Journal Title: Oral Microbiol Immunol 24 Pages: 43-49
  • Peer Reviewed
• [Journal Article] Molecular analyses of bacterial DNA in extirpated heart valves from infective endocarditis patients (2009)
  • Author(s): Nomura R, Nakano K, Nemoto H, et.al.
  • Journal Title: Oral Microbiology and Immunology 24 Pages: 43-49
  • Peer Reviewed
• [Journal Article] Molecular and clinical analyses of gene encoding collagen-binding adhisin of Streptococcus mutans (2009)
  • Author(s): Nomura R, Nakano K, Taniguchi N, et.al.
  • Journal Title: Journal of Medical Microbiology (In press)
  • Peer Reviewed
• [Journal Article] Demonstration of mother to child transmission of Streptococcus mutans using multilocus sequence typing (2008)
  • Author(s): Lapirattanakul J, Nakano K, Nomura R, Hamada S, Nakagawa I, Ooshima T
  • Journal Title: Caries Res 42 Pages: 466-474
  • Peer Reviewed
• [Journal Article] Distribution of 10 periodontal bacterial species in children and adolescents over 7-year period (2008)
  • Author(s): Nakano K, Miyamoto E, Tamura K, Nemoto H, Fujita K, Nomura R, Ooshima T
  • Journal Title: Oral Dis 14 Pages: 658-664
  • Peer Reviewed
• [Journal Article] Protein antigen in serotype k Streptococcus mutans clinical isolates (2008)
  • Author(s): Nakano K, Nomura R, Nemoto H, Lapirattanakul J, Taniguchi N, Gronroos L, Alaluusua S, Ooshima T
  • Journal Title: J Dent Res 87 Pages: 964-968
  • Peer Reviewed
• [Journal Article] Molecular characterization of Streptococcus mutans strains isolated from heart valve of infective endocarditis patient (2008)
  • Author(s): Nemoto H, Nakano K, Nomura R, Ooshima T
  • Journal Title: J Med Microbiol 57 Pages: 891-895
  • Peer Reviewed
• [Journal Article] Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients (2008)
  • Author(s): Nakano K, Inaba H, Nomura R, Nemoto H, Takeuchi H, Yoshioka H, Toda K, Taniguchi K, Amano A, Ooshima T
  • Journal Title: Oral Microbiol Immunol 23 Pages: 170-172
  • Peer Reviewed
• [Journal Article] Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae (2008)
  • Author(s): Inaba H, Nakano K, Kato T, Nomura R, Kawai S, Kuboniwa M, Ishihara K, Ooshima T, Amano A
  • Journal Title: Oral Microbiol Immunol 23 Pages: 29-35
  • Peer Reviewed
• [Journal Article] Demonstration of mother to child transmission of Streptococcus mutans using multilocus sequence typing (2008)
  • Author(s): Lapirattanakul J, Nakano K, Nomura R, et.al.
  • Journal Title: Caries Research 42 Pages: 466-474
  • Peer Reviewed
• [Journal Article] Detection of novel serotype k Streptococcus mutans in infective endocarditis patients (2007)
  • Author(s): Nakano K, Nomura R, Nemoto H, Mukai T, Yoshioka H, Shudo Y, Hata H, Toda K, Taniguchi K, Amano A, Ooshima T
  • Journal Title: J Med Microbiol 56 Pages: 1413-1415
  • Peer Reviewed
• [Journal Article] Repeated bacteremia caused by Streptococcus mutans in patient complicated with Sjogren's syndrome (2007)
  • Author(s): Nomura R, Hamada M, Nakano K, Nemoto H, Fujimoto K, Ooshima T
  • Journal Title: J Med Microbiol 56 Pages: 988-992
  • Peer Reviewed
• [Journal Article] Streptococcus mutans exhibits clonal variation as revealed by multilocus sequence typing (2007)
  • Author(s): Nakano K, Lapirattanakul J, Nomura R, Nemoto H, Alaluusua S, Gronroos L, Vaara M, Hamada S, Ooshima T, Nakagawa I
  • Journal Title: J Clin Microbiol 45 Pages: 2616-2625
  • Peer Reviewed
• [Journal Article] Serotype distribution of Streptococcus mutans, a pathogen of dental caries, in cardiovascular specimens from Japanese patients (2007)
  • Author(s): Nakano K, Nemoto H, Nomura R, Homma H, Yoshioka H, Shudo Y, Hata H, Toda K, Taniguchi K, Amano A, Ooshima T
  • Journal Title: J Med Microbiol 56 Pages: 551-556
  • Peer Reviewed
• [Journal Article] Detection and serotype distribution of Actinobacillus actinomycetemcomitans in cardiovascular specimens from Japanese patients (2007)
  • Author(s): Nakano K, Inaba H, Nomura R, Nemoto H, Tamura K, Miyamoto E, Yoshioka H, Taniguchi K, Amano A, Ooshima T
  • Journal Title: Oral Microbiol Immunol 22 Pages: 136-139
  • Peer Reviewed
• [Journal Article] Streptococcus mvtans exhibits clonal variation as revealed by multilocus sequence typing. (2007)
  • Author(s): Nakano K, Lapirattanakul J, Nomura R, et. al.
  • Journal Title: Journal of Clinical Microbiology 45 Pages: 2616-2625
  • Peer Reviewed
• [Journal Article] Serotype distribution of Streptococcus mutons, a pathogen of dental caries,in cardiovascular specimens from Japanese patients. (2007)
  • Author(s): Nakano K, Nemoto H, Nomura R, et. al.
  • Journal Title: Journal of Medical Microbiology 56 Pages: 551-556
  • Peer Reviewed
• [Journal Article] Repeated bacteremia caused by Streptococcus mutans in patient complicated with Sjogren's syndrome. (2007)
  • Author(s): Nomura R, Hamada M, Nakano K, et. al.
  • Journal Title: Journal of Medical Microbiology 56 Pages: 988-992
  • Peer Reviewed
• [Journal Article] Detection of cariogenic Streptococcus mutans in extirpated heart valve and atheromatous plaque specimens (2006)
  • Author(s): Nakano K, Inaba H, Nomura R, Nemoto H, Takeda M, Yoshioka H, Matsue H, Takahashi T, Taniguchi K, Amano A, Ooshima T
  • Journal Title: J Clin Microbiol 44 Pages: 3313-3317
  • Peer Reviewed
• [Journal Article] Isolation and characterization of Streptococcus mutans in heart valve and dental plaque specimens from a patient with infective endocarditis (2006)
  • Author(s): Nomura R, Nakano K, Nemoto H, Fujita K, Inagaki S, Takahashi T, Taniguchi K, Takeda M, Yoshioka H, Amano A, Ooshima T
  • Journal Title: J Med Microbiol 55 Pages: 1135-1140
  • Peer Reviewed
• [Journal Article] Bacterial profiles of oral streptococcal and periodontal bacterial species in saliva specimens from Japanese subjects
  • Author(s): Miyamoto E, Nakano K, Fujita K, Nomura R, Okawa R, Matsumoto M, Ooshima T
  • Journal Title: Arch Oral Biol (in press)
  • Peer Reviewed
• [Journal Article] Contribution of cell surface protein antigen Pac of Streptococcus mutans to platelet aggregation
  • Author(s): Matsumoto-Nakano M, Tsuji M, Inagaki S, Fujita K, Nagayama K, Nomura R, Ooshima T
  • Journal Title: Oral Microbiol Immunol (in press)
  • Peer Reviewed
• [Presentation] P.gingivalis 感染による血管内皮肥厚メカニズムの解明 (2009)
  • Author(s): 野村良太, 仲野和彦, 大嶋 隆
  • Organizer: 第47 回日本小児歯科学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2009-05-15
• [Presentation] Streptococcus mutans 臨床分離株におけるコラーゲン結合能に関する分析 (2008)
  • Author(s): 谷口奈穂,野村良太,仲野和彦,大嶋 隆
  • Organizer: 第27 回日本小児歯科学会近畿地方会大会
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-19
• [Presentation] 心臓血管疾患病変部組織において検出されるStreptococcus mutans の分子生物学的解析 (2008)
  • Author(s): 根本浩利,仲野和彦,野村良太,大嶋 隆
  • Organizer: 第27 回日本小児歯科学会近畿地方会大会
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-19
• [Presentation] Clinical analysis of gene encoding collagen-binding adhesin of Streptococcus mutans (2008)
  • Author(s): Nomura R, Nakano K, Taniguchi N, Lapirattanakul J, Nemoto H, Ooshima T
  • Organizer: 86th International Association of Dental Research
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] 唾液サンプル中のS. mutans cnm 遺伝子の保有と関連する臨床所見の検討 (2008)
  • Author(s): 谷口奈穂,野村良太,仲野和彦,大嶋 隆
  • Organizer: 第46 回日本小児歯科学会大会
  • Place of Presentation: さいたま
  • Year and Date: 2008-06-12
• [Presentation] 感染性心内膜炎患者心臓弁に存在する口腔細菌に関する分子生物学的解析 (2008)
  • Author(s): 野村良太,仲野和彦,根本浩利,大嶋 隆
  • Organizer: 第46回日本小児歯科学会大会
  • Place of Presentation: さいたま
  • Year and Date: 2008-06-12
• [Presentation] 大動脈瘤組織におけるStreptococcus mutans の検出 (2008)
  • Author(s): 根本浩利,仲野和彦,野村良太,大嶋 隆
  • Organizer: 第46 回日本小児歯科学会大会
  • Place of Presentation: さいたま
  • Year and Date: 2008-06-12
• [Presentation] Streptococcus mutans コラーゲン結合タンパク遺伝子の局在に関して (2007)
  • Author(s): 谷口奈穂,野村良太,仲野和彦,大嶋 隆
  • Organizer: 第26 回日本小児歯科学会近畿地方会大会
  • Place of Presentation: 尼崎
  • Year and Date: 2007-10-21
• [Presentation] 感染性心内膜炎患者血液より分離したStreptococcus mutans の由来に関して (2007)
  • Author(s): 根本浩利,仲野和彦,野村良太,大嶋 隆
  • Organizer: 第26 回日本小児歯科学会近畿地方会大会
  • Place of Presentation: 尼崎
  • Year and Date: 2007-10-21
• [Presentation] 菌血症患者血液より分離したStreptococcus mutans の性状の解析 (2007)
  • Author(s): 野村良太,仲野和彦,根本浩利,大嶋 隆
  • Organizer: 第45 回日本小児歯科学会大会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-19
• [Presentation] Streptococcus mutans 臨床分離株における表層タンパク発現状態の解析 (2007)
  • Author(s): 根本浩利,仲野和彦,野村良太,大嶋 隆
  • Organizer: 第45 回日本小児歯科学会大会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-19
• [Presentation] 菌血症患者血液よ力分離したStreptococcus mutansの性状の解析 (2007)
  • Author(s): 野村良太, 仲野和彦, 根本浩利, 大嶋 隆
  • Organizer: 第45回日本小児歯科学会
  • Place of Presentation: 東京
  • Year and Date: 2007-07-19
• [Presentation] Characterization of surface protein antigen in serotype k Streptococcus mutans clinical isolates (2007)
  • Author(s): Nakano K, Nomura R, Nemoto H, Ooshima T
  • Organizer: 54th ORCA congress
  • Place of Presentation: Helsingor, Denmark
  • Year and Date: 2007-07-04
• [Presentation] Serotype distribution of Streptococcus mutans in cardiovascular specimens (2007)
  • Author(s): Nomura R, Nakano K, Nemoto H, Ooshima T
  • Organizer: 85th International Association of Dental Research
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2007-03-21
• [Presentation] 心臓血管病巣からの口腔細菌の検出 (2006)
  • Author(s): 根本浩利,仲野和彦,野村良太,大嶋 隆
  • Organizer: 第25 回日本小児歯科学会近畿地方会大会
  • Place of Presentation: 大阪
  • Year and Date: 2006-11-09
• [Presentation] 感染性心内膜炎患者摘出弁における新規血清型S. mutans の検出 (2006)
  • Author(s): 仲野和彦,野村良太,根本浩利,大嶋 隆
  • Organizer: 第25 回日本小児歯科学会近畿地方会大会
  • Place of Presentation: 大阪
  • Year and Date: 2006-11-09
• [Presentation] Comparison of Streptococcus mutans from dental plaque and heart valve (2006)
  • Author(s): Nakano K, Nomura R, Nemoto H, Ooshima T
  • Organizer: 84th International Association of Dental Research
  • Place of Presentation: Brisbane, Australia
  • Year and Date: 2006-06-30
• [Presentation] 感染性心内膜炎患者プラークおよび感染弁から分離したStreptococcus mutans の分子生物学的性状の検討 (2006)
  • Author(s): 根本浩利,仲野和彦,野村良太,大嶋 隆
  • Organizer: 第44 回日本小児歯科学会
  • Place of Presentation: 松本
  • Year and Date: 2006-05-25