Functional analysis of S. mutans surface proteins in biofilm formation

• Project/Area Number: 19791573
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Osaka University
• Principal Investigator: FUJITA Kazuyo Osaka University, 歯学部附属病院, 医員 (00437386)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: Streptococcus mutans / 表層タンパク / グルカン結合タンパク / 分子生物学 / ミュータンスレンサ球菌 / う蝕

Research Abstract

Streptococcus mutans は、最も重要なう蝕病原性細菌であり、その菌体の表層には様々な重要なタンパクが存在している。菌体表層にはグルカン合成酵素 (Glucosyltransferase: GTF)、高分子タンパク抗原 (Pac)、およびグルカン結合タンパク (Gbp) が存在している。GTF においては、これまでGTFB、 GTFC、およびGTFD の3種が、グルカン結合タンパク (Gbp) は、GbpA、GbpB、GbpC、およびGbpD の4種が報告されている。本研究ではこれらの欠失変異株を作製し、それぞれのう蝕原性における強度を比較検討し、さらにそれぞれのタンパクにおけるバイオフィルム形成における役割について検討を行った。その結果、GTFB が最も強いう蝕原性を示すことが明らかとなった。さらに、バイオフィルムの構築にはすべての表層タンパクがその密度および形などの構造に深く関与していることが示された。

Research Products

• [Journal Article] Effects of recombinase A deficiency on biofilm formation by Streptococcus mutans (2009)
  • Author(s): Inagaki S, Matsumoto-Nakano M, Fujita K, Nagayama K, Funao J, Ooshima T
  • Journal Title: Oral Microbiol Immunol 24 Pages: 104-108
• [Journal Article] Bacterial profiles of oral streptococcal and periodontal bacterial species in saliva specimens from Japanese subjects (2009)
  • Author(s): Miyamoto E, Nakano K, Fujita K, Nomura R, Okawa R, Matsumoto M, Ooshima T
  • Journal Title: Arch Oral Biol 54 Pages: 374-379
• [Journal Article] Distribution of 10 periodontal bacterial species in children and adolescents over a 7-years period (2008)
  • Author(s): Nakano K, Miyamoto E, Tamura K, Nemoto H, Fujita K, Nomura R, Ooshima T
  • Journal Title: Oral Dis 14 Pages: 658-664
• [Journal Article] Distribution of 10 periodontal bacterial spicies in children and adolescents over a 7-year period (2008)
  • Author(s): Nakano K, Miyamoto E, Tamura K, Nemoto H, Fujita K, Nomura R, Ooshima T
  • Journal Title: Oral Disease 14 Pages: 658-664
  • Peer Reviewed
• [Journal Article] Biological function of glucan-binding protein B of Streptococcus mutans (2007)
  • Author(s): Fujita K, Matsumoto-Nakano M, Inagaki S, Ooshima T
  • Journal Title: Oral Microbiol Immunol 22 Pages: 289-292
• [Journal Article] Comparison of glucan-binding proteins in cariogenicity of Streptococcus mutans (2007)
  • Author(s): Matsumoto-Nakano M, Fujita K, Ooshima T
  • Journal Title: Oral Microbiol Immunol 22 Pages: 30-35
• [Journal Article] Biologican functions of glucan-binding protein B of Streptococcus mutans. (2007)
  • Author(s): Fujita K, Matsumoto-Nakano M, Ooshima T.
  • Journal Title: Oral Microbiology Immunology 22 Pages: 289-292
  • Peer Reviewed
• [Journal Article] Comparison of glucan-binding proteins in cariogenicity of Streptococcus matans. (2007)
  • Author(s): Matsumoto-Nakano M., Fujita K., Ooshima T.
  • Journal Title: Oral Microbiology Immunology 22 Pages: 30-35
  • Peer Reviewed
• [Journal Article] Contribution of cell surface protein antigen Pac of Streptococcus mutans
  • Author(s): Matsumoto-Nakano M, Tsuji M, Inagaki S, Fujita K, Nagayama K, Nomura R, Ooshima T
  • Journal Title: Oral Microbiol Immunol (in press)
• [Presentation] Streptococcus mutans のストレス環境下におけるATP 結合タンパクの機能解析 (2008)
  • Author(s): 永山佳世子, 松本道代, 藤田 一世, 大嶋 隆
  • Organizer: 第27 回日本小児歯科学会近畿地方会大会
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-19
• [Presentation] GbpB expression in oral isolates of Streptococcus mutans (2008)
  • Author(s): Fujita K, Matsumoto-Nakano M, Ooshima T
  • Organizer: 86th IADR Conference
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-05
• [Presentation] GbpB expression in oral isolates of Streptococcus mutans (2008)
  • Author(s): Fujita K, Matsumoto-Nakano M, Ooshima T
  • Organizer: 86th International Association of Dental Research
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-02
• [Presentation] Streptococcus mutans のバイオフィルム形成における遺伝子修復機能について (2008)
  • Author(s): 藤田一世, 松本道代, 大嶋 隆
  • Organizer: 第46 回日本小児歯科学会大会
  • Place of Presentation: さいたま
  • Year and Date: 2008-06-12
• [Presentation] Streptococcus mutans の抗生物質耐性におけるATP 結合タンパクの機能解析 (2008)
  • Author(s): 永山佳世子, 松本道代, 藤田 一世, 大嶋 隆
  • Organizer: 第46 回日本小児歯科学会大会
  • Place of Presentation: さいたま
  • Year and Date: 2008-06-12
• [Presentation] Streptococcus mutans の表層タンパク発現のメカニズム (2007)
  • Author(s): 藤田 一世, 松本道代, 稲垣暁子, 大嶋隆
  • Organizer: 第26 回 日本小児歯科学会近畿地方大会
  • Place of Presentation: 尼崎
  • Year and Date: 2007-10-21
• [Presentation] Cariogenic properties of glucan-binding proteins in Streptococcus mutans (2007)
  • Author(s): Fujita K, Matsumoto-Nakano M, Inagaki S, Taniguchi N, Ooshima T
  • Organizer: 21st Congress of International Association of Pediatric Dentistry
  • Place of Presentation: Hong Kong, China
  • Year and Date: 2007-06-14
• [Presentation] Cariogenic properties of glucan-binding proteins in Streptococcus mutans (2007)
  • Author(s): fujita K, Matsumoto-Nskano M, Inagaki K, et. al.
  • Organizer: 21th IAPD Congress
  • Place of Presentation: HongKong, China
  • Year and Date: 2007-06-14