Morphological change including actin filament from osteoblast to osteocyte

• Project/Area Number: 19791579
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: SUGAWARA Yasuyo Okayama University, 大学院・医歯薬学総合研究科, 助教 (70379775)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,670,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,500,000 (Direct Cost: ¥2,500,000)
• Keywords: 歯科矯正学 / 骨細胞 / 骨芽細胞 / アクチン線維 / 骨 / 弾性率

Research Abstract

骨細胞は分化する際に、立方体の骨芽細胞から、ダイナミックな形態変化を引き起こして、突起を持った星状の骨細胞に形態変化を起こす。この形態変化に関して、骨芽細胞から骨細胞へ分化する際には、アクチンのダイナミクスな変化があることが分かり、各分化の段階で弾性も変化することが本研究により分かった。さらに突起が骨芽細胞と接触している詳細および、分化に伴う細胞接着の変化もとらえた。

Research Products

• [Journal Article] The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes (2008)
  • Author(s): Sugawara Y., Ando R., Kamioka H., Ishihara Y., Murshid SA., Hashimoto K., Kataoka N., Tsujioka K., Kajiya F., Yamashiro T., Takano-Yamamoto T.
  • Journal Title: Bone 43(1) Pages: 19-24
  • Peer Reviewed
• [Journal Article] The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes (2008)
  • Author(s): Sugawara Y
  • Journal Title: Bone 43 Pages: 19-24
  • Peer Reviewed
• [Journal Article] Less response of osteocyte than osteoblast to mechanical force : implication of different focal adhesion formation (2007)
  • Author(s): Takano-Yamamoto T., Kamioka H., Sugawara Y.
  • Journal Title: Interface, Oral Health Science Pages: 149-158
  • Peer Reviewed
• [Journal Article] Lim S. Primary culture of chick osteocytes retain functional gap junctions between osteocytes, and between osteocytes and osteoblasts (2007)
  • Author(s): Kamioka H., Ishihara Y., Ris H., Murshid SA., Sugawara Y., Takano-Yamamoto T.
  • Journal Title: Microsc Microanal 13(2) Pages: 108-117
  • Peer Reviewed
• [Journal Article] Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes (2007)
  • Author(s): Murshid SA., Kamioka H., Ishihara Y., Ando R., Sugawara Y.,Takano-Yamamoto T.
  • Journal Title: J Bone Miner Metab 25(3) Pages: 151-158
  • Peer Reviewed
• [Journal Article] Less response of osteocyte than osteoblast to mechanical force: implication of different focal adhelson formation (2007)
  • Author(s): Takano-Yamamoto T, Kamioka H, Sugawara Y
  • Journal Title: Interface Oral Health Science 1 Pages: 149-158
  • Peer Reviewed
• [Journal Article] Primary culture of chick osteocytes retain functional gapjunctionsbetween osteocytes, and between osteocytes and osteoblasts (2007)
  • Author(s): Kamioka H, Ishihara Y, Ris H, Murshid SA, Sugawara Y
  • Journal Title: Microscopy and Microanalysis 13 Pages: 108-117
  • Peer Reviewed
• [Journal Article] The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes
  • Author(s): Sugawara Y
  • Journal Title: Bone (印刷中)
  • Peer Reviewed
• [Presentation] The three-dimensional morphometry ofosteocytes network in chick and mouse calvaria (2008)
  • Author(s): Ando R., Sugawara Y.
  • Organizer: The 1st international symposium of medical and dental education
  • Place of Presentation: Okayama
  • Year and Date: 2008-02-01
• [Presentation] The three-dimensional morphometry of osteocytes network in chick and mouse calvaria (2008)
  • Author(s): Ando R, Sugawara Y
  • Organizer: The 1st international symposium of medical and dental education
  • Place of Presentation: Okayama
  • Year and Date: 2008-02-01
• [Presentation] 胎生期と6週齢マウス長管骨中の3次元骨細胞ネットワークの比較 (2008)
  • Author(s): 菅原康代
  • Organizer: 第67回日本矯正歯科学会
  • Place of Presentation: 幕張
• [Presentation] ニワトリとマウスの骨細胞ネットワークの三次元的形態計測 (2008)
  • Author(s): 安藤涼子、菅原康代
  • Organizer: 第67回日本矯正歯科学会
  • Place of Presentation: 幕張
• [Presentation] 細胞内カルシウムが生骨組織中における細胞間コミュニケーションの調節へ与える影響 (2008)
  • Author(s): 石原嘉人、上岡寛、本城正、菅原康代
  • Organizer: 第67回日本矯正歯科学会
  • Place of Presentation: 幕張
• [Presentation] 胎生期と6週齢のマウス長管骨中の3次元骨細胞ネットワークの比較 (2008)
  • Author(s): 菅原康代
  • Organizer: 日本矯正歯科学会
  • Place of Presentation: 幕張
• [Presentation] ニワトリとマウスの骨細胞ネットワークの三次元的形態計測 (2008)
  • Author(s): 安藤涼子、菅原康代
  • Organizer: 日本矯正歯科学会
  • Place of Presentation: 幕張
• [Presentation] 細胞内カルシウムが生骨組織中における細胞間コミュニケーションの調節へ与える影響 (2008)
  • Author(s): 石原嘉人、上岡寛、本城正、菅原康代
  • Organizer: 日本矯正歯科学会
  • Place of Presentation: 幕張
• [Presentation] ニワトリ胚頭蓋骨中の骨細胞の三次元的形態計測 (2007)
  • Author(s): 菅原康代
  • Organizer: 岡山歯学会
  • Place of Presentation: 岡山
  • Year and Date: 2007-08-18
• [Presentation] 骨芽細胞から骨細胞への弾性の変化について (2007)
  • Author(s): 菅原康代
  • Organizer: 日本矯正歯科学会
  • Place of Presentation: 大阪
• [Presentation] The elastic modulus of osteoblasts and osteocytes (2007)
  • Author(s): Sugawara Y.
  • Organizer: The American Society for Bone and Mineral Research
  • Place of Presentation: Honollu
• [Presentation] 骨細胞における機械的刺激への応答能-focal adhesionと細胞弾性率の関与 (2007)
  • Author(s): 山本照子、上岡寛、菅原康代
  • Organizer: 日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] 骨芽細胞から骨細胞への弾性の変化について (2007)
  • Author(s): 菅原康代
  • Organizer: 日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] 骨芽細胞から骨細胞への弾性の変化について (2007)
  • Author(s): 菅原 康代
  • Organizer: 日木骨代謝学会
  • Place of Presentation: 大阪