Electrophysiological analysis of higher nervous function induced by orthodontics apparatus

• Project/Area Number: 19791589
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Nagasaki University
• Principal Investigator: ZEREDO Jorge Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (10363459)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,640,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: ストレス / 痛み / 矯正治療 / 行動 / ラット

Research Abstract

ウィスター系ラットを用いて、痛みとストレスの行動学的な解析行った。全身麻酔下で、脳手術にて脳慢性電極を埋め込み、視床下部ニューロン活動の記録した。同時に、咬筋の筋電図を記録した。一群は口腔内にCo-Cr合金からなる矯正装置を切歯部に接着した。この装置は50グラム相当の力を発揮するものであった。もう一群は対照としてシャム装置を接着した。結果として、実験群のラットには装置接着後の一日目に痛みやストレスを示す行動の増大を認めた。拘束に対するストレス応答が増大し、辺縁系を含めた高次機能へのストレスの影響がうかがえる。ラットの臼歯部に矯正力を加えたことによって、摂食行動は低下した。また、咬筋の筋電図活動は高かった。視床下部ニューロンの発火活動は実験群が対照群より低い頻度を認めた。

Research Products

• [Journal Article] Disinhibitory involvement of the anterior cingulate cortex in the descending antinociceptive effect induced by electroacupuncture stimulation in rats (2008)
  • Author(s): Hirano T, Zeredo JL, Kimoto M, Moritaka K, Nasution FH, Toda K
  • Journal Title: Am J Chin Med 36(3) Pages: 569-577
  • Peer Reviewed
• [Journal Article] Emotional stress-and pain-related behaviors evoked by experimental tooth movement (2008)
  • Author(s): Yozgatian JH, Zeredo JL, Hotokezaka H, Koga Y, Toda K, Yoshida N
  • Journal Title: Angle Orthod 78(3) Pages: 487-94
  • Peer Reviewed
• [Journal Article] A calcium-receptor agonist induces gustatory neural responses in bullfrogs (2007)
  • Author(s): Okada Y, Imendra KG, Miyazaki T, Hotokezaka H, Fujiyama R, Zeredo JL, Toda K
  • Journal Title: Cell Mol Neurobiol 27(6) Pages: 771-781
  • Peer Reviewed
• [Journal Article] Functional changes of the temporomandibular jointmechanoreceptors induced by a lateral mandibular shift in rats (2007)
  • Author(s): Kokai S, Yabushita T, Zeredo JL, Toda K, Soma K
  • Journal Title: Angle Orthod 77(3) Pages: 436-441
  • Peer Reviewed
• [Journal Article] Splenius muscle activities induced by temporomandibular joint stimulation in rats (2007)
  • Author(s): Kobayashi M, Yabushita T, Zeredo JL, Toda K, Soma K
  • Journal Title: Brain Res Bull 72(1) Pages: 44-48
  • Peer Reviewed
• [Journal Article] High-intensity laser for acupuncture-like stimulation (2007)
  • Author(s): Zeredo JL, Sasaki KM, Toda K
  • Journal Title: Lasers Med Sci 22(1) Pages: 37-41
  • Peer Reviewed
• [Journal Article] Effect of traditional "Juci" (contralateral acupuncture) on orofacial nociceptive behavior in the rat (2007)
  • Author(s): Miura K, Ohara T, Zeredo JL, Okada Y, Toda K, Sumikawa K
  • Journal Title: J Anesth 21(1) Pages: 31-36
  • Peer Reviewed
• [Journal Article] Measuring biting behavior induced by acute stress in the rat
  • Author(s): Zeredo JL, Kumei Y, Shibazaki T, Yoshida N, Toda K
  • Journal Title: Behavior Research Methods (in press)
  • Peer Reviewed
• [Presentation] Biting behavior induced by acute stress in the rat during experimental tooth movement (2008)
  • Author(s): ゼレドジョージ
  • Organizer: 行動学測定学会2008
  • Place of Presentation: マーストリヒト
  • Year and Date: 2008-08-27
• [Presentation] レーザー切開による術後痛み感覚とアポトーシスとの関連 (2008)
  • Author(s): ゼレド ジョージ
  • Organizer: アポトーシス2008
  • Place of Presentation: ルクセンブルグ
  • Year and Date: 2008-01-25
• [Presentation] Rat hypothalamic response to the Lunar and Martian gravity during parabolic flights (2008)
  • Author(s): Kumei Y, Zeredo JL, Seki S, Kageyama D, Matsuura M, Fusejima Y, Ikeda T, Toda K
  • Organizer: Life in Space for Life on Earth 2008 Symposium
  • Place of Presentation: Angers, Francee
• [Presentation] Stressful Behaviors During Moderate-Low Gravity Exposure in Rats (2008)
  • Author(s): Zeredo JL, Kumei Y, Toda K
  • Organizer: Life in Space for Life on Earth 2008 Symposium
  • Place of Presentation: Angers, France
• [Presentation] Chemical Sensation can be Modulated by High-Gravity in Rats (2008)
  • Author(s): Kimoto, M. ; Takase, S, Toda, K, Zeredo JL, Kumei, Y
  • Organizer: Life in Space for Life on Earth 2008 Symposium
  • Place of Presentation: Angers, France
• [Presentation] Japanese Research Group for Life Sciences in the Lunar and Martian Gravity (2008)
  • Author(s): Kumei Y, Akiyama H, Ikeda T, Ishioka N, Ito M, Iwasaki K, Igarashi M, Kageyama D, Kizaki M, Matsuura M, Morita S, Mukai C, Nomura Y, Toda K, Wakata K, Yamashita M, Zeredo JL
  • Organizer: Life in Space for Life on Earth 2008 Symposium
  • Place of Presentation: Angers, France
• [Presentation] Measuring the neuronal activity of hypothalamus nd behavior of rats on the diffuse low gravity conditions (2008)
  • Author(s): Kumei Y, Zeredo JL, Seki S, Matsuura M, Kimoto M, Kageyama D, Fusejima Y, Ikeda T, Toda K
  • Organizer: Measuring Behavior 2008 Symposium
  • Place of Presentation: Maastricht, The Netherlands
• [Presentation] Biting behavior induced by acute stress in the rat during experimental tooth movement (2008)
  • Author(s): Zeredo JL, Kumei Y, Shibazaki T, Yoshida N, Kimoto M, Toda K
  • Organizer: Measuring Behavior 2008 Symposium
  • Place of Presentation: Maastricht, The Netherlands
• [Presentation] (2008)
  • Author(s): Kimoto M, Kumei Y, Zeredo JL, Toda K
  • Organizer: Changes in feeding behavior after high gravity loading in orchidectomized and ovariectomized rats 2008 Symposium
  • Place of Presentation: Maastricht, The Netherlands
• [Presentation] ラット針鎮痛発現における前帯状回の役割 (2007)
  • Author(s): ゼレド ジョージ
  • Organizer: 日本歯科東洋医学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-10-25