Amperometric Algal Array Biosensor for Environmental Water ToxicityTest

• Project/Area Number: 19850026
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Environmental chemistry
• Research Institution: Tokyo University of Science
• Principal Investigator: SHITANDA Isao Tokyo University of Science, 理工学部, 助教 (70434024)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,105,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,350,000 (Direct Cost: ¥1,350,000)
• Keywords: 細胞バイオセンサー / 環境毒性 / スクリーン印刷 / 環境分析 / バイオセンサ / 細胞 / フローインジェクション / 鞭毛藻類 / バイオセンサー / 藻類細胞

Research Abstract

藻類細胞の光合成活性を電気化学的にモニタリング可能なバイオセンシングデバイスの開発を行った.スクリーン印刷を用いて藻類細胞を印刷によって安定に電極上に固定化する技術を確立した.固定化した藻類細胞の光合成による酸素発生を電気化学的にモニタリングすることで, 除草剤の毒性を4minで評価可能となった.また, 藻類の鞭毛運動および走光性活性変化を迅速に検知可能な新規細胞バイオセンサを開発した

Research Products

• [Journal Article] Electrochemical system for simultaneous monitoring of immobilized algal flagellar movement and phototaxis (2008)
  • Author(s): Isao Shitanda, Koji Ishizaki, Masayuki Itagaki, Kunihiro Watanabe
  • Journal Title: Electrochemistry 76(8) Pages: 566-568
  • NAID: 10021027885
  • Peer Reviewed
• [Journal Article] Screen-printed Algal Biosensor with Flow Injection Analysis System for Toxicity Test (2008)
  • Author(s): Isao Shitanda, Satoshi Takamatsu, Masayuki Itagaki, Kunihiro Watanabe
  • Journal Title: ECS Transactions 16(11) Pages: 21-25
• [Journal Article] Toxicity Test of Amperometric Screen-printed Algal Biosensor with Flow Injection Analysis (2008)
  • Author(s): Satoshi Takamatsu, Isao Shitanda, Masayuki Itagaki, Kunihiro Watanabe
  • Journal Title: Chemical Sensors 24 Pages: 88-90
  • NAID: 40015986453
• [Journal Article] Screen-printed Algal Biosensor with Flow Injection Analysis System for TOxicity Test (2008)
  • Author(s): Isao Shitanda, Satoshi Takamatsu, Masayuki Itagaki, Kunihiro Watanabe
  • Journal Title: ECS Transactions 16(11) Pages: 21-25
  • Peer Reviewed
• [Journal Article] Toxicity Test of Amperometric Screen-printing Algal Biosensor with Flow Injection Analysis (2008)
  • Author(s): Satoshi Takamatsu, Isao Shitanda, Masayuki Itagaki, Kunihiro Watanabe
  • Journal Title: Chemical Sensors 24 Pages: 88-90
  • NAID: 40015986453
• [Journal Article] Amperometric Screen-printed Algal Biosensor with Flow Injection Analysis System for Detection of Environmental Toxic Compounds
  • Author(s): Isao Shitanda, Satoshi Takamatsu, Kunihiro Watanabe, Masayuki Itagaki
  • Journal Title: Electrochimica Acta (in press)
• [Journal Article] Amperometric Screen-printed Algal Biosensor with Flow Injection Analysis System for Detection of Environmental Toxic Compounds
  • Author(s): Isao Shitanda, Satoshi Takamatsu, Kunihiro Watanabe, Masayuki Itasaki
  • Journal Title: Electrochimica Acta (印刷中)
  • Peer Reviewed
• [Presentation] Screen-printed Algal Biosensor with Flow Injection Analysis System for Water Toxicity Test (2008)
  • Author(s): Isao Shianda, Satoshi Takamatsu, Masayuki Itagaki, Kunihiro Watanabe
  • Organizer: Pacific Rim Meeting On Electrochemical And Solid-State Science (PRiME) 2008
  • Place of Presentation: Honolulu, Hilton Hawaiian Village(口頭発表)
  • Year and Date: 2008-10-14
• [Presentation] Screen-printed Algal Biosensor with Flow Injection Analysis System for Toxicity Test (2008)
  • Author(s): Isao Shitanda, Satoshi Takamatsu, Masayuki Itagaki, Kunihiro Watanabe
  • Organizer: Pacific Rim Meeting On Electrochemical And Solid-State Science (PRiME 2008)
  • Place of Presentation: Honolulu, Hilton Hawaiian Village
  • Year and Date: 2008-10-14
• [Presentation] Simultaneous Monitoring System of Immobilized Algal Flagellar Movement and Phototaxis (2008)
  • Author(s): Koji Ishizaki, Isao Shitanda, Masayuki Itagaki, Kunihiro Watanabe
  • Organizer: Pacific Rim Meeting On Electrochemical And Solid-State Science (PRiME) 2008
  • Place of Presentation: Honolulu, Hilton Hawaiian Village(ポスター)
  • Year and Date: 2008-10-13
• [Presentation] 光合成活性測定型藻類細胞バイオセンサによる除草剤の毒性評価 (2008)
  • Author(s): 四反田功, 高松聡之, 板垣昌幸, 渡辺邦洋
  • Organizer: 日本分析化学会第57年会
  • Place of Presentation: 福岡大学
  • Year and Date: 2008-09-10
• [Presentation] 光合成活性測定型藻類細胞バイオセンサによる除草剤の毒性評価 (2008)
  • Author(s): 四反田功, 高松聡之, 板垣昌幸, 渡辺邦洋
  • Organizer: 日本分析化学会第57年会
  • Place of Presentation: 福岡大学七隈キャンパス
  • Year and Date: 2008-09-10
• [Presentation] Fabrication of Algal Biosensor by Screen Printing (2008)
  • Author(s): Satoshi Takamatsu, Isao Shitanda, Masayuki Itagaki, Kunihiro Watanabe
  • Organizer: 213th ECS Meeting
  • Place of Presentation: Phoenix, Hyatt Regency Phoenix
  • Year and Date: 2008-05-19
• [Presentation] ボルボックスの走光性による生物対流変化の電気化学的検出 (2008)
  • Author(s): 石崎浩司, 四反田功, 板垣昌幸, 渡辺邦洋
  • Organizer: 電気化学会第75回大会
  • Place of Presentation: 山梨大学(口頭発表)
  • Year and Date: 2008-03-31
• [Presentation] ボルボックスの走光性による生物対流変化の電気化学的評価 (2008)
  • Author(s): 石崎 浩司, 四反田 功, 板垣 昌幸, 渡邉 邦洋
  • Organizer: 電気化学会第75回大会
  • Place of Presentation: 山梨大学
  • Year and Date: 2008-03-31
• [Presentation] 藻類細胞バイオセンサーのスクリーン印刷による作製とFIAによる評価 (2008)
  • Author(s): 高松聡之, 四反田功, 板垣昌幸, 渡辺邦洋
  • Organizer: 電気化学会第75回大会
  • Place of Presentation: 山梨大学
  • Year and Date: 2008-03-29
• [Presentation] スクリーン印刷法による藻類細胞バイオセンサーの作製と評価 (2007)
  • Author(s): 高松聡之, 四反田功, 板垣昌幸, 渡辺邦洋
  • Organizer: 日本分析化学会第56年会
  • Place of Presentation: 徳島大学
  • Year and Date: 2007-09-21