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Analysis of superficial and deep layer in Xenopus embryo

Research Project

Project/Area Number 19870007
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionThe University of Tokyo

Principal Investigator

TAKAHASHI Shuji  The University of Tokyo, 大学院・総合文化研究科, 特任准教授 (90447318)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,115,000 (Direct Cost: ¥2,710,000、Indirect Cost: ¥405,000)
Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000)
Fiscal Year 2007: ¥1,360,000 (Direct Cost: ¥1,360,000)
KeywordsXnr2 / 表層 / 内層 / アフリカツメガエル / Xenopus / Venopus / 発生生物学 / 内胚葉
Research Abstract

DNAアレイを用いて初期胞胚において遺伝子発現の表層と内層の発現レベルの異なる遺伝子を検出した。この結果、380個の遺伝子が表層に多く発現していることが示唆された。これらの遺伝子のうち、外層と内層間で差が大きかった100個の遺伝子をホールマウントinsituハイブリダイゼーション法を用いて解析した。さらに初期胞胚の内胚葉の表層と内層において発現レベルの異なる100個の遺伝子を特定した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (17 results)

All 2009 2008 2007

All Journal Article (10 results) (of which Peer Reviewed: 4 results) Presentation (4 results) Book (3 results)

  • [Journal Article] XRASGRP2 is essential for blood vessel formation during Xenopus development2009

    • Author(s)
      Suzuki, K., Takahashi, S., Haramoto, Y., Onuma, Y., Nagamine, K., Okabayashi, K., Hashizume, K., Iwanaka, T. and Asashima, M.
    • Journal Title

      Int J Dev Biol (in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Bestrophin/VMD2 genes are expressed in Xenopus development2009

    • Author(s)
      Onuma, Y., Haramoto, Y., Nejigane, S., Takahashi, S. and Asashima, M.
    • Journal Title

      Biochem Biophys Res Commun (in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Isolation and differentiation of Xenopus animal cap cells2009

    • Author(s)
      Ariizumi, T., Takahashi, S., Chan,T. C., Ito, Y., Michiue, T. and Asashima, M.
    • Journal Title

      Curr Protoc Stem Cell Biol Chapter 1, Unit 1D 5

    • Related Report
      2008 Final Research Report
  • [Journal Article] Bestrophin/VMD2 genes are expressed in Xenopus development.2009

    • Author(s)
      Onuma Y, Haramoto Y, Nejigane S, Takahashi S, Asashima M
    • Journal Title

      Biochemical and Biophysical Research Communications. 印刷中(掲載確定)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] XRASGRP2 is essential for blood vessel formation during Xenopus development2009

    • Author(s)
      Suzuki K, Takahashi S, Haramoto Y, Onuma Y, Nagamine K, Okabayashi K, Hashizume K, Iwanaka T, Asashima M
    • Journal Title

      Int J Dev Biol 印刷中(掲載確定)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zygotic VegT is required for Xenopus paraxial mesoderm formation and regulated by Nodal signaling and Eomesodermin2008

    • Author(s)
      Fukuda, M., Takahashi, S., Haramoto, Y., Onuma, Y., Kim, Y.-J., Yeo, C.-Y., Ishiura, S. and Asashima, M.
    • Journal Title

      Int J Dev Biol (in press)

    • Related Report
      2008 Final Research Report
  • [Journal Article] Zygotic vegT is required for Xenopus paraxial mesoderm formation and regulated by Nodal signaling and Eomesodermin2008

    • Author(s)
      Fukuda M, Takahashi S, Haramoto Y, Onuma Y, Kim Y-J, Yeo C-Y, Ishiura S, Asashima M.
    • Journal Title

      Int J Dev Biol 印刷中(掲載確定)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Xenopus Lefty requires proprotein cleavage but not N-linked glycosylation to inhibit nodal signaling2007

    • Author(s)
      Westmoreland, J. J., Takahashi, S. and Wright, C. V.
    • Journal Title

      Dev Dyn 236

      Pages: 2050-61

    • Related Report
      2008 Final Research Report
  • [Journal Article] Molecular evidence that the lungs and the swimbladder are homologous organs2007

    • Author(s)
      Shu, K., Murata, Y., Takahashi, S.,Asashima, M., Joss, J. M. P., Tanaka, M. and Okabe, M.
    • Journal Title

      Journal of Morphology 268

      Pages: 1134-1134

    • Related Report
      2008 Final Research Report
  • [Journal Article] Xenopus Lefty requires proprotein cleavage but not N-linked glycosylation to inhibit nodal signaling.2007

    • Author(s)
      Westmoreland JJ, Takahashi S, Wright C
    • Journal Title

      Developmental Dynamics 236

      Pages: 2050-2061

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Eomesodermin and Nodal signaling coregulate Xenopus zygotic VegT expression in mesoderm formation2008

    • Author(s)
      Fukuda M., Takahashi S., Haramoto Y., Onuma Y., Yeo C-Y., Ishiura S., Asashima M.
    • Organizer
      12th international Xenopus conference
    • Place of Presentation
      Leiwen, Germany
    • Related Report
      2008 Final Research Report
  • [Presentation] Eomesodermin and Nodal signaling coregulate Xenopus zygotic VegT expression in mesoderm formation.2008

    • Author(s)
      Fukuda M., Takahashi S., Haramoto Y., Onuma Y., Yeo C-Y., Ishiura S., Asashima M.
    • Organizer
      12th international Xenopus conference
    • Place of Presentation
      Leiwen, Germany
    • Related Report
      2008 Annual Research Report
  • [Presentation] Xenopus初期胚における表層と内層の差異についての解析2007

    • Author(s)
      高橋 秀治
    • Organizer
      第一回日本ツメガエル研究集会
    • Place of Presentation
      札幌、定山渓ビューホテル
    • Related Report
      2008 Final Research Report
  • [Presentation] Xenopus初期胚における表層と内層の差異についての解析2007

    • Author(s)
      高橋 秀治
    • Organizer
      第一回ツメガエル研究集会
    • Place of Presentation
      札幌
    • Related Report
      2007 Annual Research Report
  • [Book] Curr Protoc Stem Cell Biol. Chapter 1 unit 1D.5. Isolation and Differentiation of Xenopus Animal Cap Cells2009

    • Author(s)
      Ariizumi T, Takahashi S, Chan TC, Ito Y, Michiue T, Asashima M ,
    • Publisher
      John Wiley & Sons
    • Related Report
      2008 Annual Research Report
  • [Book] DVD「生命科学」2008

    • Author(s)
      高橋秀治, 他
    • Publisher
      東京大学大学院総合文化研究科生命科学構造化センター
    • Related Report
      2008 Final Research Report
  • [Book] 写真で見る生命科学2008

    • Author(s)
      高橋秀治, 他
    • Publisher
      東京大学大学院総合文化研究科生命科学構造化センター
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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