Analysis of superficial and deep layer in Xenopus embryo

• Project/Area Number: 19870007
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: TAKAHASHI Shuji The University of Tokyo, 大学院・総合文化研究科, 特任准教授 (90447318)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,115,000 (Direct Cost: ¥2,710,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,360,000 (Direct Cost: ¥1,360,000)
• Keywords: Xnr2 / 表層 / 内層 / アフリカツメガエル / Xenopus / Venopus / 発生生物学 / 内胚葉

Research Abstract

DNAアレイを用いて初期胞胚において遺伝子発現の表層と内層の発現レベルの異なる遺伝子を検出した。この結果、380個の遺伝子が表層に多く発現していることが示唆された。これらの遺伝子のうち、外層と内層間で差が大きかった100個の遺伝子をホールマウントinsituハイブリダイゼーション法を用いて解析した。さらに初期胞胚の内胚葉の表層と内層において発現レベルの異なる100個の遺伝子を特定した。

Research Products

• [Journal Article] XRASGRP2 is essential for blood vessel formation during Xenopus development (2009)
  • Author(s): Suzuki, K., Takahashi, S., Haramoto, Y., Onuma, Y., Nagamine, K., Okabayashi, K., Hashizume, K., Iwanaka, T. and Asashima, M.
  • Journal Title: Int J Dev Biol (in press)
• [Journal Article] Bestrophin/VMD2 genes are expressed in Xenopus development (2009)
  • Author(s): Onuma, Y., Haramoto, Y., Nejigane, S., Takahashi, S. and Asashima, M.
  • Journal Title: Biochem Biophys Res Commun (in press)
• [Journal Article] Isolation and differentiation of Xenopus animal cap cells (2009)
  • Author(s): Ariizumi, T., Takahashi, S., Chan,T. C., Ito, Y., Michiue, T. and Asashima, M.
  • Journal Title: Curr Protoc Stem Cell Biol Chapter 1, Unit 1D 5
• [Journal Article] Bestrophin/VMD2 genes are expressed in Xenopus development. (2009)
  • Author(s): Onuma Y, Haramoto Y, Nejigane S, Takahashi S, Asashima M
  • Journal Title: Biochemical and Biophysical Research Communications. 印刷中(掲載確定)
  • Peer Reviewed
• [Journal Article] XRASGRP2 is essential for blood vessel formation during Xenopus development (2009)
  • Author(s): Suzuki K, Takahashi S, Haramoto Y, Onuma Y, Nagamine K, Okabayashi K, Hashizume K, Iwanaka T, Asashima M
  • Journal Title: Int J Dev Biol 印刷中(掲載確定)
  • Peer Reviewed
• [Journal Article] Zygotic VegT is required for Xenopus paraxial mesoderm formation and regulated by Nodal signaling and Eomesodermin (2008)
  • Author(s): Fukuda, M., Takahashi, S., Haramoto, Y., Onuma, Y., Kim, Y.-J., Yeo, C.-Y., Ishiura, S. and Asashima, M.
  • Journal Title: Int J Dev Biol (in press)
• [Journal Article] Zygotic vegT is required for Xenopus paraxial mesoderm formation and regulated by Nodal signaling and Eomesodermin (2008)
  • Author(s): Fukuda M, Takahashi S, Haramoto Y, Onuma Y, Kim Y-J, Yeo C-Y, Ishiura S, Asashima M.
  • Journal Title: Int J Dev Biol 印刷中(掲載確定)
  • Peer Reviewed
• [Journal Article] Xenopus Lefty requires proprotein cleavage but not N-linked glycosylation to inhibit nodal signaling (2007)
  • Author(s): Westmoreland, J. J., Takahashi, S. and Wright, C. V.
  • Journal Title: Dev Dyn 236 Pages: 2050-61
• [Journal Article] Molecular evidence that the lungs and the swimbladder are homologous organs (2007)
  • Author(s): Shu, K., Murata, Y., Takahashi, S.,Asashima, M., Joss, J. M. P., Tanaka, M. and Okabe, M.
  • Journal Title: Journal of Morphology 268 Pages: 1134-1134
• [Journal Article] Xenopus Lefty requires proprotein cleavage but not N-linked glycosylation to inhibit nodal signaling. (2007)
  • Author(s): Westmoreland JJ, Takahashi S, Wright C
  • Journal Title: Developmental Dynamics 236 Pages: 2050-2061
  • Peer Reviewed
• [Presentation] Eomesodermin and Nodal signaling coregulate Xenopus zygotic VegT expression in mesoderm formation (2008)
  • Author(s): Fukuda M., Takahashi S., Haramoto Y., Onuma Y., Yeo C-Y., Ishiura S., Asashima M.
  • Organizer: 12th international Xenopus conference
  • Place of Presentation: Leiwen, Germany
• [Presentation] Eomesodermin and Nodal signaling coregulate Xenopus zygotic VegT expression in mesoderm formation. (2008)
  • Author(s): Fukuda M., Takahashi S., Haramoto Y., Onuma Y., Yeo C-Y., Ishiura S., Asashima M.
  • Organizer: 12th international Xenopus conference
  • Place of Presentation: Leiwen, Germany
• [Presentation] Xenopus初期胚における表層と内層の差異についての解析 (2007)
  • Author(s): 高橋 秀治
  • Organizer: 第一回日本ツメガエル研究集会
  • Place of Presentation: 札幌、定山渓ビューホテル
• [Presentation] Xenopus初期胚における表層と内層の差異についての解析 (2007)
  • Author(s): 高橋 秀治
  • Organizer: 第一回ツメガエル研究集会
  • Place of Presentation: 札幌
• [Book] Curr Protoc Stem Cell Biol. Chapter 1 unit 1D.5. Isolation and Differentiation of Xenopus Animal Cap Cells (2009)
  • Author(s): Ariizumi T, Takahashi S, Chan TC, Ito Y, Michiue T, Asashima M ,
  • Publisher: John Wiley & Sons
• [Book] DVD「生命科学」 (2008)
  • Author(s): 高橋秀治, 他
  • Publisher: 東京大学大学院総合文化研究科生命科学構造化センター
• [Book] 写真で見る生命科学 (2008)
  • Author(s): 高橋秀治, 他
  • Publisher: 東京大学大学院総合文化研究科生命科学構造化センター