A construction of real-time detection probe for intracellular DNA checkpoint activation

• Project/Area Number: 19870029
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: National Institute of Genetics
• Principal Investigator: FURUYA Kanji National Institute of Genetics, 系統生物研究センター, 助教授 (90455204)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,125,000 (Direct Cost: ¥2,720,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,370,000 (Direct Cost: ¥1,370,000)
• Keywords: バイオイメージング / 細胞内情報伝達 / 細胞周期 / 環境応答

Research Abstract

細胞は紫外線などで遺伝情報であるDNA が傷つくと細胞分裂を停止して、それを修復する。この機能をDNA チェックポイント機能と呼び、それがおかしくなると癌を多発する。チェックポイント機能が働いている様子をリアルタイムで検出し得るツールを作製した。これをさらに改良する事で生きたままチェックポイント機能の活性化の様子を追うことができる様になると考えている。

Research Products

• [Journal Article] Isolation of heterothallic haploid and auxotrophic mutants of Schizosaccharomyces japonicus (2009)
  • Author(s): Furuya K, Niki H
  • Journal Title: Yeast 26 Pages: 221-233
  • Peer Reviewed
• [Journal Article] Isolation of heterothallic haploid and auxotrophic mutants of Schizosacc haromyces japonicus (2009)
  • Author(s): Furuya K.
  • Journal Title: Yeast 26 Pages: 221-233
  • Peer Reviewed
• [Journal Article] Rad3-dependent phosphorylation of the checkpoint clamp regulates repair-pathway choice (2007)
  • Author(s): Mihoko Kai, Kanji Furuya, Francesca Paderi, Antony M. Carr & Teresa S.F. Wang
  • Journal Title: Nature Cell Biology 9 Pages: 691-697
  • Peer Reviewed
• [Presentation] Multistep activation of genomic stability pathway (2008)
  • Author(s): 古谷寛治 仁木宏典
  • Organizer: 分子生物学会年会
  • Place of Presentation: 神戸ポートピアアイランド
  • Year and Date: 2008-12-12
• [Presentation] DNAチェックポイント機構のDNA複製時のゲノム安定維持に置ける役割 (2008)
  • Author(s): 古谷寛治
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] Multistep activation of genomic stability pathway (2008)
  • Author(s): Kanji Furuya, Hironori, Niki
  • Organizer: International Chromosome Dynamics Symposium in Ise
  • Place of Presentation: アクアヴィラ伊勢志摩
  • Year and Date: 2008-05-28
• [Presentation] Multistep activation of genomic stability pathway (2008)
  • Author(s): Kanji Furuya, Hironori, Niki
  • Organizer: 遺伝研国際シンポジウム
  • Place of Presentation: 国立遺伝学研究所
  • Year and Date: 2008-05-26
• [Presentation] Multistep activation of genomic stability pathway (2008)
  • Author(s): Kanji Furuya, Hironori, Niki
  • Organizer: 蛋白研セミナー
  • Place of Presentation: 大阪大学蛋白質研究所
  • Year and Date: 2008-03-14
• [Presentation] Multistep activation of genomic stability pathway (2008)
  • Author(s): 古谷寛治 仁木宏典
  • Organizer: 染色体ワークショップ
  • Place of Presentation: ウエルシティ湯河原
  • Year and Date: 2008-01-30
• [Presentation] Multistep activation of genomic stability pathway (2008)
  • Author(s): Kanji Furuya, Hironori Niki
  • Organizer: The 6th 3R symposium
  • Place of Presentation: 嬬恋リゾート
• [Presentation] Chromosome Dynamics in alternative Fission Yeast system (2008)
  • Author(s): 古谷寛治,仁木宏典
  • Organizer: 細胞生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Multistep activation of genomic stability pathway (2007)
  • Author(s): 古谷寛治 仁木宏典
  • Organizer: 分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] MULTI STEP ACTIVATION OF GENOMIC STABILITY PATHWAY BY DNA CHECKPOINT MECHANISM (2007)
  • Author(s): 古谷寛治
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] Multistep activation of genomic stability pathway (2007)
  • Author(s): Kanji Furuya, Hironori, Niki
  • Organizer: Asia-Pacific Regional Fission Yeast Meeting
  • Place of Presentation: Singapore