Characterization of supercooling-promoting flavonoids in xylemparenchyma cells of boreal trees

• Project/Area Number: 19880002
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: 林産科学・木質工学
• Research Institution: Hokkaido University
• Principal Investigator: KASUGA Jun Hokkaido University, 大学院・農学研究院, 博士研究員 (40451421)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 樹木 / 凍結適応 / 木部柔細胞 / フラボノイド / 過冷却促進 / 深過冷却 / 氷核形成阻害

Research Abstract

カツラという樹木の木部組織には水の過冷却を促進するフラボノイドが存在する。本研究によって、このフラボノイドの木部組織における局在性と量的な季節変化が明らかになった。また、フラボノイドの過冷却促進効果はアグリコンと糖鎖の組み合わせにより大きく変化することを明らかにした。さらに、カツラ由来のcDNA ライブラリの調製など、フラボノイドの蓄積量を改変した形質転換樹木の作出の準備を進めた。

Research Products

• [Journal Article] Improved cryopreservation by diluted vitrification solution with supercooling-facilitating flavonol glycoside (2008)
  • Author(s): DAISUKE KAMI、JUN KASUGA、KEITA ARAKAWA、SEIZO FUJIKAWA
  • Journal Title: Cryobiology 57 Pages: 242-245
  • Peer Reviewed
• [Journal Article] Deep supercoolingxylem parenchyma cells of katsura tree (Cercidiphyllum japonicum) contain flavonol glycosides exhibiting highanti-ice nucleation activity (2008)
  • Author(s): JUN KASUGA、YASUYUKI HASHIDOKO、ATSUSHI NISHIOKA、MEGUMI YOSHIBA、KEITA ARAKAWA、SEIZO FUJIKAWA
  • Journal Title: Plant Cell and Environment 31 Pages: 1335-1348
  • Peer Reviewed
• [Journal Article] 過冷却促進物質 (2008)
  • Author(s): 藤川清三、春日純
  • Journal Title: 検査と技術 36 Pages: 546-549
• [Journal Article] Deep supercooling xylem parenchyma cells of katsura tree (Cercidiphyllum japonicum) contain flavonol glycosides exhibiting high anti-ice nucleation activity (2008)
  • Author(s): Kasuga, Jun
  • Journal Title: Plant, Cell & Environment 31 Pages: 1335-1348
  • Peer Reviewed
• [Journal Article] Improved cyopreservation by diluted vitrification solution with supercooling-facilitating flavonol glycoside (2008)
  • Author(s): Kami, Daisuke
  • Journal Title: Cryobiology 57 Pages: 242-245
  • Peer Reviewed
• [Journal Article] 過冷却促進物質 (2008)
  • Author(s): 藤川清三
  • Journal Title: 検査と技術 36 Pages: 546-549
• [Journal Article] Anti-ice nucleationactivity in xylem extracts from treesthat contain deep supercooling xylem (2007)
  • Author(s): JUN KASUGA、KAORU MIZUNO、KEITA ARAKAWA、SEIZO FUJIKAWA
  • Journal Title: parenchyma cells、 Cryobiology 55 Pages: 305-314
  • Peer Reviewed
• [Journal Article] Anti-ice nucleation activity in xylem extracts from trees that contain deep supercooling xylem parenchyma cells (2007)
  • Author(s): Kasuga, Jun
  • Journal Title: Cryobiology 55 Pages: 305-314
  • NAID: 120000951625
  • Peer Reviewed
• [Presentation] 可溶性糖の添加によるブナ木部柔細胞の過冷却能力の変化 (2009)
  • Author(s): 春日純
  • Organizer: 第59回日本木材学会大会
  • Place of Presentation: 松本
  • Year and Date: 2009-03-14
• [Presentation] 可溶性糖の添加によるブナ木部柔細胞の過冷却能力の変化 (2009)
  • Author(s): 春日純
  • Organizer: 第59回日本木材学会大会
  • Place of Presentation: 松本, 松本大学
  • Year and Date: 2009-03-14
• [Presentation] 北方落葉広葉樹の凍結適応機構の解明-木部柔細胞に蓄積する過冷却促進成分の探索- (2008)
  • Author(s): 春日純
  • Organizer: 第53回日本植物学会北海道支部大会
  • Place of Presentation: 札幌
  • Year and Date: 2008-09-19
• [Presentation] 北方落葉広葉樹の凍結適応機構の解明-木部柔細胞に蓄積する過冷却促進成分の探索- (2008)
  • Author(s): 春日純
  • Organizer: 第53回日本植物学会北海道支部大会
  • Place of Presentation: 札幌,北海道大学
  • Year and Date: 2008-09-19
• [Presentation] カツラ木部柔細胞の深過冷却機構における過冷却促進フラボノイドの役割 (2008)
  • Author(s): 春日純
  • Organizer: 第58回日本木材学会大会
  • Place of Presentation: つくば
  • Year and Date: 2008-03-18
• [Presentation] カツラ木部柔細胞の深過冷却機構における過冷却促進フラボノイドの役割 (2008)
  • Author(s): 春日 純
  • Organizer: 第58回日本木材学会大会
  • Place of Presentation: つくば国際会議場,つくば
  • Year and Date: 2008-03-18
• [Presentation] Purification and identification of anti-ice nucleation substances in deep supercooling xylem parenchyma cells of katsura tree (Cercidiphyllum japonicum) (2008)
  • Author(s): JUN KASUGA
  • Organizer: PlantBiology 2008(2008 年度米国植物生物学会年会)
  • Place of Presentation: メリダ(メキシコ)
• [Presentation] Purification and identification of anti-ice nucleation substances in deep supercooling xylem parenchyma cells of katsura tree (Cercidiphyllum japonicum) (2008)
  • Author(s): Kasuga, Jun
  • Organizer: Plallt Biology 2008(2008年度米国植物生物学会年会)
  • Place of Presentation: メリダ(メキシコ), SIGLOXXI
• [Presentation] Identification and characterization of anti-ice nucleation substances in deep supercooling xylem parenchyma cells of Cercidiphyllum japonicum (2007)
  • Author(s): JUN KASUGA
  • Organizer: 8^th International Plant Cold Hardiness Seminar(第8回国際低温適応会議)
  • Place of Presentation: サスカチューン(カナダ)
  • Year and Date: 2007-08-08
• [Presentation] Identification and characterization of anti-ice nucleation substances in deep supercooling xylem parenchyma cells of Cercidiphyllum japonicum (2007)
  • Author(s): Kasuga, Jun
  • Organizer: 8th Internatioal Plant Cold Hardiness Seminar(第8回国際植物低温適応会議)
  • Place of Presentation: サスカチュワン大学,サスカチューン,カナダ
  • Year and Date: 2007-08-08
• [Book] Chapter 4 : Factors Related to Change of Deep Supercooling Capability in Xylem (eds L Gusta, M Wisniewski, K Tanino) (2009)
  • Author(s): SEIZO FUJIKAWA, JUN KASUGA, NAOKITAKATA, KEITA ARAKAWA
  • Publisher: CAB International, Plant Cold Hardiness : From the Laboratory to the Field(印刷中)
• [Book] Plant Cold Hardiness : From the Laboratory to the Field (eds L. Gusta, M. Wisniewski and K. Tanino), Chapter 4 : Factors Related to Change of Deep Supercooling Capability in Xylem Parenchyma Cells of Trees (2009)
  • Author(s): Fujikawa, Seizo
  • Publisher: CAB International(印刷中)
• [Remarks]
  • URL: http://www.agr.hokudai.ac.jp/woosci/woobio/index.html
• [Remarks]
  • URL: http://www.agr.hokudai.acjp/woosci/woobio/index.html
• [Remarks]
  • URL: http://www.agr.hokudai.ac.jp/woosci/woobio/index.html
• [Patent(Industrial Property Rights)] 過冷却促進剤 (2007)
  • Inventor(s): 藤川清三, 春日純, 橋床泰之, 荒川圭太, 福士幸治
  • Industrial Property Rights Holder: 北海道大学、オリンパス株式会社、藤川清三、春日純、橋床泰之、荒川圭太、福士幸治
  • Filing Date: 2007-07-11
  • Overseas
• [Patent(Industrial Property Rights)] 過冷却促進剤 (2007)
  • Inventor(s): 藤川清三, 春日純, 橋床泰之, 荒川圭太, 福士幸治
  • Industrial Property Rights Holder: 北海道大学, オリンパス株式会社
  • Filing Date: 2007-11-07
  • Overseas