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Development of system to establish canine embryonic stem cell line

Research Project

Project/Area Number 19880026
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Clinical veterinary science
Research InstitutionOsaka Prefecture University

Principal Investigator

HATOYA Shingo  Osaka Prefecture University, 大学院・生命環境科学研究科, 助教 (40453138)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,125,000 (Direct Cost: ¥2,720,000、Indirect Cost: ¥405,000)
Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000)
Fiscal Year 2007: ¥1,370,000 (Direct Cost: ¥1,370,000)
Keywordsイヌ / ES細胞 / 再生獣医療 / 体外受精 / 卵子
Research Abstract

卵巣から得られる、前胞状卵胞由来未成熟卵子を用いて、新たなイヌ体外受精技術の開発を試みた。(1)コラーゲン・ゲルの中にイヌ未成熟卵子を包埋した三次元培養法で長期間の培養が可能となり、未成熟なイヌ卵子を成長させることに成功した。(2)イヌES様細胞の初代コロニー形成に成功した。また、10代目までES様細胞を培養できることが分かった。しかしながら、早期の胚からイヌES様細胞の初代コロニーを形成することはできなかった。(3)イヌ未成熟卵子をエストロジェンおよび上皮成長因子を添加した培地で培養したところ、体外成熟が促進されることが明らかとなった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (6 results)

All 2009 2008

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] Canine oocyte maturation in culture : Significance of estrogen and EGF receptor gene expression in cumulus cells2009

    • Author(s)
      Hatoya S, Sugiyama Y, Nishida H, Okuno T, Torii R, Sugiura K, Kida K, Kawate N, Tamada H, Inaba T
    • Journal Title

      Theriogenology 71(4)

      Pages: 560-7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Canine oocyte maturation in culture : significance of estrogen and EGF receptor qene expression in cumulus cells.2009

    • Author(s)
      Hatoya S, et al.
    • Journal Title

      Theriogenology 71(4)

      Pages: 560-567

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of an expression vector for improved secretion of canine IL-182008

    • Author(s)
      Sugiura K, Akazawa T, Fujimoto M, Wijewardana V, Mito K, Hatoya S, Taketani S, Komori M, Inoue N, Inaba T
    • Journal Title

      Vet Immunol Immunopathol 126(3-4)

      Pages: 388-91

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Construction of an expression vector for improved secretion of canine IL-18.2008

    • Author(s)
      Sugiura K, et al.
    • Journal Title

      Vet Immunol Immunopathol. 126(3-4)

      Pages: 388-391

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] コラーゲン・ゲルを用いたイヌ卵母細胞の体外発育2009

    • Author(s)
      鳩谷晋吾、宮武希衣、大村雅、杉浦喜久弥、喜田加世子、川手憲俊、玉田尋通、稲葉俊夫
    • Organizer
      第134回日本生殖医学会関西支部集談会
    • Place of Presentation
      大阪
    • Year and Date
      2009-03-14
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] コラーゲン・ゲル培養イヌ卵母細胞の体外発育に及ぼすhypoxanthineとFSHの影響2008

    • Author(s)
      宮武希衣、鳩谷晋吾、奥野剛、鳥居隆三、杉浦喜久弥、喜田加世子、川手憲俊、玉田尋通、稲葉俊夫
    • Organizer
      日本獣医学会第146回大会
    • Place of Presentation
      宮崎
    • Year and Date
      2008-09-25
    • Related Report
      2008 Annual Research Report 2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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