イノシトール3リン酸受容体の制御を介した哺乳動物卵子の体外培養法・活性化法の開発

• Project/Area Number: 19880030
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: Azabu University
• Principal Investigator: ITO Junya Azabu University, 獣医学部, 准教授 (30454143)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,115,000 (Direct Cost: ¥2,710,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,360,000 (Direct Cost: ¥1,360,000)
• Keywords: 卵 / 受精 / イノシトール3リン酸受容体 / イノシトール3リン酸 / Plk1 / ブタ / 卵子 / 活性化

Research Abstract

動物に関係なく受精時に認められる卵内Ca^(2+)イオンの上昇, その中でも哺乳類の受精時特有な現象であるCa^(2+)反復上昇の制御に関して, 貯蔵されたCa^(2+)を放出させるイノシトール3リン酸受容体(IP_3R1)のリン酸化に焦点を当て, その結果マウス卵においてはPlk1が, ブタ卵においてはCDK1がそれぞれIP_3R1のリン酸化に重要な役割を果たしていることを初めて明らかにした.

Research Products

• [Journal Article] MG132 during cloning improves survival and pronucler number of reconstructed ratembryos (2008)
  • Author(s): Nakajima N, Inomata T, Ito J. et.al.
  • Journal Title: Cloning and Stem Cells 10 Pages: 461-468
  • Peer Reviewed
• [Journal Article] Inositol 1, 4, 5-triphosphate receptor 1, a widespread Ca^<2+> channel, is a novel substrate of polo-like kinase 1 in eggs (2008)
  • Author(s): Ito J., et.al.
  • Journal Title: Developmental Biology 320 Pages: 402-413
  • Peer Reviewed
• [Journal Article] Expression and immunodetection of Aquaporin 1 (AQP1) in canine spermatozoa (2008)
  • Author(s): Ito J., et.al.
  • Journal Title: Cryobiology 57 Pages: 312-314
  • Peer Reviewed
• [Journal Article] Inositol 1, 4, 5-triphosphate receptor 1, a widespread Ca^<2+>channel, is a novel substrate of polo-like kinase 1 in eggs (2008)
  • Author(s): Ito J., et al.
  • Journal Title: Developmental Biology 320 Pages: 402-413
  • Peer Reviewed
• [Journal Article] Expression and immunodetection of aquaporin 1 (AQP1)in canine spermatozoa (2008)
  • Author(s): Ito J., et al.
  • Journal Title: Cryobiology 57 Pages: 312-314
  • Peer Reviewed
• [Journal Article] Treatment with proteasome inhibitor MG132 during cloning improves survival and pronuclear number of reconstructed rat embryos (2008)
  • Author(s): Nakajima N., et al.
  • Journal Title: Cloning and Stem Cells 10 Pages: 461-468
  • Peer Reviewed
• [Journal Article] Effect of enucleation on inactivation of cytostatic factor activity in matured oocytes (2007)
  • Author(s): Ito J., et.al.
  • Journal Title: Cloning and Stem Cells 9 Pages: 257-266
  • Peer Reviewed
• [Journal Article] Effect of enucleation on inactivation of cytostatic factor activity in matured oocytes (2007)
  • Author(s): Ito J., et. al.
  • Journal Title: Cloning and Stem Cells 9 Pages: 257-266
  • Peer Reviewed
• [Journal Article] Phosphorylation of inositol 1, 4, 5-triphosphate receptor 1 during in vitro maturation of porcine oocytes
  • Author(s): Ito J., et.al.
  • Journal Title: Animal Science Journal (in press)
  • NAID: 10027888417
• [Presentation] Stage-dependent changes of IP_3R1 phosphorylation during in vitro maturation and development in pig oocytes (2009)
  • Author(s): Ito J., et.al.
  • Organizer: 34^<th> Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: アメリカ合衆国コロラド州デンバー
• [Presentation] Involvement of Calmodulin-dependent phosphodiesterase (PDE1) in canine spermatozoa (2008)
  • Author(s): Ito J., et al.
  • Organizer: 13^<th> AAAP
  • Place of Presentation: ハノイ(ベトナム)
• [Presentation] Stage-dependent changes of IP_3R1 phosphorylation during in vitro maturation and development in pig oocytes (2008)
  • Author(s): Ito J., et. al.
  • Organizer: 34th Annual conference of the international embryo transfer society
  • Place of Presentation: デンバー(アメリカ合衆国)