Production of polymer-based material from tannins by microbial function

• Project/Area Number: 19880035
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: 林産科学・木質工学
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: OTSUKA Yuichiro Forestry and Forest Products Research Institute, バイオマス化学研究領域, 研究員 (80455261)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,102,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥402,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000); Fiscal Year 2007: ¥1,360,000 (Direct Cost: ¥1,360,000)
• Keywords: タンニン / カテキン / バイオマス / 代謝工学 / Burkholderia / 土壌細菌 / 代謝機能 / Tannin / Catechin / Burkkholderia / Tropical peat / Leucocyanidin / Taxifolin / metabolic engineering / Biomass

Research Abstract

樹皮タンニンの構成単位であるカテキン類を炭素源として資化できる微生物を酸性土壌より単離し、Burkholderia sp. OX-01と命名した。代謝機能解析の結果、OX-01はカテキンの4位を水酸化してロイコシアニジンに変換した後、さらに同部位を酸化してタキシフォリンに変換した後、完全代謝する新規代謝機能により分解することが明らかとなった。また、OX-01株の詳細同定を行った結果、新種であることが明らかとなりBurkholderia oxyphila OX-01を提唱した。

Research Products

• [Journal Article] Biotransformation of (+)-catechin into taxifolin by a two-step oxidation: Primary stage of (+)-catechin metabolism by a novel (+)-catechin-degrading bacteria, Burkholderia sp. KTC-1, isolated from tropical peat (2008)
  • Author(s): Motoki Matsuda, Yuichiro Otsuka, Shigeki Jin, Jun Wasaki, Jun Watanabe, Toshihiro Watanabe, Mitsuru Osaki
  • Journal Title: Biochemical and Biophysical Research Communications 366 Pages: 414-419
  • Peer Reviewed
• [Journal Article] Biotransformation of (+)-catechin into taxifolin by a two-step oxidation: Primary stage of (+)-catechin metabolism by a novel (+)-catechin-degrading bacteria, Burkholderia sp. KTC-1, isolated from tropical peat. (2008)
  • Author(s): Motoki Matsuda
  • Journal Title: Biochemical and Biophysical Research Communications 366 Pages: 414-419
  • Peer Reviewed
• [Presentation] Burkholderia sp. nov., 熱帯酸性土壌由来の芳香族化合物代謝細菌 (2009)
  • Author(s): 大塚祐一郎、村田仁、松田元規、大崎満、中村雅哉
  • Organizer: 日本農芸化学会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-03-28
• [Presentation] Burkholderia sp. Nov., 熱帯酸性土壌由来の芳香族化合物代謝細菌 (2009)
  • Author(s): 大塚祐一郎, 村田仁, 松田元規, 大崎満, 中村雅哉
  • Organizer: 2009年度日本農芸化学会大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2009-03-28
• [Presentation] Isolation of Novel (+)-Catechin Degrading Bacterium, Burkholderia sp. KTC-1, from Tropical Peat and Detection of Taxifolin as Intermediate of (+)-Catechin Metabolism (2008)
  • Author(s): Yuichiro Otsuka, Motoki Matsuda, Masaya Nakamura, Shigeki Jin, Jun Wasaki, Jun Watanabe, Toshihiro Watanabe, Mitsuru Osaki
  • Organizer: American Society for Microbiology General Meeting
  • Place of Presentation: Boston Convention & Exhibition Center. Boston, MA
  • Year and Date: 2008-06-03
• [Presentation] Isolation of Novel (+)-Catechin Degrading Bacterium, Burkholderia sp. KTC-1, from Tropical Peat and Detection of Taxifolin as Intermediate of (+)-Catechin Metabolism (2008)
  • Author(s): Yuichiro Otsuka, Motoki Matsuda, Masaya Nakamura, Sigeki Jin, Jun Wasaki, Jun Watanabe, Toshihiro Watanabe, Mitsuru Osaki
  • Organizer: American Society for Microbiology General Meeting
  • Place of Presentation: Boston
  • Year and Date: 2008-06-02
• [Presentation] 熱帯泥炭より単離した新規(+)-catechin資化細菌Burkholderia sp. KTC-1による(+)-catechinのtaxifolinへの変換 (2008)
  • Author(s): 松田元規、大塚祐一郎、神繁樹、和崎淳、渡辺純、渡部敏裕、大崎満
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名古屋名城大学
  • Year and Date: 2008-03-28
• [Presentation] 熱帯泥炭より分離した新規(+)-catechin資化細菌Burkholderia sp.KTC-1による(+)-catechinのtaxifolinへの変換 (2008)
  • Author(s): 松田 元規
  • Organizer: 日本農芸化学会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28