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Studies on subseafloor microorganisms based on genes and metabolic pathways

Research Project

Project/Area Number 19880041
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

MIYAZAKI Junichi  Japan Agency for Marine-Earth Science and Technology, 極限環境生物圏研究センター, 研究員 (50435848)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,125,000 (Direct Cost: ¥2,720,000、Indirect Cost: ¥405,000)
Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000)
Fiscal Year 2007: ¥1,370,000 (Direct Cost: ¥1,370,000)
Keywords海底下微生物 / 安定同位体 / 微生物代謝活動 / メタゲノム
Research Abstract

本研究は地球最大の微生物圏である海底下微生物圏において生息する微生物のエネルギー代謝機構の解明を安定同位体を用いた取り込み実験によって行うものである。
そしてこれとフォスミド作成に適したDNA 抽出系と組み合わせることによって将来のメタゲノム解析に役立てていきたい。
初年度は海底堆積物からフォスミドを作成するのに最適な抽出系を構築した。
次年度は安定同位体を添加して海底堆積物を培養した。現在も培養を続けており、今後はここからDNA を抽出し、海底下微生物はどのようにエネルギー代謝を行っていくのかを明らかにしていきたい。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (13 results)

All 2009 2008 2007 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (11 results)

  • [Journal Article] 泥火山に生息する生物とその代謝活動2009

    • Author(s)
      宮崎淳一
    • Journal Title

      地学雑誌 118巻3号(印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 泥火山に生息する生物とその代謝活動

    • Author(s)
      宮崎淳一
    • Journal Title

      地学雑誌 118 巻3 号

      Pages: 0-0

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] Culture-dependent and-independent analysis of two hydrothermal fields in central Indian ridge2008

    • Author(s)
      Junichi Miyazaki
    • Organizer
      International Symposium of Subsurface Microbiology
    • Place of Presentation
      静岡市・グランシップ
    • Year and Date
      2008-11-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] Culture -dependent and -independent analysis of two hydrothermal fields in central Indian ridge2008

    • Author(s)
      Junichi Miyazaki
    • Organizer
      International Symposium of Subsurface Microbiology
    • Place of Presentation
      静岡市・グランシップ
    • Year and Date
      2008-11-17
    • Related Report
      2008 Final Research Report
  • [Presentation] Microbial communities of two deep-sea hydrothermal fields in Central Indian Ridge2008

    • Author(s)
      Junichi Miyazaki
    • Organizer
      International Symposium of Microbial Ecology
    • Place of Presentation
      オーストラリア・ケアンズ・ケアンズコンベンションセンター
    • Year and Date
      2008-08-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] Microbial communities of two deep-sea hydrothermal fields in Central Indian Ridge2008

    • Author(s)
      Junichi Miyazaki
    • Organizer
      8~<th> International Symposium of Microbial Ecology
    • Place of Presentation
      オーストラリア・ケアンズコンベンションセンター
    • Year and Date
      2008-08-18
    • Related Report
      2008 Final Research Report
  • [Presentation] Nitrogen fixation by anaerobic methanotroph. International symposium on environmental microbiology2008

    • Author(s)
      Junichi Miyazaki
    • Organizer
      -How do microbes control nutrient cycling in anaerobic environment-
    • Place of Presentation
      首都大学東京
    • Year and Date
      2008-02-28
    • Related Report
      2008 Final Research Report
  • [Presentation] Nitrogen fixation by anaerobic methanotroph2008

    • Author(s)
      宮崎 淳一
    • Organizer
      International Symposium on Environmental Microbiology
    • Place of Presentation
      首都大学東京
    • Year and Date
      2008-02-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] メタン冷湧水帯における窒素固定菌の分布2007

    • Author(s)
      宮崎淳一
    • Organizer
      第23回日本微生物生態学会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2007-09-17
    • Related Report
      2008 Final Research Report
  • [Presentation] メタン冷湧水帯における窒素固定菌の分布2007

    • Author(s)
      宮崎 淳一
    • Organizer
      微生物生態学会
    • Place of Presentation
      愛媛大学
    • Year and Date
      2007-09-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] Nitrogen fixation in anoxic methane-seep sediments2007

    • Author(s)
      Junichi Miyazaki
    • Organizer
      Gordon Research Conferences-Archaea; Ecology, Metabolism & Molecular Biology-
    • Place of Presentation
      Andover, NH, USA
    • Year and Date
      2007-08-23
    • Related Report
      2008 Final Research Report
  • [Presentation] Nitrogen fixation in anoxic methane-seep sediments. Gordon Research Conferences -Archaea; Ecology2007

    • Author(s)
      Junichi Miyazaki
    • Organizer
      Metabolism &Molecular Biology-
    • Place of Presentation
      Andover, NH, USA
    • Year and Date
      2007-08-20
    • Related Report
      2008 Final Research Report
  • [Presentation] Anaerobic methane-oxidizing archaea contribute not only to methane oxidizing but also to nitrogen fixation in cold-seep of the Kumano mud volcano2007

    • Author(s)
      Junichi Miyazaki
    • Organizer
      Gordon Research Conference-ARCHAEA:ECOLOGY, METABOLISM & MOLECULAR BIOLOGY-
    • Place of Presentation
      Proctor Academy, Andover, NH, USA
    • Year and Date
      2007-08-20
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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