The search for EMT-related genes using clinical specimens of the uterine carcinosarcoma

• Project/Area Number: 19890022
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: TOYOSHIMA Masafumi Tohoku University, 大学院・医学系研究科, 非常勤講師 (70451581)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,085,000 (Direct Cost: ¥2,680,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,330,000 (Direct Cost: ¥1,330,000)
• Keywords: 子宮癌肉腫 / 上皮間葉移行 / 間葉上皮移行 / 遠隔転移 / マイクロダイセクション / マイクロアレイ

Research Abstract

1.臨床標本について上皮間葉移行(EMT)に関わると考えられる分子Hrsの免疫染色を行った。免疫染色では関連は不明であったが、Hrsの中枢神経系での重要性を見出し論文発表した。
2.子宮癌肉腫症例標本をレーザーマイクロダイセクションにて癌と肉腫部分に分けてRNAを抽出した。しかし研究期間中に十分な症例数が集まらずアレイ解析は不可能であった。
3.低酸素環境とEMTの関連を解析した。子宮内膜癌細胞株を異なる酸素濃度下で培養し、低酸素濃度で間葉系細胞に類似する細胞形態変化とvimentinの増加を認めた。

Research Products

• [Journal Article] Generation of a syngeneic mouse model to study the intraperitoneal dissemination of ovarian cancer with in vivo luciferase imaging. (2009)
  • Author(s): Toyoshima M, Tanaka Y, Matumoto M, Yamazaki M, Nagase S, Sugamura K and Yaegashi N.
  • Journal Title: Luminescence (in press)
  • Peer Reviewed
• [Journal Article] Generation of a syngeneic mouse model to study the intraperitoneal dis semination of ovarian cancer with in vivo luciferase imaging (2009)
  • Author(s): Tovoshima M, Tanaka Y, Matumoto M, Yamazaki M, Nagase S, Sugamura K and Yaegashi N.
  • Journal Title: Luminescence (in press)
  • Peer Reviewed
• [Journal Article] Loss of Hrs in the Central Nervous System Causes Accumulation of Ubiquitinated Proteins and Neurodegeneration (2008)
  • Author(s): Tamai K, Toyoshima M, Tanaka N, Yamamoto N, Owada Y, Kiyonari H, Murata K, Ueno Y, Ono M, Shimosegawa T, Yaegashi N, Watanabe M, Sugamura K
  • Journal Title: Am J Pathol. 173(6) Pages: 1806-1817
  • Peer Reviewed
• [Journal Article] The therapeutic strategy for the uterine body cancer(in Japanese) (2008)
  • Author(s): Nobuo Yaegashi, Hitoshi Niikura, Masafumi Toyoshima
  • Journal Title: Focus on Oncology 8 Pages: 8-10
• [Journal Article] A diagnosis and treatment of the uterine body carcinoma.(in Japanese) (2008)
  • Author(s): Masafumi Toyoshima and Nobuo Yaegashi
  • Journal Title: Byori To Rinsho 26 Pages: 335-339
• [Journal Article] Loss of Hrs in the Central Nervous System Causes Accumulation of Ubiquitinated Proteins and Neurodegeneration (2008)
  • Author(s): Tamai K, Toyoshima M, Tanaka N, Yamamoto N, Owada Y, Kiyonari H, Murata K, Ueno Y, Ono M, Shimosegawa T, Yaegashi N, Watanabe M, Sugamura K
  • Journal Title: Am J Pathol 173(6) Pages: 1806-1817
  • Peer Reviewed
• [Journal Article] 子宮体がんに対する治療戦略 (2008)
  • Author(s): 八重樫伸生、新倉仁、豊島将文
  • Journal Title: Focus in oncology 8 Pages: 8-10
• [Journal Article] 子宮体癌の診断と治療 (2008)
  • Author(s): 豊島将文、八重樫伸生
  • Journal Title: 病理と臨床 26(4) Pages: 335-339
• [Journal Article] Inhibition of Tumor Growth and Metastasis by Depletion of Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate (Hrs), a Key Regulator of Monoubiquitinated Protein Sorting: Hrs-Mediated Regulation of E-Cadherin and B-Catenin (2007)
  • Author(s): Masafumi Toyoshima, Nobuyuki Tanaka, Jun Aoki, Yoshinori Tanaka, Kazuko Murata, Masanao Kyuuma, Hideyuki Kobayashi, Naoto Ishii, Nobuo Yaegashi, Kazuo Sugamura
  • Journal Title: Cancer Research 67 Pages: 5162-5171
  • Peer Reviewed
• [Presentation] 子宮癌肉腫の病態とそれを踏まえた治療戦略 (2008)
  • Author(s): 豊島将文
  • Organizer: 第44回日本婦人科腫瘍学会シンポジウム
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-07-17
• [Presentation] 子宮癌肉腫の病態とそれを踏まえた治療戦略 (2008)
  • Author(s): 豊島将文
  • Organizer: 日本婦人科腫瘍学会シンポジウム
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-07-17
• [Book] 婦人科がん標準化学療法の実際子宮肉腫癌肉腫 (2008)
  • Author(s): 豊島将文
  • Total Pages: 188
  • Publisher: 金原出版社