Regulatory mechanism of BMP-dependent osteoblast differentiation through TNF/JNK signaling pathway

• Project/Area Number: 19890027
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Tohoku University
• Principal Investigator: KUDO Tada-aki Tohoku University, 大学院・歯学研究科, 助教 (50431606)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,085,000 (Direct Cost: ¥2,680,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,330,000 (Direct Cost: ¥1,330,000)
• Keywords: BMP / TNF / MAPK / JNK / Dullard / 骨芽細胞分化

Research Abstract

BMP受容体の一つ、BMPRIIは、C末端領域のThr977がリン酸化酵素JNKによりリン酸化され、一方では脱リン酸化酵素Dullardにより分解の促進を受ける。本研究では、Dullardの機能とBMPRII部分欠失変異体との関係等や、Thr977がリン酸化を受けないBMPRII[T977A]変異体安定発現細胞が示す、野生型BMPRII安定発現細胞と異なる表現型についての新たな知見が得られた

Research Products

• [Journal Article] Bioinformatics-based cyclopedic search for novel JNK binding molecules (2007)
  • Author(s): T.A. Kudo, T. Kobayashi.& S. Tamura
  • Journal Title: Tohoku Univ.Dent.J. 26 Pages: 1-11
  • NAID: 110006606561
  • Peer Reviewed
• [Journal Article] Bioinformatics-based cyclopedic search for novel JNK binding molecules (2007)
  • Author(s): Tada-aki Kudo
  • Journal Title: Tohoku Univ. Dent. J 26 Pages: 1-11
  • NAID: 110006606561
  • Peer Reviewed
• [Presentation] Flp-In/T-RExシステムを応用したII型骨形成因子(BMP)受容体の新しい機能に関する網羅的解析 (2009)
  • Author(s): 工藤忠明, 清水良央, 張曄, 岡山啓昌, 趙菲, 狩野充浩, 田村眞理, 金高弘恭, 林治秀
  • Organizer: 第54回東北大学歯
  • Place of Presentation: 仙台市
  • Year and Date: 2009-02-27
• [Presentation] Flp-In/T-RExシステムを応用したII型骨形成因子(BMP)受容体の新しい機能に関する網羅的解析 (2009)
  • Author(s): 工藤忠明, 清水良央, 張曄, 岡山啓昌, 趙菲, 狩野充浩, ほか
  • Organizer: 第54回東北大学歯学会
  • Place of Presentation: 仙台市
  • Year and Date: 2009-02-27
• [Presentation] Establishment of a stable and regulable 293 cell line expressing the type II bone morphogenetic protein receptor : an approach toward proteome analysis (2009)
  • Author(s): T.A. Kudo, A. Watanabe, M. Asano, Y. Zhang, F. Zhao, M. Kano, Y. Shimizu, H. Kanetaka, S. Tamura, H. Hayashi
  • Organizer: The 3rd International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台市
• [Presentation] Regulation of the protein serine/threonine phosphatase Dullard in the course of neural differentiation of P19 embryonal carcinoma cells (2009)
  • Author(s): F. Zhao, T.A. Kudo, Y. Zhang, M. Kano, S. Tamura, Y. Shimizu, H. Kanetaka, H. Hayashi
  • Organizer: The 3rd International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台市
• [Presentation] Genome-wide analysis of the regulation of BMP2-induced gene expression by inflammatory cytokine TNF-a in a stable 293 cell line expressing BMPRII (2009)
  • Author(s): K. Okayama, T.A. Kudo, Y. Shimizu, Y. Zhang, F. Zhao, M. Kano, H. Kanetaka, K. Sasaki
  • Organizer: The 3rd International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台市
• [Presentation] Role of the extracellular-signal-regulated kinase 5(ERK5)pathway for neural differentiation in P19 embryonal carcinoma cells (2009)
  • Author(s): Y. Zhang, T.A. Kudo, M. Ku, F. Zhao, M. Kano, Y. Shimizu, H. Kanetaka, T. Hamada, H. Kanetaka
  • Organizer: The 3rd International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台市
• [Presentation] Dullard in the course of neural differentiation of P19 embryonal carcinoma cells (2009)
  • Author(s): F. Zhao, T. A. Kudo, Y. Zhang, M. Kano, et.al.
  • Organizer: The 3rd International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台市
• [Presentation] Role of the extracellular-signal-regulated kinase 5 (ERK5) pathway for neural differentiation in P19 embryonal carcinoma cells (2009)
  • Author(s): Y. Zhang, T. A. Kudo, M. Ku, F. Zhao, et.al.
  • Organizer: The 3rd International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: 仙台市
• [Presentation] Proton-associated sugar transporter-A is a potential target of JNK signal cascade (2008)
  • Author(s): 工藤忠明, 田村眞理, 林治秀
  • Organizer: 第5回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 横浜市
  • Year and Date: 2008-05-19
• [Presentation] Proton-associated sugar transporter-A is a potential target of JNK signal cascade (2008)
  • Author(s): 工藤忠明, 田村眞理, 林治秀
  • Organizer: 第5回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台市
  • Year and Date: 2008-05-19
• [Presentation] c-Jun N-terminal Kinase is a possible regulator of proton-associated sugar transporter-A (2008)
  • Author(s): 工藤忠明, 清水良央, 金高弘恭, 林治秀
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京都
• [Presentation] JNKによるproton-associated sugar transporter-Aの制御機構 (2008)
  • Author(s): 工藤忠明, 清水良央, 金高弘恭, 田村眞理, 林治秀
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜市
• [Presentation] JNKによるproton-associated sugar transporter-Aの制御機構 (2008)
  • Author(s): 工藤忠明, 清水良央, 金高弘恭, 田村眞理, 林治秀
  • Organizer: 第60回日本細胞生物学会
  • Place of Presentation: 横浜市
• [Presentation] 骨吸収性サイトカインによるBMPRIIの調節機構 (2007)
  • Author(s): 工藤忠明
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 札幌市
  • Year and Date: 2007-08-30
• [Presentation] 骨吸収性サイトカインによるBMPRIIの調節機構 (2007)
  • Author(s): 工藤忠明, 田村眞理, 林治秀
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌市
• [Book] 加齢医学エイジングファイン (2007)
  • Author(s): 小林孝安, 工藤忠明, 田村眞理. 帯刀益夫, 佐竹正延(編)
  • Publisher: 東北大学出版会