Augmentation of drug sensitivity in OSCC by epigenetical theory

• Project/Area Number: 19890028
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Tohoku University
• Principal Investigator: SUGAZAKI Masaki Tohoku University, 病院, 医員 (50444013)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,075,000 (Direct Cost: ¥2,670,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,320,000 (Direct Cost: ¥1,320,000)
• Keywords: エピジェネティクス / 口腔癌 / DNMT阻害剤 / アポトーシス

Research Abstract

以下の研究成果を得た。HSC-3細胞では、CDDP単独処理に比較してZEBの前処理またはSAHAの同時処理でCDDPによる細胞毒性が有意に増強した。CDDP単独で30%、CDDP/ZEBまたはCDDP/SAHAで80%のアポトーシス誘導が確認された。一方、5-FUの処理では、5-FU/SAHAでアポトーシス誘導の増強が観察されたが、5-FU/ZEBは逆に5-FUの細胞毒性を減弱するという、興味深い知見が得られた。このことから、口腔癌の抗癌剤感受性にはエピジェネティクス制御が関与し、ZEBやSAHAと抗癌剤の併用は薬剤感受性増強に有効であることが示唆された。

Research Products

• [Journal Article] Zebularine suppresses the apoptotic potential of 5-Fuorouracil via cAMP/PKA/CREB pathway against human oral squamous cell carcinoma cells (2009)
  • Author(s): Maiko Suzuki
  • Journal Title: Cancer Chemotherapy and Pharmacology (印刷中)
  • Peer Reviewed
• [Journal Article] Zebularine suppresses the apoptotic potential of 5-fluorouracil via cAMP/PKA/CREB pathway against human oral squamous cell carcinoma cells (2008)
  • Author(s): Suzuki M, Shinohara F, Endo M, Sugazaki M, Echigo S, Rikiishi H
  • Journal Title: Cancer Chemo. Pharma(Online Pub.)
  • Peer Reviewed
• [Journal Article] A case of clear cell carcinoma, not otherwise specified, in the retromolar region (2007)
  • Author(s): Okada Miwa
  • Journal Title: JAPANEZE JOURNAL OF ORAL & MAXILLOFACIAL SURJERY 53 Pages: 449-453
  • Peer Reviewed
• [Journal Article] A case of ameloblastoma in a 3-year-old child (2007)
  • Author(s): Izutsu Takashi
  • Journal Title: JAPANEZE JOURNAL OF ORAL & MAXILLOFACIAL SURJERY 53 Pages: 736-740
  • Peer Reviewed
• [Presentation] Effects of Zebularine on the apoptosis of 5-Fluorouracil via cAMP/PKA/CREB pathway against human oral squamous cell carcinoma cells (2009)
  • Author(s): Maiko Suzuki, Fumiaki Shinohara, Manabu Endo, Masaki Sugazaki, Seishi Echigo and Hidemi Rikiishi
  • Organizer: The 3^<rd> International Symposium for interface Oral Health Science
• [Presentation] 口腔癌の血管新生とエピジェネティクス制御 (2008)
  • Author(s): 鈴木麻衣子
  • Organizer: 歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-24
• [Presentation] 当科で手術を施行した高齢口腔癌症例の臨床的検討 (2007)
  • Author(s): Taniguchi Takahiro
  • Organizer: 第52回東北大学歯学会
  • Place of Presentation: 仙台
  • Year and Date: 2007-12-12