Development of a novel chamber applicable to various electrophysiological measurements

• Project/Area Number: 19890086
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: University of Fukui
• Principal Investigator: IWAMOTO Masayuki University of Fukui, 医学部, 助教 (40452122)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,075,000 (Direct Cost: ¥2,670,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,320,000 (Direct Cost: ¥1,320,000)
• Keywords: イオンチャネル / 脂質平面膜 / パッチクランプ

Research Abstract

本研究ではイオンチャネル蛋白質の機能を調べるための新しい電気生理測定系の開発を目指した。従来、測定対象とするイオンチャネルの存在環境(人工脂質膜中または細胞膜中など)によって異なる測定系を用いる必要があったが、新たに開発した測定方法ではこの制約を無くす事ができた。従って、多種多様なイオンチャネルに対し共通のプラットフォーム上での機能評価、およびイオンチャネルを標的とした創薬研究への応用が期待される。

Research Products

• [Journal Article] The water-ion coupling ratio for ion permeation through the KcsA potassium channel : Dependencies on concentration and species of permeating ions (2009)
  • Author(s): Iwamoto M, Shimizu H, Oiki S
  • Journal Title: Biophys.J 96 Pages: 179-179
• [Journal Article] The water-ion coupling ratio for ion permeation through the KcsA potassium channel : Dependencies on concentration and species of permeating ions (2009)
  • Author(s): Iwamoto, M.
  • Journal Title: Biophysical Journal 96 suppl.
• [Journal Article] Flux coupling between ion and water in the K+ selective pore (2008)
  • Author(s): Iwamoto M, Shimizu H, Oiki S
  • Journal Title: J.Physiol.Sci 58 Pages: 77-77
  • NAID: 130005449462
• [Journal Article] Kチャネルのイオン透過機構 : 新しい流動電位測定法により明らかになったイオン-水流束比 (2008)
  • Author(s): 老木成稔, 安藤博之, 久野みゆき, 清水啓史, 岩本真幸
  • Journal Title: 生物物理 48 Pages: 246-252
  • NAID: 110006839208
  • Peer Reviewed
• [Journal Article] Global twisting motion of singlemolecular KcsA potassium channelupon gating (2008)
  • Author(s): Shimizu H, Iwamoto M, Konno T, Nihei A, Sasaki YC, Oiki S
  • Journal Title: Cell 132 Pages: 67-78
  • Peer Reviewed
• [Journal Article] Kチャネルのイオン透過機構 : 新しい流動電位測定法により明らかになったイオン-水流束比 (2008)
  • Author(s): 老木成稔
  • Journal Title: 生物物理 48 Pages: 246-252
  • Peer Reviewed
• [Journal Article] Flux coupling between ion and water in the K+ selective pore (2008)
  • Author(s): Iwamoto M., et. al.
  • Journal Title: J. Physiol. Sci. 58(Suppl.)
  • NAID: 130005449462
• [Journal Article] Global twisting motion of single molecular KcsA potassium channel upon gating (2008)
  • Author(s): Shimizu H., et. al.
  • Journal Title: Cell 132 Pages: 67-78
  • Peer Reviewed
• [Presentation] The water-ion coupling ratio for ion permeation through the KcsA potassium channel: Dependencies on concentration and species of permeating ions (2009)
  • Author(s): Iwamoto M, Shimizu H, Oiki S
  • Organizer: Biophysical Society 53rd Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-03-01
• [Presentation] The water-ion coupling ratio for ion permeation through the KcsA potassium charmel : Dependencies on concentration and species of permeating ions (2009)
  • Author(s): Iwamoto, M.
  • Organizer: Biophysical Society 53^<rd> Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-03-01
• [Presentation] 流動電位測定によるKcsAカリウムチャネルのイオン透過機構の検討 (2008)
  • Author(s): 岩本真幸、清水啓史、老木成稔
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-05
• [Presentation] Flux coupling between ion and water in the K+ selective pore (2008)
  • Author(s): Iwamoto M, Shimizu H, Oiki S
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-25
• [Presentation] K+選択的透過におけるイオンと水のカップル比 (2008)
  • Author(s): 岩本 真幸
  • Organizer: 第85回日本生理学会大学
  • Place of Presentation: 東京
  • Year and Date: 2008-03-25
• [Presentation] Water-ion coupling ratios for ion permeation through KcsA channel (2008)
  • Author(s): Iwamoto M.
  • Organizer: Biophysical Society 52nd Annual Meeting
  • Place of Presentation: Long Beach, CA, USA
  • Year and Date: 2008-02-06
• [Presentation] Water-ion coupling ratios for ion permeation through KcsA channel (2008)
  • Author(s): Iwamoto M, Shimizu H, Oiki S
  • Organizer: Biophysical Society 52nd Annual Meeting
  • Place of Presentation: Long Beach, USA