Evaluation of donor lungs and their protection in the lung transplantation from donors after cardiac death

• Project/Area Number: 19890110
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: CHEN Fengshi Kyoto University, 医学研究科, 助教 (00452334)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,085,000 (Direct Cost: ¥2,680,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,330,000 (Direct Cost: ¥1,330,000)
• Keywords: 肺移植 / 心臓死 / 移植 / マージナルドナー / 臓器保護 / 吸入 / 体外回路

Research Abstract

「ドナー不足」に対する打開策として、心臓死ドナー(donation after cardiac death, DCD)肺を用いた肺移植がある。DCDでは心停止後の温虚血による臓器傷害が甚大であるが、吸入による薬物投与などの手法を開発することで、傷害肺の有効利用が可能になる。これに基づき、申請者は、以下一連の研究を行った。(1) rat肺ex vivo潅流モデルを用いて、さらに優れた肺保護作用を有する薬剤の検索(2)新しいDDSを用いた薬剤の吸入効率改善についての検討(3) 大動物ex vivo肺潅流モデルの確立。

Research Products

• [Journal Article] Nebulized phosphodiesterase III inhibitor during warm ischemia attenuates pulmonary ischemia-reperfusion injury (2009)
  • Author(s): Zhang J, Chen F, Zhao X, Aoyama A, Okamoto T, Fujinaga T, Shoji T, Sakai H, Cui Y, Bando T, Date H.
  • Journal Title: J Heart Lung Transplant 28(1) Pages: 79-84
  • Peer Reviewed
• [Journal Article] Protective effect of thioredoxin perfusion but not inhalation in warm ischemic-reperfused rat lungs (2009)
  • Author(s): Zhang J, Chen F, Nakamura T, Fujinaga T, Aoyama A, et al.
  • Journal Title: Redox Report
  • Peer Reviewed
• [Journal Article] Nebulized phosphodiesterase III inhibitor during warm ischemia attenuates pulmonary ischemia-reperfusion injury (2009)
  • Author(s): Zhang J, Chen F, Zhao X, Aoyama A, Okamoto T, Fujinaga T, et al.
  • Journal Title: J Heart Lung Transplant 28 (1) Pages: 79-84
  • Peer Reviewed
• [Journal Article] Value of FOXP3 expression in peripheral blood as rejection marker after miniature swine lung transplantation (2008)
  • Author(s): Satoda N, Shoji T, Wu Y, Fujinaga T, Chen F, Aoyama A, Zhang JT, Takahashi A, Okamoto T, Matsumoto I, Sakai H, Li Y, Zhao X, Manabe T, Kobayashi E, Sakaguchi S, Wada H, Ohe H, Uemoto S, Tottori J, Bando T, Date H, Koshiba T.
  • Journal Title: J Heart Lung Transplant 27(12) Pages: 1293-301
  • Peer Reviewed
• [Journal Article] Successful Subzero Nonfreezing Preservation of Rat Lungs at-2℃ with a New Supercooling Technology (2008)
  • Author(s): Okamoto T, Nakamura T, Zhang J, Aoyama A, Chen F, Fujinaga T, Shoji T, Hamakawa H, Sakai H, Manabe T, Wada H, Date H, Bando T.
  • Journal Title: J Heart Lung Transplant 27(10) Pages: 1150-7
  • Peer Reviewed
• [Journal Article] Potential for Pulmonary Protection by Nebulized Milrinone during Warm Ischemia (2008)
  • Author(s): Chen F, Zhang J, Aoyama A, Okamoto T, Fujinaga T, Bando T.
  • Journal Title: Transplant Proc 40(10) Pages: 3335-8
  • Peer Reviewed
• [Journal Article] Expression of endothelial specific adhesion molecules after cardiac arrest (2008)
  • Author(s): Chen F, Kondo N, Sonobe M, Fujinaga T, Wada H, Bando T.
  • Journal Title: Interact Cardiovasc Thorac Surg 7(3) Pages: 437-40
  • Peer Reviewed
• [Journal Article] Expression of endothelial specific adhesion molecules after cardiac arrest. (2008)
  • Author(s): Chen F, Kondo N, Sonobe M, Fujinaga T, Wada H, Bando T.
  • Journal Title: Int Cardiovasc Thorac Surg
  • Peer Reviewed
• [Journal Article] Protective effect of thioredoxin perfusion but not inhalation in warm ischemic-reperfused rat lungs
  • Author(s): Zhang J, Chen F, Nakamura T, Fujinaga T, Aoyama A, Hamakawa H, Sakai H, Hoshino Y, Yodoi J, Wada H, Bando T, Nakamura H.
  • Journal Title: Redox Report accepted
  • Peer Reviewed
• [Presentation] わが国のDCDからの肺移植実験に向けての実験的取り組み (2008)
  • Author(s): 岡本俊宏、陳豊史、藤永卓司、張吉天、青山晃博、趙向東、高橋鮎子、里田直樹、山田徹、森川洋匡、庄司剛、阪井宏彰、松本いずみ、板東徹、伊達洋至
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2008-09-21
• [Presentation] 心臓死ドナーからの肺移植に向けた、ドナー肺の評価とその保護の研究 (2008)
  • Author(s): 板東徹、陳豊史、藤永卓司、張吉天、青山晃博、岡本俊宏、趙向東、高橋鮎子、里田直樹、庄司剛、阪井宏彰、伊達洋至
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2008-09-21
• [Presentation] 心臓死ドナーからの肺移植に向けた、ドナー肺の評価とその保護の研究 (2008)
  • Author(s): 陳豊史、藤永卓司、張吉天、青山晃博、岡本俊宏、趙向東、高橋鮎子、里田直樹、庄司剛、阪井宏彰、板東徹
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎市(WS7-2)
  • Year and Date: 2008-05-16
• [Presentation] Novel superiority of subzero non-freezing preservation of rat lung with supercooling technology (2008)
  • Author(s): T. Okamoto, T. Nakamura, X. Zhao, J. Zhang, A. Aoyama, N. Satoda, A. Takahashi, F. Chen, T. Fujinaga, H. Hamakawa, T. Shoji, H. Sakai, T. Bando, and H Date
  • Organizer: 38th Annual Congress of the Korean Society for Transplantation and 7th Korea-Japan Transplantation Forum Oct 10
  • Place of Presentation: Seoul, Korea
• [Presentation] Novel superiority of subzero non-freezing preservation of rat lung with supercooling technology in ex vivo model and lung transplantation model (2008)
  • Author(s): Okamoto T, Zhao X, Nakamura T, Zhang J, Aoyama A, Satoda N, Takahashi A, Chen F, Fujinaga T, Hamakawa H, Shoji T, Sakai H, Bando T, Date H.
  • Organizer: 8th International Congress on Lung Transplantation
  • Place of Presentation: Paris
• [Presentation] Ex vivo evaluation of non-heart-beating donor lungs in larger-size pigs (2008)
  • Author(s): Okamoto T, Chen F, Zhang J, Zhao X, Satoda N, Takahashi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H.
  • Organizer: 8th International Congress on Lung Transplantation
  • Place of Presentation: Paris
• [Presentation] Experimental donor lungs from donation after cardiac death in various settings (2008)
  • Author(s): Yamada T, Chen F, Zhang J, Okamoto T, Fujinaga T, Morikawa H, Satoda N, Takahashi A, Shoji T, Sakai H, Bando T, Date H.
  • Organizer: 8th International Congress on Lung Transplantation
  • Place of Presentation: Paris
• [Presentation] Nanoparticle of cholesterol-bearing pullulan as a carrier of anticancer drug (2008)
  • Author(s): Sato K, Chen F, Aoyama A, Akiyoshi K, Date H.
  • Organizer: EACR 20-20th Meeting of the European Association for Cancer Research
  • Place of Presentation: Lyon, France
• [Presentation] Successful subzero non-freezing preservation of rat lungs with supercooling technology (2008)
  • Author(s): Okamoto T, Nakamura T, Zhang J, Aoyama A, Satoda N, Takahashi A, Zhao X, Chen F, Fujinaga T, Hamakawa H, Shoji T, Sakai H, Wada H, Bando T.
  • Organizer: Abstract#446, 28th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Boston, April
• [Presentation] Protective effect of nebulized milrinone in pulmonary warm ischemia-reperfusion injury (2008)
  • Author(s): Chen F, Zhang J, Zhao X, Aoyama A, Okamoto T, Fujinaga T, Takahashi A, Satoda N, Shoji T, Sakai H, Bando T.
  • Organizer: Abstract#142, 28th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Boston, April
• [Presentation] Protective effect of nebulized milrinone in pulmonary warm ischemia-reperfusion injury. (2008)
  • Author(s): Chen F, Zhang J, Zhao X, Aoyama A, Okamoto T, et al.
  • Organizer: 28th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: Boston, USA
• [Presentation] 肺温虚血再潅流傷害におけるミルリノン吸入の検討 (2007)
  • Author(s): 陳豊史、張吉天、青山晃博、岡本俊宏、藤永卓司、里田直樹、高橋鮎子、庄司剛、阪井宏彰、趙向東、松本泉、板東徹
  • Organizer: #216第43回日本移植学会総会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-11-24
• [Presentation] 肺温虚血再潅流傷害におけるミルリノン吸入の検討 (2007)
  • Author(s): 陳 豊史、張 吉天、青山晃博, ら
  • Organizer: 第43回日本移植学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2007-11-24
• [Presentation] 肺移植ドナープール拡大の試み (2007)
  • Author(s): 板東徹、陳豊史、藤永卓司、張吉天、趙向東、高橋鮎子、岡本俊宏、青山晃博、里田直樹、浜川博司、庄司剛、阪井宏彰、松本いずみ、和田洋巳
  • Organizer: 第34回日本臓器保存生物医学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2007-11-16
• [Presentation] 肺温虚血再潅流傷害におけるホスホジエステラーゼ阻害剤の吸入効果 (2007)
  • Author(s): 張吉天、陳豊史、趙向東、青山晃博、岡本俊宏、高橋鮎子、里田直樹、庄司剛、阪井宏彰、藤永卓司、板東徹
  • Organizer: 第34回日本臓器保存生物医学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2007-11-16
• [Presentation] 心停止後肺における心房利尿ペプチドの再灌流傷害抑制効果の検討 (2007)
  • Author(s): 青山晃博、藤永卓司、陳 豊史, ら
  • Organizer: 第23回日本呼吸器外科学会会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-05-18
• [Presentation] Perfusion but not inhalation of rhTRX ameliorates warmischemia reperfusion injury (2007)
  • Author(s): 張吉天, 藤永卓司, 陳 豊史, ら
  • Organizer: 第23回日本呼吸器外科学会会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-05-18
• [Presentation] Human atrial natriuretic peptide ameliorates warmischemia-reperfusion injury of the lung. (2007)
  • Author(s): Aoyama A, Chen F, Nakamura T, et. al.
  • Organizer: 27th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: San Francisco
  • Year and Date: 2007-04-26
• [Presentation] Human atrial natriuretic peptide ameliorates warm ischemia-reperfusion injury of the lung (2007)
  • Author(s): Aoyama A, Chen F, Nakamura T, Fujinaga T, Zhang JT, Okamoto T, Wada H, Bando T.
  • Organizer: Abstract, Abstract #, 27th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: San Francisco
• [Remarks]
  • URL: http://www.thoracic-kyoto-u.gr.jp/