Development of intracellular delivery/bio-imaging system using photoactivatable FRET dye

• Project/Area Number: 19890179
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Chemical pharmacy
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: UEDA Satoshi Gifu Pharmaceutical University, 薬学部, 助教 (50453056)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,075,000 (Direct Cost: ¥2,670,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,320,000 (Direct Cost: ¥1,320,000)
• Keywords: ペプデューシン / 膜透過 / ペプチド / プローブ

Research Abstract

パルミチン酸とGPCR細胞内ループ領域ペプチドのハイブリッド分子であるペプデューシンの膜透過機構の解明をめざした分子プローブの設計、合成および評価を行った。光切断とFRET蛍光クエンチングシステムを組み込んだモデルペプチドは、光照射による結合切断により、任意のタイミングで蛍光を復活させることができ、ペプデューシンの膜透過機構の解明に向けた有用なツールとなることが明らかとなった。

Research Products

• [Journal Article] Copper-Catalyzed Synthesis of Benzoxazoles via a Regioselective C-H Functionalization/C-O Bond Formation under Air Atmosphere (2009)
  • Author(s): S. Ueda, H. Nagasawa
  • Journal Title: J. Org. Chem. (in press)
  • Peer Reviewed
• [Journal Article] Synthesis of 2-Arylbenzoxazoles by Copper-Catalyzed Intramolecular Oxidative C-O Coupling of Benzanilides (2008)
  • Author(s): S. Ueda, H. Nagasawa
  • Journal Title: Angew. Chem. Int. Ed. Engl. 47(34) Pages: 6411-6413
  • Peer Reviewed
• [Journal Article] Identification of Novel Non-peptide CXCR4 antagonists by Ligand-based Design Approach (2008)
  • Author(s): S. Ueda, M. Kato, S. Inuki, H. Ohno, B. Evans, Zi-xuan Wang, S. C. Peiper, K. Izumi, E. Kodama, M. Matsuoka, H. Nagasawa, S. Oishi, N. Fujii
  • Journal Title: Bioorg. Med. Chem. Lett. 18(14) Pages: 4124-4127
  • Peer Reviewed
• [Journal Article] Synthesis and Application of Fluoresceinand Botin-labeled Molecular Probes for Chemokine Receptor CXCR4 (2008)
  • Author(s): Shinya Oishi, Ryo Masuda, Barry Evans, Satoshi Ueda, Yukiko Goto, Hiroaki Ohno, Akira Hirasawa, Gozo Tsujimoto, Zixuan Wang, Stephen C. Peiper, Eiichi Kodama, Masao Matsuoka, Nobutaka Fujii
  • Journal Title: ChemBioChem 9(7) Pages: 1154-1158
  • Peer Reviewed
• [Journal Article] Synthesis and Application of Fluorescein- and Biotin-Labeled Molecular Probes for the Chemokine Receptor CXCR4 (2008)
  • Author(s): Shinya Oishi
  • Journal Title: ChemBioChem 9 Pages: 1154-1158
  • Peer Reviewed
• [Journal Article] Synthesis and Application of Fluorescein-and Biotin-Labeled Molecular Probes for the Chemokine Receptor CXCR4 (2008)
  • Author(s): Shinya Oishi
  • Journal Title: Chem Bio Chem 9 Pages: 1154-1158
  • Peer Reviewed
• [Presentation] 銅触媒を用いた分子内酸化的C-Oカップリングによるオキサゾール環形成反応 (2009)
  • Author(s): 橋本翔太、上田聡, 岡田崇宏、奥田健介、永澤秀子
  • Organizer: 日本薬学会第128年回
  • Place of Presentation: 京都
• [Presentation] Copper-catalyzed C-H activation/C-O bond formation for the synthesis of benzoxazoles (2008)
  • Author(s): S. Ueda, H. Nagasawa
  • Organizer: The First International Symposium on Process Chemistry
  • Place of Presentation: Kyoto
• [Presentation] 銅触媒を用いた位置選択的C-H活性化/C-O結合形成によるベンゾオキサゾール環形成反応 (2008)
  • Author(s): 上田聡, 岡田崇宏、橋本翔太、奥田健介、永澤秀子
  • Organizer: 第34回反応と合成の進歩シンポジウム
  • Place of Presentation: 京都
• [Patent(Industrial Property Rights)] ベンゾオキサゾール化合物の製造方法 (2008)
  • Inventor(s): 永澤秀子、上田聡
  • Filing Date: 2008-04-14