Acute stress reaction in cerebral limbic system and brainstem

• Project/Area Number: 19890189
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: Osaka City University
• Principal Investigator: QUAN Li Osaka City University, 大学院・医学研究科, 助教 (60453130)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,039,000 (Direct Cost: ¥2,640,000、Indirect Cost: ¥399,000); Fiscal Year 2008: ¥1,729,000 (Direct Cost: ¥1,330,000、Indirect Cost: ¥399,000); Fiscal Year 2007: ¥1,310,000 (Direct Cost: ¥1,310,000)
• Keywords: ストレス反応 / 大脳辺縁系 / 脳幹 / 神経病理 / 神経化学 / エリスローポエチン / C反応性蛋白 / 受傷後生存時間 / ドーパミン / セロトニン / ノルアドレナリン

Research Abstract

法医鑑定では, 犯罪被害者の死因のみならず, 生存時間や受傷時の苦痛・身体的行動能力などについての医学的立証が求められることがある.ヒトの死の直前における神経伝達物質の中, 特にカテコラミンの測定を中心として, 致死的外傷(侵害刺激)が中枢神経系に及ぼす急性ストレス反応を生化学的に評価し, ヒト(法医剖検例)における脳組織, 脳脊髄液および血液内に残された痕跡を分子病理学, 分子生物学および神経化学・生理学的指標を用いて多元多角的に分析した.

Research Products

• [Journal Article] Analyses of cardiac blood cells and serum proteins with regard to cause of death in forensic autopsy cases (2009)
  • Author(s): Quan L, Ishikawa T, Michiue T, Li DR, Zhao D, Yoshida C, Chen JH, Komatsu A, Azuma Y, Sakoda S, Zhu BL, Maeda H.
  • Journal Title: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262983
• [Journal Article] Postmortem serum levels of pulmonary surfactant-associated proteins A and D with regard to the cause of death in medicolegal autopsy (2009)
  • Author(s): Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Journal Title: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262989
• [Journal Article] Immunohistochemistry of von Willebrand factor in the lungs with regard to the cause of death in forensic autopsy (2009)
  • Author(s): Quan L, Ishikawa T, Zhao D, Michiue T, Yoshida C, Chen JH, Wang Q, Zhu BL, Maeda H.
  • Journal Title: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262975
• [Journal Article] Quantitative analysis of single-stranded DNA immunoreactivity as a marker of neuronal apoptosis in hippocampus with regard to the causes of death in medicolegal autopsy (2009)
  • Author(s): Michiue T, Quan L, Ishikawa T, Zhu BL, Maeda H.
  • Journal Title: Leg Med(Tokyo) (Epub ahead of print)
  • NAID: 10027262572
• [Journal Article] Evaluation of postmortem calcium and magnesium levels in the pericardial fluid with regard to the cause of death in medicolegal autopsy (2009)
  • Author(s): Li DR, Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Journal Title: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262910
• [Journal Article] Postmortem mRNA quantification for investigation of infantile death : A comparison with adult cases (2009)
  • Author(s): Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatu A, Chen JH, Wang Q,Zhu BL, Maeda H.
  • Journal Title: Leg Med (Tokyo) (Epub ahead of print)
  • NAID: 10027262946
• [Journal Article] Postmortem quantitative mRNA analyses of death investigation in forensic pathology : An overview and prospects (2009)
  • Author(s): Zhao D, Ishikawa T, Quan L, Michiue T, Zhu BL, Maeda H.
  • Journal Title: Leg Med (Tokyo) [Epub ahead of print]
  • NAID: 10027261968
• [Journal Article] Evaluation of pulmonary GLUT1 and VEGF mRNA levels in relation to lung weight in medicolegal autopsy cases (2009)
  • Author(s): Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatu A, Chen JH, Wang Q, Zhu BL, Maeda H.
  • Journal Title: Leg Med [Epub ahead of print]
  • NAID: 10027262961
• [Journal Article] Immunohistochemical distribution of basic fibroblast growth factor (bFGF) in medicolegal autopsy (2009)
  • Author(s): Wang Q, Ishikawa T, Quan L, Zhao D, Li DR, Michiue T, Chen JH, Zhu BL, Maeda H.
  • Journal Title: Leg Med (Tokyo) [Epub ahead of print]
  • NAID: 10027262517
• [Journal Article] Significance of postmortem biochemistry in determining the cause of death (2009)
  • Author(s): Maeda H, Zhu BL, Ishikawa T, Quan L, Michiue T.
  • Journal Title: Leg Med [Epub ahead of print]
  • NAID: 10027261990
• [Journal Article] Analysis of postmortem biochemical findings with regard to the lung weight in drowning (2009)
  • Author(s): Maeda H, Zhu BL, Ishikawa T, Quan L, Michiue T, Bessho Y, Okazaki S, Kamikodai Y, Tsuda K, Komatsu A, Azuma Y.
  • Journal Title: Leg Med [Epub ahead of print]
  • NAID: 10027262889
• [Journal Article] Analyses of cardiac blood cells and serum proteins with regard to cause of death in forensic autopsy cases. (2009)
  • Author(s): Quan L, Ishikawa T, Michiue T, Li DR, Zhao D, Yoshida C, Chen JH, Komatsu A, Azuma Y, Sakoda S, Zhu BL, Maeda H.
  • Journal Title: Legal Med. (In press)
  • NAID: 10027262983
  • Peer Reviewed
• [Journal Article] Postmortem serum levels of pulmonary surfactant-associated proteins A and D with regard to the cause of death in medicolegal autopsy. (2009)
  • Author(s): Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Journal Title: Legal Med. (In press)
  • NAID: 10027262989
  • Peer Reviewed
• [Journal Article] Immunohistochemistry of von Willebrand factor in the lungs with regard to the cause of death in forensic autopsy. (2009)
  • Author(s): Quan L, Ishikawa T, Zhao D, Michiue T, Yoshida C, Chen JH, Wang Q, Zhu BL, Maeda H.
  • Journal Title: Legal Med. (In press)
  • NAID: 10027262975
  • Peer Reviewed
• [Journal Article] Quantitative analysis of single-stranded DNA immunoreactivity as a marker of neuronal apoptosis in hippocampus with regard to the causes of death in medicolegal autopsy. (2009)
  • Author(s): Michiue T, Quan L, Ishikawa T, Zhu BL, Maeda H.
  • Journal Title: Legal Med. (In press)
  • NAID: 10027262572
  • Peer Reviewed
• [Journal Article] Evaluation of postmortem calcium and magnesium levels in the pericardial fluid with regard to the cause of death in medicolegal autopsy. (2009)
  • Author(s): Li DR, Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Journal Title: Legal Med. (In press)
  • NAID: 10027262910
  • Peer Reviewed
• [Journal Article] Evaluation of pulmonary GLUT1 and VEGF mRNA levels in relation to lung weight in medicolegal autopsy cases. (2009)
  • Author(s): Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatu A, Chen JH, Wang Q, Zhu BL, Maeda H.
  • Journal Title: Legal Med. (In press)
  • NAID: 10027262961
  • Peer Reviewed
• [Journal Article] Postmortem serum erythropoietin levels in establishing the cause of death and survival time at medicolegal autopsy (2008)
  • Author(s): Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Li DR, Ogawa M, Maeda H.
  • Journal Title: Int J Legal Med 122(6) Pages: 481-7
  • Peer Reviewed
• [Journal Article] Postmortem biochemistry and immunohistochemistry of adrenocorticotropic hormone with special regard to fatal hypothermia (2008)
  • Author(s): Ishikawa T, Quan L, Li DR, Zhao D, Michiue T, Hamel M, Maeda H.
  • Journal Title: Forensic Sci Int 179(2-3) Pages: 147-51
  • Peer Reviewed
• [Journal Article] Single-stranded DNA as an immunohistochemical marker of neuronal damage in human brain : an analysis of autopsy material with regard to the cause of death (2008)
  • Author(s): Michiue T, Ishikawa T, Quan L, Li DR, Zhao D, Komatsu A, Zhu BL, Maeda H.
  • Journal Title: Forensic Sci Int 178(2-3) Pages: 185-91
  • Peer Reviewed
• [Journal Article] Suicidal vehicle-assisted ligature strangulation resulting in complete decapitation : Anautopsy report and a review of the literature (2008)
  • Author(s): Zhao D, Ishikawa T, Quan L, Li DR, Michiue T, Maeda H.
  • Journal Title: Leg Med 10(6) Pages: 310-5
  • Peer Reviewed
• [Journal Article] Immunohistochemical distribution of single-stranded DNA in the brain in medico-legal autopsy cases of carbon monoxide intoxication (2008)
  • Author(s): Michiue T, Ishikawa T, Quan L, Li DR, Komatsu A, Zhao D, Chen JH, Yoshida C, Ogawa M, Maeda H.
  • Journal Title: Chudoku Kenkyu 21(1) Pages: 63-8
  • Peer Reviewed
• [Journal Article] Tissue-specific differences in mRNA quantification of glucose transporter 1 and vascular endothelial growth factor with special regard to death investigations of fatal injuries (2008)
  • Author(s): Zhao D, Ishikawa T, Quan L, Li DR, Michiue T, Yoshida C, Komatu A, Chen JH, Zhu BL, Maeda H.
  • Journal Title: Forensic Sci Int 177(2-3) Pages: 176-83
  • Peer Reviewed
• [Journal Article] Forensic pathological evaluation of injury severity and fatal outcome in traffic accidents : five illustrative autopsy cases of clinically unexpected death (2008)
  • Author(s): Michiue T, Ishikawa T, Quan L, Zhu BL, Maeda H.
  • Journal Title: Forensic Sci Med Pathol 4(3) Pages: 153-8
  • Peer Reviewed
• [Journal Article] 片側性血胸に合併した対側性外傷性気胸による急死の1剖検例 (2008)
  • Author(s): 権力, 道上知美, 趙東, 李冬日, 石川隆紀, 前田均
  • Journal Title: 法医病理 (14) Pages: 22-28
  • Peer Reviewed
• [Journal Article] Postmortem serum erythropoietin levels in establishing the cause of death and survival time at medicolegal autopsy. (2008)
  • Author(s): Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Li DR, Ogawa M, Maeda H.
  • Journal Title: Int J Legal Med. 122 Pages: 481-487
  • Peer Reviewed
• [Journal Article] Postmortem biochemistry and immunohistochemistry of adrenocort icotropic hormone with special regard to fatal hypothermia. (2008)
  • Author(s): Ishikawa T, Quan L, Li DR, Zhao D, Michiue T, Hamel M, Maeda H.
  • Journal Title: Forensic Sci Int. 179 Pages: 147-151
  • Peer Reviewed
• [Journal Article] Single-stranded DNA as an immunohistochemical marker of neuronal damage in human brain : an analysis of autopsy material with regard to the cause of death. (2008)
  • Author(s): Michiue T, Ishikawa T, Quan L, Li DR, Zhao D, Komatsu A, Zhu BL, Maeda H.
  • Journal Title: Forensic Sci Int. 178 Pages: 185-191
  • Peer Reviewed
• [Journal Article] Suicidal vehicle-assisted ligature strangulation resulting in complete decapitation : An autopsy report and a review of the literature. (2008)
  • Author(s): Zhao D, Ishikawa T, Quan L, Li DR, Michiue T, Maeda H.
  • Journal Title: Legal Med. 10 Pages: 310-315
  • Peer Reviewed
• [Journal Article] 片側性血胸に合併した対側性外傷性気胸による急死の1剖検例. (2008)
  • Author(s): 権力, 道上知美, 趙東, 李冬日, 石川隆紀, 前田均.
  • Journal Title: 法医病理 14 Pages: 22-28
  • Peer Reviewed
• [Journal Article] Tissue-specific differences in mRNA quantification of glucose transporter 1 and vascular endothelial growth factor with special regard to death investigations of fatal injuries (2008)
  • Author(s): Dong Zhao, Takaki Ishikawa, Li Quan, Dong-Ri Li, Tomoni Michiue, Chiemi Yoshida, Ayumi Komatsu, Jian-Hua Chen, Bao-Li Zhu, Hitoshi Maeda
  • Journal Title: Forensic Science international 177 Pages: 176-183
• [Journal Article] Single-stranded DNA as an immunohistochemical marker of neuronal damage in human brain:An analysis of autopsy material with reard to the cause of death (2008)
  • Author(s): Tomomi Michiue, Takaki Ishikawa, Li Quan, Dong-Ri Li, Dong Zhao, Ayumi Komatsu, Bao-Li Zhu, Hitoshi Maeda
  • Journal Title: Forensic Science international (Accepted)
• [Presentation] Postmortem catecholamine levels in pericardial and cerebrospinal fluids with regard to the cause of death in medicolegal autopsy (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine, Sep, Jpn J Legal Med 2008
  • Place of Presentation: Osaka(62 :138)
• [Presentation] Postmortem serum erythropoietin and C-reactive Protein levels as markers of survival time in injury deaths (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine, Sep, Jpn J Legal Med 2008
  • Place of Presentation: Osaka(62 : 138-139)
• [Presentation] Postmortem catecholamine levels in pericardial and cerebrospinal fluids with regard to the cause of death in medicolegal autopsy. (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Postmortem serum erythropoietin and C-reactive Protein levels as markers of survival time in injury deaths. (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] lmmunohistochemistry of von Willebrand factor in the lungs with regard to the cause of death in forensic autopsy. (2008)
  • Author(s): Quan L, Ishikawa T, Zhao D, Michiue T, Yoshida C, Chen J-H, Wang Q, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Analyses of cardiac blood cells and serum proteins with regard to cause of death in forensic autopsy cases. (2008)
  • Author(s): Quan L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Komatsu A, Azuma Y, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Postmortem serum erythropoietin level with regard to cause of death and survival time in medicolegal autopsy. (2008)
  • Author(s): Ouan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Ogawa M, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Postmortem serum levels of pulmonary surfactant associated proteins A and D with regard to the cause of death in medicolegal autopsy. (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Postmortem serotonin levels in cerebrospinal and pericardial fluid with regard to the cause of death in medicolegal autopsy. (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Postmortem lung weight with regard to survival time. (2008)
  • Author(s): Chen J-H, Quan L, Ishikawa T, Michiue T, Wang Q, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Evaluation of postmortem calcium and magnesium levels in the pericardial fluid with regard to the cause of death in medicoleg al autopsy. (2008)
  • Author(s): Li D-R, Quan L, Zhu B-L, I shikawa T, Michiue T, Zhao D, Yoshida C, Komatsu A, Azuma Y, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Quantitative analysis of single stranded DNA immunoreactivity as a marker of neuronal apoptosis in hippocampus with regard to the causes of death in medicolegal autopsy. (2008)
  • Author(s): Michiue T, Quan L, Ishikawa T, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Evaluation of pulmonary GLUT1 and VEGF mRNA levels in relation to the lung weight in medicolegal autopsy cases. (2008)
  • Author(s): Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatsu A, Chen J-H, Wang Q, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
• [Presentation] Sudden death following a struggle during arrest by the police : four autopsy cases. (2008)
  • Author(s): Ishikawa T, Michiue T, Quan L, Zhu B-L, Maeda H.
  • Organizer: International Association of Forensic Sciences 18th Triennial Meeting
  • Place of Presentation: New Orleans, USA.
• [Presentation] 剖検例における血清エジスロポェチン濃度の分析. (2008)
  • Author(s): 権力, 朱宝利, 石川隆紀, 道上知美, 李冬日, 趙東, 吉田智美, 東洋子, 別所康守, 前田均
  • Organizer: 第92次日本法医学会総会
  • Place of Presentation: 長崎
• [Presentation] 薬毒物中毒死剖検例の血液・体液内カテコラミンおよびセロトニン濃度. (2008)
  • Author(s): 権力, 石川隆紀, 道上知美, 織谷茂樹, 小川雅史, 前田均.
  • Organizer: 第30回日本中毒学会総会・学術集会, 中毒研究2008 ; 21 : 439-440
  • Place of Presentation: 和歌山
• [Presentation] 剖検例における脳神経細胞のアポトーシスに関する1本鎖DNAを指標とした定量形態学的分析 (2007)
  • Author(s): 道上 知美, 石川 隆紀, 権 力, 李 冬日, 趙 東, 小松 亜由美, 朱 宝利, 前田 均
  • Organizer: 第91次日本法医学会総会
  • Place of Presentation: 秋田県秋田ビューホテル
• [Presentation] Postmortem cardiac blood hematology for investigation of acute cause of death in medicolegal autopsy (2007)
  • Author(s): Quan L, Ishikawa T, Li D-R, Zhao D, Michiue T, Chen J-H, Zhu B-L, Maeda H.
  • Organizer: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacific Association of Law, Medicine and Science
  • Place of Presentation: Sri Lanka
• [Presentation] Evaluation of postmortem serum and cerebrospiral fluid ACTH levels in relation to the cause of death in forensic autopsy (2007)
  • Author(s): Ishikawa T, Quan L, Li D-R, Zhao D, Michiue T, Hamel M, Maeda H.
  • Organizer: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacific Association of Law, Medicine and Science
  • Place of Presentation: Sri Lanka
• [Presentation] Neuronal ischemic change and apoptosis in the hippocampus after brain injury (2007)
  • Author(s): Li D-R, Ishikawa T, Quan L, Zhao D, Michiue T, Zhu B-L, Maeda H.
  • Organizer: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacifc Association of Law, Medicine and Science
  • Place of Presentation: Sri Lanka
• [Presentation] Tissue-specific patterns of glucose transporter 1 and vascular endoth elial growth factor mRNA expression in autopsy material with special regard to injury death (2007)
  • Author(s): Zhao D, Ishikawa T, Quan L, Li D-R, Michiue T, Zhu B-L, Maeda H.
  • Organizer: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacific Association of Law, Medicine and Science
  • Place of Presentation: Sri Lanka
• [Presentation] Immunohistochemical distribution of single stranded DNA in the brain in medico-legal autopsy cases of carbon monoxide intoxication (2007)
  • Author(s): Michiue T, Ishikawa T, Quan L, Li D-R, Zhao D, Komatsu A, Maeda H.
  • Organizer: 9^<th> Indo-Pacific Congress on Legal Medicine & Forensic Sciences of The Indo-Pacific Association of Law, Medicine and Science
  • Place of Presentation: Sri Lanka