Regulation of cell death by HRD1

• Project/Area Number: 19890238
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Aichi Gakuin University
• Principal Investigator: 伊藤 友香 Aichi Gakuin University, 薬学部, 助教 (40454326)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,841,000 (Direct Cost: ¥2,490,000、Indirect Cost: ¥351,000); Fiscal Year 2008: ¥1,521,000 (Direct Cost: ¥1,170,000、Indirect Cost: ¥351,000); Fiscal Year 2007: ¥1,320,000 (Direct Cost: ¥1,320,000)
• Keywords: 細胞死 / ストレス / カルシウムイオン / アポトーシス / スフィンゴシルホスホリルコリン / 発現制御 / シグナル伝達

Research Abstract

RA患者由来滑膜細胞株MH7Aを糖鎖付加阻害剤のツニカマイシン(Tun)あるいはCa2^<+->ATPase阻害剤のタプシガルギン(Tg)で刺激を行い、小胞体ストレスによる細胞死について検討した。両刺激とも濃度依存的に細胞死を誘導し、Caspase-9、Caspase-3の活性化を惹起した。HRD1高発現細胞株では、野生型と比較してTunによる細胞死は減少したがTgによる細胞死は増加した。HRD1高発現細胞株はTg誘導性Bim発現上昇に対して影響を与えなかったことから、Bim発現制御以外の機構で細胞死を制御していると考えられた。

Research Products

• [Journal Article] Regulation of vascular tone by purinoceptor-activation in vascular smooth muscle and endothelial cells (2008)
  • Author(s): Itoh Y, Hatano N, and Muraki K.
  • Journal Title: The Aichi Gakuin Journal of Pharmaceutical Sciences 1 Pages: 31-37
  • Peer Reviewed
• [Journal Article] TRPV4-Like Non-selective Cation Currents in Cultured Aortic Myocytes (2008)
  • Author(s): Tanaka R, Muraki K, Ohya S, Yamamura H, Hatano N, Itoh Y, and Imaizumi Y
  • Journal Title: Journal of Pharmacological Scencies 108 Pages: 179-189
  • Peer Reviewed
• [Journal Article] Cell-culture-dependent change of Ca2+ response of rat aortic myocytes to sphingosine-1-phosphate (2008)
  • Author(s): Tanaka R, Muraki K, Ohya S, Itoh Y, Hatano N, and Imaizumi Y.
  • Journal Title: Journal of Pharmacological Sciences 107 Pages: 434-442
  • Peer Reviewed
• [Journal Article] Cigarette smoke condensate upregulates the gene and protein expression of proinflammatory cytokines in human fibroblast-like synoviocyte line (2008)
  • Author(s): Shizu M, Itoh Y, Sunahara R, Chujo S, Hayashi H, Ide Y, Takii T, Koshiko M, Chung SW, Hayakawa K, Miyazawa K, Hirose K, and Onozaki K.
  • Journal Title: Journal of Interferon and Cytokine Reserch 28 Pages: 509-521
  • Peer Reviewed
• [Journal Article] H_2O_2はcGMP非依存的にPKGを活性化して平滑筋弛緩反応を惹起する (2008)
  • Author(s): 伊藤友香
  • Journal Title: ファルマシア 44 Pages: 568-569
  • Peer Reviewed
• [Journal Article] Cell-culture-dependent change of Ca2+ response of rat aortic myocytes to sphingosine-1-phosphate (2008)
  • Author(s): Tanaka R, et al
  • Journal Title: J. Pharmacol. Sci. 107 Pages: 434-442
  • Peer Reviewed
• [Journal Article] TRPV4-like non-selective cation currents in cultured aortic myocyte (2008)
  • Author(s): Tanaka R, et al
  • Journal Title: J. Pharmacol. Sci. 108 Pages: 179-189
  • Peer Reviewed
• [Journal Article] Regulation of vascular tone by purinoceptor-activation in vascular smooth muscle and endothelial cells (2008)
  • Author(s): Itoh Y, et al
  • Journal Title: AICHI-GAKUIN J. PHARMACEUT. SCI. 1 Pages: 31-37
  • Peer Reviewed
• [Journal Article] H_2O_2はcGMP非依存的にPKGを活性化して平滑筋弛緩反応を惹起する (2008)
  • Author(s): 伊藤友香
  • Journal Title: ファルマシア 44(印刷中)
  • Peer Reviewed
• [Journal Article] Cigarette smoke condensate up-regulates the gene and protein expression of proinflammatory cytokines in human fibroblast-like synoviocyte line
  • Author(s): M. Shizu, et. al.
  • Journal Title: Journal of Interferon & Cytokine Research (in press)
  • Peer Reviewed
• [Presentation] スフィンゴシルホスホリルコリンによる関節リウマチ滑膜細胞死の制御 (2009)
  • Author(s): 伊藤友香
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-27