The clarification of the regulatory mechanism of calcium dynamics during osteoclastogenesis

• Project/Area Number: 19890273
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KURODA Yukiko The Institute of Physical and Chemical Research, 発生神経生物研究チーム, 基礎科学特別研究員 (70455343)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,075,000 (Direct Cost: ¥2,670,000、Indirect Cost: ¥405,000); Fiscal Year 2008: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000); Fiscal Year 2007: ¥1,320,000 (Direct Cost: ¥1,320,000)
• Keywords: シグナル伝達 / 発生・分化 / 破骨細胞 / カルシウムシグナル

Research Abstract

申請者はIP3R結合タンパク質の一つであるIRBITをノックダウンすると破骨細胞分化因子RANKLによって誘導されるカルシウムオシレーションの始まるタイミングが早まる傾向があることを見いだした。この結果より、IRBITが破骨細胞分化誘導時のカルシウム動態に深く関わっている分子であることが明らかとなった。そこで、破骨細胞分化時、および未分化時にそれぞれ特異的にIRBITに結合するタンパク質のスクリーニングを行い、現在はその中の候補タンパク質の一つであるE3ユビキチンリガーゼに注目し、研究を進めている。

Research Products

• [Journal Article] Inositol 1,4,5-triphosphate receptor- binding protein released with inositol 1,4,5-triphosphate (IRBIT) associates with components of the mRNA 3' processing machinery in a phosphorylation-dependent manner and inhibits polyadenylation (2009)
  • Author(s): Kiefer H, Mizutani A, Iemura S, Natsume T, Ando H, Kuroda Y, Mikoshiba K
  • Journal Title: J. Biol. Chem. 17;284(16) Pages: 10694-705
  • Peer Reviewed
• [Journal Article] 80K-H interacts with inositol 1,4,5- trisphosphate (IP3) receptors and regulates IP3-induced calcium release activity (2009)
  • Author(s): Kawaai K, Hisatsune C, Kuroda Y, Mizutani A, Tashiro T, Mikoshiba K
  • Journal Title: J. Biol. Chem. 2;284(1) Pages: 372-80
  • Peer Reviewed
• [Journal Article] 80K-H interacts with inositol 1,4,5-trisphosphate (IP3) receptors and regulates IP3-induced calcium release activity (2009)
  • Author(s): Kawaai K., et al.
  • Journal Title: J. Biol. Chem. 284 Pages: 372-80
  • Peer Reviewed
• [Journal Article] Osteoblasts induce Ca^<2+> Oscillation- independent NFATc1 activation during Osteoclastogenesis (2008)
  • Author(s): Kuroda Y, Hisatsune C, Nakamura T, Matsuo K, Mikoshiba K
  • Journal Title: Proc. Natl. Acad. Sci. USA 24;105(25) Pages: 8643-8
  • Peer Reviewed
• [Journal Article] Phosphorylation of Homer3 by CaMKII regulates a coupling state of its target molecules in Purkinje Cells (2008)
  • Author(s): Mizutani A, Kuroda Y, Futatsugi A, Furuichi T, Mikoshiba K
  • Journal Title: J. Neurosci. 14;28(20) Pages: 5369-82
  • Peer Reviewed
• [Journal Article] Osteoblasts induce Ca2+ oscillation-independent NFATc1 activation during osteoclastogenesis (2008)
  • Author(s): Kuroda Y., et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 8643-8
  • Peer Reviewed
• [Journal Article] Phosphorylation of Homer3 by calcium/calmodulin-dependent kinase II regulates a coupling state of its target molecules in Purkinje cells (2008)
  • Author(s): Mizutani A., et al.
  • Journal Title: J. Neurosci. 28 Pages: 5369-82
  • Peer Reviewed
• [Journal Article] Abnormal taste perception in mice lacking the type 3 inositol 1,4,5-trisphosphate receptor (2007)
  • Author(s): Hisatsune C, Yasumatsu K, Takahashi- Iwanaga H, Ogawa N, Kuroda Y, Yoshida R, Ninomiya Y, Mikoshiba K
  • Journal Title: J. Biol. Chem. 21; 282(51) Pages: 37225-31
  • Peer Reviewed
• [Journal Article] Abnormal Taste Perception in Mice Lacking the Type 3 Inositol 1,4,5-Trisphosphate Receptor. (2007)
  • Author(s): Hisatsune C, Yasumatsu K, Takahashi-Iwanaga H, Ogawa N, Kuroda Y, Ybshida R, Ninomiya Y, Mikoshiba K.
  • Journal Title: J Biol Chem. 282 Pages: 37225-37231
  • Peer Reviewed
• [Presentation] Osteoblasts induce Ca^<2+> oscillation-independent NFATc1 activation during osteoclastogenesis (2008)
  • Author(s): Kuroda Yukiko
  • Organizer: Advanced Bone and Joint Science (ABJS) International Collaboration Symposium
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2008-12-04
• [Presentation] Osteoblasts induce Ca2+ oscillation-independent NFATc1 activation during osteoclastogenesis (2008)
  • Author(s): Kuroda Yukiko
  • Organizer: Advanced Bone and Joint Science (ABJS) International Collaboration Symposium
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2008-12-04