止血、癒着防止、抗菌効果を併せ持った新規ｉｎｊｅｃｔａｂｌｅゲルの開発
| Project/Area Number |
19F19061
|
|---|
| Research Category |
Grant-in-Aid for JSPS Fellows
|
| Allocation Type | Single-year Grants |
|---|
| Section | 外国 |
|---|
| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
伊藤 大知 東京大学, 大学院医学系研究科(医学部), 教授 (50447421)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
CHANDEL ARVIND 東京大学, 医学(系)研究科(研究院), 外国人特別研究員
|
|---|
| Project Period (FY) |
2019-04-25 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2019: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | hydrogels / antiadhesion barrier / dextran / PEG / hyaluronan / Anti-adhesion / Hemostatic / Injectable / Hydrogel / Biocompatible |
|---|
| Outline of Research at the Start |
The purpose of this study is to develop an antiadhesive, hemostatic, and antimicrobial smart polymeric material which overcomes the limits of available antiadhesive and haemostatic materials. Injectable hydrogel will be generated via block copolymer self-assembly, using copolymers of dextran and PDMS containing aldehyde and tertiary amine or acrylate. End-functionalized polyphosphate will be developed as in situ crosslinking agents. Gelation kinetics, biocompatibility, drug encapsulation/release property, and hemostatic/anti-adhesion property of the obtained material will be examined.
|
| Outline of Annual Research Achievements |
We have developed an injectable hydrogel via Dex-g-PDPMA-g-SRT crosslinked with Cl-PEG-Cl. Partially oxidized Dextran was grafted with Poly[2 (Dimethylamino)ethyl Methacrylate] (PDMAEMA) by ATRP to form Dex-g-PDMAEMA. Serotonin (SRT) was reacted with partially oxidized aldehyde containing Dex-g-PDMAEMA to form SRT-g-Dex-PDMAEMA, and chloride terminated poly(ethylene glycol) (Cl-PEG-Cl)was prepared 4-chloromethyl benzoyl chloride by the esterification reaction. The chemically crosslinked hydrogel was obtained, by the reaction between tertiary amine group of PDMAEMA and chloride terminated PEG. The gelation time of the hydrogel was 2-5 min its basically depends on concentration of the prepolymers solution and temperature. The In-vivo hemostatic behavior of hydrogel was evaluated using mice liver hemorrhage model. The hydrogel showed excellent hemostatic capacity in comparison to control. The hemostatic behavior of the hydrogel due to two component first formation of permanent positive charge on tertiary amine of PDMAEMA upon crosslinking and second one is pendent serotonin moiety. The hydrogel was compared with marketed hemostatic product TachoSil; and SURGICEL; using mice liver hemorrhage model. The hydrogel follows dual hemostatic mechanism, because it contains permanent cationic change that interact with red blood cells and serotonin moiety interact with platelet and accelerate the hemostasis synergistically.
|
| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
|
| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
|
Report
(2 results)
Research Products
(7 results)
