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止血、癒着防止、抗菌効果を併せ持った新規injectableゲルの開発

Research Project

Project/Area Number 19F19061
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionThe University of Tokyo

Principal Investigator

伊藤 大知  東京大学, 大学院医学系研究科(医学部), 教授 (50447421)

Co-Investigator(Kenkyū-buntansha) CHANDEL ARVIND  東京大学, 医学(系)研究科(研究院), 外国人特別研究員
Project Period (FY) 2019-04-25 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2019: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordshydrogels / antiadhesion barrier / dextran / PEG / hyaluronan / Anti-adhesion / Hemostatic / Injectable / Hydrogel / Biocompatible
Outline of Research at the Start

The purpose of this study is to develop an antiadhesive, hemostatic, and antimicrobial smart polymeric material which overcomes the limits of available antiadhesive and haemostatic materials. Injectable hydrogel will be generated via block copolymer self-assembly, using copolymers of dextran and PDMS containing aldehyde and tertiary amine or acrylate. End-functionalized polyphosphate will be developed as in situ crosslinking agents. Gelation kinetics, biocompatibility, drug encapsulation/release property, and hemostatic/anti-adhesion property of the obtained material will be examined.

Outline of Annual Research Achievements

We have developed an injectable hydrogel via Dex-g-PDPMA-g-SRT crosslinked with Cl-PEG-Cl. Partially oxidized Dextran was grafted with Poly[2 (Dimethylamino)ethyl Methacrylate] (PDMAEMA) by ATRP to form Dex-g-PDMAEMA. Serotonin (SRT) was reacted with partially oxidized aldehyde containing Dex-g-PDMAEMA to form SRT-g-Dex-PDMAEMA, and chloride terminated poly(ethylene glycol) (Cl-PEG-Cl)was prepared 4-chloromethyl benzoyl chloride by the esterification reaction. The chemically crosslinked hydrogel was obtained, by the reaction between tertiary amine group of PDMAEMA and chloride terminated PEG. The gelation time of the hydrogel was 2-5 min its basically depends on concentration of the prepolymers solution and temperature. The In-vivo hemostatic behavior of hydrogel was evaluated using mice liver hemorrhage model. The hydrogel showed excellent hemostatic capacity in comparison to control. The hemostatic behavior of the hydrogel due to two component first formation of permanent positive charge on tertiary amine of PDMAEMA upon crosslinking and second one is pendent serotonin moiety. The hydrogel was compared with marketed hemostatic product TachoSil; and SURGICEL; using mice liver hemorrhage model. The hydrogel follows dual hemostatic mechanism, because it contains permanent cationic change that interact with red blood cells and serotonin moiety interact with platelet and accelerate the hemostasis synergistically.

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (7 results)

All 2020 2019 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] In Situ Fabrication of Double-Layered Hydrogels via Spray Processes to Prevent Postoperative Peritoneal Adhesion2019

    • Author(s)
      Qi Pan、Zheng Ying Grace、Ohta Seiichi、Kokudo Norihiro、Hasegawa Kiyoshi、Ito Taichi
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: 5 Issue: 9 Pages: 4790-4798

    • DOI

      10.1021/acsbiomaterials.9b00791

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Zwitterionic hyaluronic acid-DMPA hydrogel for biomedical application2020

    • Author(s)
      Chandel Arvind Kumar Singh・Taichi Ito
    • Organizer
      11th World Biomaterials Congress
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Preparation of injectable hydrogel based on Dextran-g-PDMAEMA-g-Serotonin for hemostasis2020

    • Author(s)
      Chandel Arvind Kumar Singh・Ito Taichi
    • Organizer
      化学工学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Spray application of in situ cross-linkable powders for preventing esophageal stenosis after endoscopic submucosal dissection2019

    • Author(s)
      Pan Qi・Seiichi OHTA・Ayano FUJISAWA・Yosuke TSUJI・Mitsuhiro FUJISHIRO・Taichi ITO
    • Organizer
      Asian Pacific Confederation of Chemical Engineering 2019, Sapporo Convention Center, Japan, September 23-27, 2019, K414
    • Related Report
      2019 Annual Research Report
  • [Presentation] 癒着防止材の開発と応用展開2019

    • Author(s)
      伊藤大知
    • Organizer
      第46回日本血液浄化技術学会学術大会 お茶の水ソラシティ 東京 2019年4月20日
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Development of N, N dimethyl propyl amine conjugated hyaluronic acid zwitterionic hydrogel2019

    • Author(s)
      Chandel Arvind Kumar Singh・Ohta Seiichi・Ito Taichi
    • Organizer
      化学工学会 第85年会 関西大学千里山キャンパス 大阪 2020年3月15-17日 K307/li>
    • Related Report
      2019 Annual Research Report
  • [Remarks] 東京大学大学院医学系研究科 疾患生命工学センター 医療材料・機器工学部門 ホームページ

    • URL

      https://www.cdbim.m.u-tokyo.ac.jp/itolab/index.html

    • Related Report
      2020 Annual Research Report

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Published: 2019-05-29   Modified: 2024-03-26  

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