新規プローブライブラリーの構築に基づく大腸がん・胃がん検出蛍光プローブの開発
| Project/Area Number |
19F19339
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
浦野 泰照 東京大学, 大学院薬学系研究科(薬学部), 教授 (20292956)
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| Co-Investigator(Kenkyū-buntansha) |
KELLER SASCHA 東京大学, 薬学研究科(研究院), 外国人特別研究員
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| Project Period (FY) |
2019-11-08 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2021: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2019: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Fluorescence / Cancer / Tumor / Rhodol / Spiro-cyclic / Computer-based |
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| Outline of Research at the Start |
Goals of the project include: (i) development of a computer based model to predict properties of novel fluorescent molecules, (ii) synthesis and screening of a library of amino-acid and sugar Si-Rhodol-based probes that have promising properties; (iii) using those probes to visualize colon and stomach cancer as well as (iv) understanding of their overexpression of hydrolases in clinical specimens of cancerous colon and stomach tissue.
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| Outline of Annual Research Achievements |
This project aims at finding suitable probes to detect various cancers by rational design of fluorescence probes based on a quantum chemical prediction of their intramolecular spiro-cyclization. Goals of the project include: (i) development of a computer-based model to predict properties of novel fluorescent molecules, (ii) the synthesis of a library of amino-acid and sugar derivatives of novel Si- or C-Rhodols that have promising properties; (iii) using those probes to visualize colon and stomach cancer as well as (iv) understanding of their overexpression of aminopeptidases and glycosidases in clinical specimens of cancerous colon and stomach tissue. This fiscal year, the computational model for the prediction of the pKcycl-values of unknown Si-containing rhodols was extended to C-containing rhodols, and got satisfying results of pKcycl prediction, and compounds bound to sugar and amino acid moieties were synthesized by a significantly enhanced synthetic pathway. The 4ThP compound with 2 methyl groups showed well suited properties and was thus synthesized to be tested in cancerous specimens. Indeed, we could show that the probe bound to beta-galactose specifically turned fluorescent in breast cancer samples, while normal tissue almost did not show an increase in fluorescence. In conclusion, a versatile synthesis pathway and a calculation model was established.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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Report
(3 results)
Research Products
(3 results)
