| Project/Area Number |
19F19406
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
岡本 章玄 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, グループリーダー (70710325)
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| Co-Investigator(Kenkyū-buntansha) |
MIRAN WAHEED 国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, 外国人特別研究員
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| Project Period (FY) |
2019-11-08 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2021: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2020: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2019: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | Nitrogen fixing / Multi-heme Cytochrome C / Exciton coupling / Circular dichroism / バイオフィルム / 電気化学 / 二次イオン質量分析 / 電気細菌 / 膜小胞 |
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| Outline of Research at the Start |
細菌集団が作り出すバイオフィルムは、細菌による嫌気鉄腐食や感染症の原因であるが、物理的な除去以外の対策が未だ難しいという課題がある。本研究は、膜小胞（MV）と呼ばれる脂質二重層膜で作られた100ナノメートルサイズの生物学的ナノ粒子をバイオフィルム内へと薬剤を輸送するためのキャリアーとして用いる技術の確立へ向けた基礎的研究を行う。特に我々が見出したMVの持つ電気化学的特性がバイオフィルム内へ浸透する過程へ与える影響を生化学的・電気化学的に検討する。
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| Outline of Annual Research Achievements |
The electrochemical reactor systems currently used for current production and drug testing involve relatively large volumes, making these systems infeasible for high-throughput screening of potential antimicrobials. We used screen-printed electrodes or multi-well electrode systems that require minimum amounts of sample and antimicrobials. Such systems that exploit the electrogenic activity of pathogens could fast-track the discovery of antibiofilm drugs, as a large library of compounds could be rapidly screened. This concept could serve as a generally applicable technology for evaluating the efficacy of antimicrobials, as well as the selection of appropriate drugs or treatment regimens. Our works extended the boundaries of EET field to other niches such as human pathogens. The orthologues of the identified EET genes in human pathogens are present in hundreds of species, thus EET activity likely occurred in an evolutionarily diverse subset of bacteria. Consequently, our research can help screen the effects of antimicrobials on biofilm activity by employing the current producing capability in wide range of electroactive pathogens.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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