多様な生体医学応用のためのインテリジェント画像活性化細胞選抜法 の応用展開
| Project/Area Number |
19F19716
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 19010:Fluid engineering-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
合田 圭介 東京大学, 大学院理学系研究科(理学部), 教授 (70518696)
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| Co-Investigator(Kenkyū-buntansha) |
YUAN DAN 東京大学, 理学(系)研究科(研究院), 外国人特別研究員
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| Project Period (FY) |
2019-07-24 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2020: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2019: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | iIACS / imaging flow cytometry / cells / viscoelastic fluids / 3D focusing |
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| Outline of Research at the Start |
1) High performance sheathless viscoelastic focusing of biological cells three dimensionally (3D) based on the intrinsic viscoelastic fluid property.
2) Isolation of circulating tumor cells (CTCs) from blood for cancer basic research, clinical diagnosis and therapy based on IACS.
3) Detection and isolation activated platelets in fixed blood for accurate diagnosis of atherothrombosis and monitoring of anti-platelet therapy using IACS
4) Isolation of antigen-specific T cells from blood for immune study and therapy based on IACS.
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| Outline of Annual Research Achievements |
(a) The iIACS machine has been improved, which far surpasses the previous state-of-the-art iIACS machine in system performance, expanding the range of applications and discoveries. Its throughput and sensitivity are 20 times superior to those achieved in previous system.
(b) By employing the VIFFI microscope, the iIACS machine has higher imaging performance, holds promise for diverse applications in immunology, microbiology, stem cell biology, cancer biology, pathology, and synthetic biology. The imaging performance of the iIACS machine was validated using various cell types, like the FISH images of the Jurkat cells, imaging and sorting for the budding yeast cells.
(c) I have developed a viscoelastic fluid-based 3D focuser, and employed it for single-particle Raman analysis, as well as into the flow module in VIFFI platform, which can enable high-throughput single-cell imaging and analysis with higher stability.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(5 results)
