| Project/Area Number |
19F19718
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 46010:Neuroscience-general-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
風間 北斗 国立研究開発法人理化学研究所, 脳神経科学研究センター, チームリーダー (90546574)
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| Co-Investigator(Kenkyū-buntansha) |
KIRSZENBLAT LEONIE 国立研究開発法人理化学研究所, 脳神経科学研究センター, 外国人特別研究員
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| Project Period (FY) |
2019-07-24 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2019: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | sleep / olfaction / calcium imaging / optogenetics / Drosophila |
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| Outline of Research at the Start |
Sleep is a state of quiescence during which an animal loses awareness of the external world. In this study, we will investigate the neural mechanisms by which the brain suppresses perception during sleep, with a focus on the Drosophila olfactory system.
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| Outline of Annual Research Achievements |
We have made efforts toward building a set up in which olfactory circuits can be imaged under the microscope while simultaneously monitoring the fly’s behavior and detecting when it is asleep. We have also engineered a fly strain in which we can simultaneously induce sleep and monitor neural activity. The behavior of these flies was examined and it was found that upon exposure to 617nm red-light, the flies become quiescent, and when the light is switched off they become active again. These experiments confirm that this optogenetic tool can be used as a ‘sleep switch’ to transiently induce sleep by activating specific neurons in the fly brain. We then examined the activity of these neurons using calcium imaging, and confirmed that neural activity is increased during red light exposure. In parallel to testing sleep, we have also begun to record brain responses to different odors, allowing us to optimize our imaging settings for future sleep and sleep deprivation experiments.
We expect our study will provide fundamental insights into how sleep affects sensory processing. Since a number of studies have shown that sleep in the fruit fly, Drosophila, shares many similarities with sleep in humans and other animals, any of the neural mechanisms and functions of sleep that we uncover here will likely be conserved in other animals.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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