Towards integrating the AHL transcription factors into a plant growth gene regulatory network
| Project/Area Number |
19F19781
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
杉本 慶子 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (30455349)
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| Co-Investigator(Kenkyū-buntansha) |
FAVERO DAVID 国立研究開発法人理化学研究所, 環境資源科学研究センター, 外国人特別研究員
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| Project Period (FY) |
2019-11-08 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2021: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2019: ¥100,000 (Direct Cost: ¥100,000)
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| Keywords | AHL / SOB3 / petiole / PIF / transcription / growth / plants / photomorphogenesis |
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| Outline of Research at the Start |
The aim of this project is to establish where AT-HOOK MOTIF NUCLEAR LOCALIZED(AHL) transcription factors(TFs) fit withing gene regulatory networks(GRNs) which modulate growth in response to environmental stimuli in the model Plant Arabidopsis thaliana.
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| Outline of Annual Research Achievements |
My work during the past funding period has provided new insights into the mechanisms by which the AHL transcription factors regulate transcription and growth in Arabidopsis. Together with my collaborators at Washington State University, I finished generating data for and writing a manuscript that describes how AHLs regulate gene expression and suppress hypocotyl growth in seedlings grown in short days. We recently resubmitted this manuscript to BMC Plant Biology, where it has been provisionally accepted. In this manuscript, we describe how AHLs affect the expression of different sets of genes at different times throughout the course of a short day. For example, we showed that SOB3 and/or other AHLs repress the expression of the PIF-activated, growth-promoting genes XTR7 and CDF5 at ZT9 and ZT24, but not at ZT4. On the other hand, we found that SOB3 and/or other AHLs activate the expression of the growth-repressing genes HY5, BBX22, RGA, and PRR9 specifically at ZT4. However, we unexpectedly also found that SOB3 exhibits similar patterns of binding to target genes at ZT4, ZT9, and ZT24. Thus, our data from this study collectively suggest that AHLs affects gene expression in a time-point specific manner irrespective of changes in binding to DNA throughout short days. Apart from work related to the BMC Plant Biology manuscript, I have also recently generated data suggesting that AHLs affect PIF activity at the protein level, i.e. they can reduce PIF-mediated activation of target genes without affecting PIF protein levels.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Trihelix transcription factors GTL1 and DF1 prevent aberrant root hair formation in an excess nutrient condition.2022
Author(s)
Shibata, M., Favero, D.S., Takebayashi, R., Takebayashi, A, Kawamura, A, Rymen, Hosokawa, Y, and Sugimoto, K.
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Journal Title
New Phytologist
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Wound-inducible WUSCHEL-RELATED HOMEOBOX 13 is required for callus growth and organ reconnection2021
Author(s)
Ikeuchi Momoko, Iwase Akira, Ito Tasuku, Tanaka Hayato, Favero David S, Kawamura Ayako, Sakamoto Shingo, Wakazaki Mayumi, Tameshige Toshiaki, Fujii Haruki, Hashimoto Naoki, Suzuki Takamasa, Hotta Kazuhiro, Toyooka Kiminori, Mitsuda Nobutaka, Sugimoto Keiko
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Journal Title
Plant Physiology
Volume: 188
Issue: 1
Pages: 425-441
DOI
Related Report
Peer Reviewed / Open Access
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