| Project/Area Number |
19F19782
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
白須 賢 国立研究開発法人理化学研究所, 環境資源科学研究センター, グループディレクター (20425630)
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| Co-Investigator(Kenkyū-buntansha) |
FISHMAN MAX 国立研究開発法人理化学研究所, 環境資源科学研究センター, 外国人特別研究員
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| Project Period (FY) |
2019-11-08 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2021: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2019: ¥400,000 (Direct Cost: ¥400,000)
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| Keywords | Parasiticplants / Calciumsignaling / phosphoproteomics / DMBQ / hustorium / parasiticplants / calciumsignaling / haustorium / parasitic plants / calcium signaling |
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| Outline of Research at the Start |
The molecular biology and signaling pathway that initiates haustorium induction in parasitic flowering plants is not well characterized. Haustorium induction can be initiated in Phtheirospermum japonicum with the haustorium inducing factor dimethoxy benzoquinone (DMBQ). I plan to use P. japonicum in order to identify primary signaling components responsible for haustorium induction following DMBQ treatment. My goal is to identify receptor-like kinases (RLKs), co-receptors, or transcription factors found within the primary signaling pathway responsible for DMBQ-induced haustorium development.
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| Outline of Annual Research Achievements |
During 2021, I identified endogenous Phtheirospermum japonicum peptide hormones that are induced upon treatment with the haustorium inducing compound (HIF) 2,6 dimethoxybenzoquinone (DMBQ). I found that treatment of P. japonicum with an endogenous peptide induced haustorium formation without the treatment of an HIFs and induced expression of the known haustorium formation marker gene PjYUC3. Further investigation revealed that there are three additional putative peptide hormones in the P. japonicum genome and six putative peptide receptors. Further investigation found that only one of those three peptides is up-regulated following DMBQ treatment while none of the receptors are up-regulated following DMBQ treatment. I further characterized the expression of the peptides and receptors using transformed P. japonicum hairy roots expressing reporter gene constructs. I found that only one of the four peptides and potentially three of the receptors are expressed in the haustorium following DMBQ treatment. Lastly, PLETHORA (PLT) transcription factors are stabilized post-translationally by the peptides. Using hairy root transformation, I found that DMBQ treatment resulted in movement of a PjPLT protein up the root and that this PjPLT localized in the haustorium.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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