Single Molecule Mechanics and Computer Modeling of Cascading System of Biological Information Transfer

• Project/Area Number: 19GS0418
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: IKAI Atsushi 東京工業大学, イノベーション研究推進体, 特任教授 (50011713)
• Co-Investigator(Kenkyū-buntansha): KISHIMOTO Kikuo 東京工業大学, 大学院・理工学研究科, 教授 (30111652) ; OSADA Toshiya 東京工業大学, 大学院・生命理工学研究科, 准教授 (00201997) ; OHTANI Hiroyuki 東京工業大学, 大学院・生命理工学研究科, 准教授 (80203826) ; AFRIN Rehana 東京工業大学, バイオフロンティアセンター, 特任准教授 (70447556) ; TAGAMI Katsunor 早稲田大学, 特任講師 (40361571) ; SEKIGUCHI Hiroshi 東京工業大学, 大学院・生命理工学研究科, 助教 (00401563)
• Project Period (FY): 2007 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥468,390,000 (Direct Cost: ¥360,300,000、Indirect Cost: ¥108,090,000); Fiscal Year 2011: ¥74,490,000 (Direct Cost: ¥57,300,000、Indirect Cost: ¥17,190,000); Fiscal Year 2010: ¥73,320,000 (Direct Cost: ¥56,400,000、Indirect Cost: ¥16,920,000); Fiscal Year 2009: ¥80,340,000 (Direct Cost: ¥61,800,000、Indirect Cost: ¥18,540,000); Fiscal Year 2008: ¥105,300,000 (Direct Cost: ¥81,000,000、Indirect Cost: ¥24,300,000); Fiscal Year 2007: ¥134,940,000 (Direct Cost: ¥103,800,000、Indirect Cost: ¥31,140,000)
• Keywords: 原子間力顕微鏡 / 細胞骨格 / ネットワーク力学 / 分子間相互作用力 / ストレスファイバー / アクチン / 生体力学情報伝達 / 生体ナノ力学 / リンカータンパク質 / パキシリン / 有限要素法 / 分子動力学 / 生体情報伝達 / 膜タンパク質 / ナノ力学 / アクチン繊維 / 原子間力顕微鏡(AFM) / 力学的分光法 / 力学的信号伝達系 / ナノバイオメカニクス

Research Abstract

The aim of this project is to perform mechanically quantitative experiments to clarify the mechanical and wound healing characteristics of the cytoskeletal and contribute to the single cell manipulations and surgery. We used a newly developed nano-mechanical methods to measure the different mechanical properties of the red blood cell and fibroblast(especially those of actin stress fibers) and constructed mechanical models to simulate the results. We obtained solid mechanical evidence that showed strong association of stress fibers with cortical actomyosin layer. This association forms the basis for the cytoskeleton and cell membrane cooperation during cell locomotion and information transfer.

Research Products

• [Journal Article] Forced Extension of delipidated red blood cell cytoskeleton with little indication of spectrin unfolding (2012)
  • Author(s): Afrin R, Nakaji M, Sekiguchi H, Lee D, Kishimoto K, Ikai A
  • Journal Title: Cytoskeleton Volume: 69巻 Issue: 2 Pages: 101-112
  • DOI: 10.1002/cm.21001
  • Peer Reviewed
• [Journal Article] Fabricated cantilever for AFM measurements and manipulations (2012)
  • Author(s): Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu Saito, Rehana Afrin, and Atsushi Ikai
  • Journal Title: Pre-stress analysis of stress fibers, Micron Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Interaction of Serum Proteins with Surface of Hemodialysis Fiber Membranes (2012)
  • Author(s): Rehana Afrin, Yuji Shirako, Kikuo Kishimoto, Atsushi Ikai
  • Journal Title: Japanese Journal of Applied Physics Volume: (印刷中)
  • NAID: 210000141141
  • Peer Reviewed
• [Journal Article] Fabricated cantilever for AFM measurements and manipulations : Pre-stress analysis of stress fibers (2012)
  • Author(s): Shinich Machida, Takahiro Watanabe-Nakayama, Masakazu Saito, Rehana Afrin, Atsushi Ikai
  • Journal Title: Micron Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Nonlinear displacement of ventral stress fibers under externally applied lateral force by an atomic force microscope (2011)
  • Author(s): Hakari T, Sekiguchi H, Osada T, Kishimoto K, Afrin R, Ikai A
  • Journal Title: Cytoskeleton Volume: 68巻 Issue: 11 Pages: 628-638
  • DOI: 10.1002/cm.20537
  • Peer Reviewed
• [Journal Article] Direct detection of cellular adaptation to local cyclic stretching at the single cell level by atomic force microscopy (2011)
  • Author(s): Watanabe-Nakayama T, Machida S, Harada I, Sekiguchi H, Afrin R, Ikai A
  • Journal Title: Biophysical Journal Volume: 100巻 Pages: 564-572
  • Peer Reviewed
• [Journal Article] Micro-scoop for Manipulation of Micro Objects (2010)
  • Author(s): Takahiro Watanabe-Nakayama, Shinichi Machida, Rehana Afrin, and Atsushi Ikai
  • Journal Title: Use of Fabricated Cantilever with Atomic Force Microscope、Small Volume: 6巻 Pages: 2853-2857
  • Peer Reviewed
• [Journal Article] Direct manipulation of intracellular stress fibres using a hook-shaped AFM probe (2010)
  • Author(s): Shinichi Machida, Takahiro Watanabe-Nakayama, Ichiro Harada, Rehana Afrin, Tomonobu Nakayama and Atsushi Ikai
  • Journal Title: Nanotechnology Volume: 21巻
  • Peer Reviewed
• [Journal Article] Tensile Mechanics of α-helical coil springs, Adv (2010)
  • Author(s): Atsushi Ikai
  • Journal Title: Polymer Sci. (印刷中)
  • Peer Reviewed
• [Journal Article] Tensile Mechanics of Alanine Based Helical Polypeptide (2009)
  • Author(s): Rehana Afrin, Kazuki Shiga, Ichiro Takahashi and Atsushi Ikai
  • Journal Title: Force Spectroscopy vs. Computer Simulations, Biophys. J Volume: 96巻 Pages: 1105-1114
  • Peer Reviewed
• [Journal Article] Atomic force microscopy for cellular level manipulation: imaging intracellular structures and DNA delivery through a membrane hole. (2009)
  • Author(s): Afrin R, Zohora US, Uehara H, Watanabe-Nakayama T, Ikai A.
  • Journal Title: J Mol Recognit 22 Pages: 363-372
  • Peer Reviewed
• [Journal Article] Single molecular dynamic interactions between glycophorin A and lectin as probed by atomic force microscopy (2009)
  • Author(s): Yan C, Yersin A, Afrin R, Sekiguchi H, Ikai A.
  • Journal Title: Biophys Chem 144 Pages: 72-77
  • Peer Reviewed
• [Journal Article] Tensile mechanics of alanine-based helical polypeptide: force spectroscopy versus computer simulations. (2009)
  • Author(s): Afrin R, Takahashi I, Shiga K, Ikai A
  • Journal Title: Biophys J 96 Pages: 1105-1114
  • Peer Reviewed
• [Journal Article] Tensile Mechanics of Alanine Based Helical Polypeptide: Force Spectroscopy vs. Computer Simulations (2009)
  • Author(s): Rehana Afrin
  • Journal Title: Biophys. J. 96 Pages: 1105-1114
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 猪飼篤
  • Journal Title: 走査型プローブ顕微鏡(共立出版) Pages: 123-131
• [Journal Article] Exploring Transferrin-Receptor Interactions at the Single-Molecule Level (2008)
  • Author(s): Alexandre Yersin、Toshiya Osada, Atsushi Ikai
  • Journal Title: Biophys. J Volume: 94巻 Pages: 230-240
  • Peer Reviewed
• [Journal Article] Exploring transferrin-receptor interactions at the single-molecule level (2008)
  • Author(s): Yersin A, Osada T, Ikai A.
  • Journal Title: Biophys J 94 Pages: 230-240
  • Peer Reviewed
• [Journal Article] Nanobiomechanics of proteins and biomembrane (2008)
  • Author(s): Atsushi Ikai
  • Journal Title: Phil. Trans. R. Soc. B 363 Pages: 2163-2171
  • Peer Reviewed
• [Journal Article] Exploring Transferrin-Receptor Interactions at the Single-Molecule Level (2008)
  • Author(s): Alexandre Yersin
  • Journal Title: Biophys. J. 94 Pages: 230-240
  • Peer Reviewed
• [Journal Article] Distribution of olfactory marker protein on a tissue section of vomeronasal organ measured by AFM (2008)
  • Author(s): Hyonchol Kim
  • Journal Title: Colloids and Surfaces B: Biointerfaces 61 Pages: 311-314
  • Peer Reviewed
• [Journal Article] Exploling transferrin-receptor interactions at the single-molecule level (2008)
  • Author(s): Yersin, A., Osada, T., Ikai A.
  • Journal Title: Biophysical Journal 94 Pages: 230-240
  • Peer Reviewed
• [Journal Article] Distribution of Olfactory Marker Protein on a Tissue Section of Vomeronasal Organ Measured by AFM (2008)
  • Author(s): Kim H, Asgari F, Osada T, Ichikawa M, Ikai A.
  • Journal Title: Colloids and Surfaces B 61
  • Peer Reviewed
• [Journal Article] Nano-Biomechanics of Proteins and Biomembrane (2008)
  • Author(s): Atsushi Ikai
  • Journal Title: Phylosophical Transaction of the Royal Society (インターネット版)
  • Peer Reviewed
• [Journal Article] Pulling and Pushing Protein Molecules by AFM (2007)
  • Author(s): Ikai, A., Afrin R., and H. Sekiguchi
  • Journal Title: Current Nanoscience 3 Pages: 17-29
  • Peer Reviewed
• [Journal Article] Granular tau oligomers as intermediates of tau filaments (2007)
  • Author(s): Maeda S, Sahara N, Saito Y, Murayama M, Yoshiike Y, Kim H, Miyasaka T, Murayama S, Ikai A, Takashima A.
  • Journal Title: Biochemistry 46 Pages: 3856-3861
  • Peer Reviewed
• [Journal Article] mRNA detection of individual cells with the single cell nanoprobe method compared with in situ hybridization (2007)
  • Author(s): Uehara, H., Kunitomi, Y., Ikai A., and Osada, T.
  • Journal Title: Journal of Nanobiotechnology 5 Pages: 7-12
  • Peer Reviewed
• [Journal Article] 界面き裂の支配力学パラメータと界面強度評価試験 (2007)
  • Author(s): 岸本喜久雄, 大宮正毅, 上月謙太郎
  • Journal Title: 日本接着学会誌 43 Pages: 31-39
  • NAID: 10021159046
  • Peer Reviewed
• [Journal Article] 粗視化分子動力学を用いた高分子系接着剤のはく離解析 (2007)
  • Author(s): 大宮正毅, 篠崎明, 井上裕嗣, 岸本喜久雄
  • Journal Title: 日本機械学会第20回計算力学講演会論文集 Pages: 67-68
• [Journal Article] 破壊力学をプローブとして見るタンパク質内部構造 (2007)
  • Author(s): 猪飼 篤
  • Journal Title: 生物物理 47 Pages: 241-248
  • NAID: 110006369068
  • Peer Reviewed
• [Journal Article] ナノ領域の力学的摂動分光 (2007)
  • Author(s): 児玉 高志, 荒川 秀雄, 猪飼 篤, 大谷 弘之
  • Journal Title: 生物物理, 47 Pages: 44-48
  • NAID: 110006201588
  • Peer Reviewed
• [Presentation] Paxillin accumulation to a damaged site on a stress fiber (2012)
  • Author(s): Takahiro Watanabe-Nakayama, Masakazu Saito, Shin-ichi Machida, Rehana Afrin, and Atsushi Ikai
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Exploring Atomic Force Microscopy for Nano-medical Applications (2012)
  • Author(s): Rehana Afrin, Takahiro Watanabe-Nakayama, Shinichi Machida, Masakazu, Saito and Atsushi Ikai
  • Organizer: ACMM22/ICONN2012/APMC10 Combined Conference
  • Place of Presentation: Perth, Australia
• [Presentation] AFM for Cytoskeletal Mechanics (2012)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito and Kikuo Kishimoto
  • Organizer: Linz Winter Workshop 2012
  • Place of Presentation: Lintz, Austria
• [Presentation] AFM for Cytoskeletal Mechanics (2012)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito, Kikuo Kishimoto
  • Organizer: Linz Winter Workshop 2012
  • Place of Presentation: Lintz, Austria(invited)
• [Presentation] Paxillin accumulation to a damaged site on a stress fiber (2012)
  • Author(s): Takahiro Watanabe-Nakayama, Masakazu Saito, Shin-ichi Machida, Rehana Afrin, Atsushi Ikai
  • Organizer: Biophysical Society 56^<th> Annual Meeting
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Single molecule dynamic interactions between spectrin and ankyrin studied by atomic force microscopy (2012)
  • Author(s): Masakazu Saito, Takahiro W.Nakayama, Shinichi Machida, Rehana Afrin, Atsushi Ikai
  • Organizer: XIVth Annual Linz Winter Workshop
  • Place of Presentation: Linz, Austria
• [Presentation] マニピュレーション機能をもつカンチレバーの開発と1細胞研究への適用 (2011)
  • Author(s): 中山隆宏
  • Organizer: 東京大学生産技術研究所・JPK Instruments AG共同開催ワークショップ「生命科学における走査プローブ法と光ピンセット」
  • Place of Presentation: Tokyo, Japan(invited)
  • Year and Date: 2011-09-28
• [Presentation] Mechanical Analysis of the Bio Cell Subjected to AFM Loading (2011)
  • Author(s): Kikuo Kishimoto, David Lee, Yotaro Shinosawa, Kazuaki Inaba, Rehana Afrin and Atsushi Ikai
  • Organizer: International Workshop on Biomedical Engineering & Biomechanics(invited)
  • Place of Presentation: 西安,中国
  • Year and Date: 2011-08-13
• [Presentation] Mechanical Analysis of the Bio Cell Subjected to AFM Loading (2011)
  • Author(s): Kikuo Kishimoto, David Lee, Yotaro Shinosawa, Kazuaki Inaba, Rehana Afrin, Atsushi Ikai
  • Organizer: International Workshop on Biomedical Engineering & Biomechanics
  • Place of Presentation: 西安,中国(invited)
  • Year and Date: 2011-08-13
• [Presentation] Integrity Assessment of Multi-Material Structures and Smart Structure Technology (2011)
  • Author(s): Kikuo Kishimoto
  • Organizer: ASPIRE Symposium
  • Place of Presentation: KAIST,韓国
  • Year and Date: 2011-07-07
• [Presentation] Linkage structures between ventral stress fibers revealed by atomic force and fluorescence microscopy、Seeing is Believing (2011)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito and Kikuo Kishimoto
  • Organizer: Imaging the Processes of Life
  • Place of Presentation: Santa Barbara, USA
• [Presentation] Manipulation of Individual Stress Fibers and Focal Adhesions with AFM to Understand the Basic Mechanics of the Cellular (2011)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito and Kikuo Kishimoto
  • Organizer: Construction, AFM for Bio Med, Paris
  • Place of Presentation: Paris, France
• [Presentation] Evaluation of the Interfacial Strength of Mutilyaered Materials by Peeling & Indentation Method (2011)
  • Author(s): Kikuo Kishimoto
  • Organizer: AUN/SEED-Net 3rd Regional Conference in Mechanical and Aerospace Technology
  • Place of Presentation: Manila, Phillipines
• [Presentation] Concerted Displacement of Stress Fibers in vivo under Direct Mechanical Manipulation (2011)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito, Kikuo Kishimoto
  • Organizer: BIT 1st Annual World Congress of Molecular & Cell Biology (CMCB-2011)
  • Place of Presentation: Beijing, China(invited)
• [Presentation] Biomechanics of Cytoskeletal Structures (2011)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito, Kikuo Kishimoto
  • Organizer: the Eleventh AEARU Workshop on Molecular Biology and Biotechnology
  • Place of Presentation: Hong Kong, China(invited)
• [Presentation] Manipulation of Individual Stress Fibers and Focal Adhesions with AFM to Understand the Basic Mechanics of the Cellular Construction (2011)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito, Kikuo Kishimoto
  • Organizer: AFM for BioMed Paris 2011
  • Place of Presentation: Paris, France
• [Presentation] Lateral Displacement of Stress Fibers under Direct Mechanical Manipulation (2011)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Masakazu Saito, Takahiro Nakayama-Watanabe
  • Organizer: International Conference on Surface Sciences (ISSS) 6
  • Place of Presentation: Funabori, Japan
• [Presentation] Linkage structures between ventral stress fibers revealed by atomic force and fluorescence microscopy (2011)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shinichi Machida, Takahiro Watanabe-Nakayama, Masakazu, Saito, Kikuo Kishimoto
  • Organizer: Seeing is Believing : Imaging the Processes of Life
  • Place of Presentation: Santa Barbara, USA
• [Presentation] Exploring Atomic Force Microscopy for Single Cell Manipulations (2011)
  • Author(s): Rehana Afrin, Takahiro Watanabe-Nakayama, Shinichi Machida, Masakazu, Saito, Atsushi Ikai
  • Organizer: Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: Denver, CO, USA
• [Presentation] Interaction Between Serum Proteins And Polymer Surface Studied By Atomic Force Microscopy (2011)
  • Author(s): Rehaha Afrin, Takahiro Watanabe-Nakayama, Shinichi Machida, Masakazu, Saito, Atsushi Ikai
  • Organizer: AFM-BioMed Conference
  • Place of Presentation: Paris, France
• [Presentation] Mechanical Interactions of Red Blood Cell Cytoskeleton and Associated Proteins (2011)
  • Author(s): Rehana Afrin, Takahiro Watanabe-Nakayama, Shinichi Machida, Masakazu, Saito, Atsushi Ikai
  • Organizer: 13th International Scanning Probe Microscopy Conference (ISPM)
  • Place of Presentation: Munich, Germany
• [Presentation] Aomic Force Microscopy for Nano-medical Applications (2011)
  • Author(s): Rehana Afrin, Takahiro Watanabe-Nakayama, Shinichi Machida, Masakazu, Saito, Atsushi Ikai
  • Organizer: 2nd International NanoMedicine Conference
  • Place of Presentation: Sydney, Australia
• [Presentation] Interaction of Serum Proteins with the Surface of Hemodialysis Tubing (2011)
  • Author(s): Rehana Afrin, Takahiro Watanabe-Nakayama, Shinichi Machida, Masakazu, Saito, Atsushi Ikai
  • Organizer: International Colloquium of Scanning Probe Microscopy (ICSPM19)
  • Place of Presentation: Toyako, Japan
• [Presentation] Application of Micro Scoop Cantilever for Cell Biophysics (2011)
  • Author(s): Takahiro Watanabe-Nakayama, Shin-ichi Machida, Masakazu Saito, Rehana Afrin, Atsushi Ikai
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: Himeji, Japan
• [Presentation] Paxillin accumulation at strained stress fibers (2011)
  • Author(s): Takahiro Watanabe-Nakayama, Masakazu Saito, Shin-ichi Machida, Rehana Afrin, Atsushi Ikai
  • Organizer: 日本分子生物学会第34回年会
  • Place of Presentation: Yokohama, Japan
• [Presentation] Single molecular dynamic interactions between spectrin and ankyrin studied by atomic force microscopy (2011)
  • Author(s): 齋藤勝和、中山隆宏、町田真一、レハナアフリン、猪飼篤
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] Paxillin accumulation at strained stress fibers (2011)
  • Author(s): 中山(渡部)隆宏、齋藤勝和、町田真一、Rehana Afrin、猪飼篤
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] 原子間力顕微鏡を用いたアクチンフィラメントとビンキュリンテイルの相互作用力測定 (2011)
  • Author(s): 笠原慧、中山隆宏、町田真一、斉藤勝和、長田俊哉、レハナアフリン、猪飼篤
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
• [Presentation] フェロモン受容体の分裂酵母細胞膜上での発現効率の研究 (2011)
  • Author(s): 金高友貴恵, 猪飼篤, 長田俊哉
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 分裂酵母を用いた哺乳類GPCRの機能的発現の試み (2011)
  • Author(s): 清崎彰一, 猪飼篤, 長田俊哉
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Direct Manipulation of Stress Fibers in the Cell Using Fabricated Atomic Force Microscopy Cantilevers、American Biophysical Society Symposium (2010)
  • Author(s): Shinichi Machida, Takahiro W. Nakayama, Ichiro Harada, Rehana Afrin, Tomonobu Nakayama, Atsushi Ikai
  • Organizer: Actin, the Cytoskeleton and the Nucleus
  • Place of Presentation: Singapore
  • Year and Date: 2010-11-09
• [Presentation] Force Measurement and Intracellular Operation Using Customized AFM Cantilever、American (2010)
  • Author(s): S. Machida, T. W. Nakayama ; I. Harada ; R. Afrin ; T. Nakayama ; A. Ikai
  • Organizer: Biophysical Society 54th Annual Meeting
  • Place of Presentation: San Francisco USA
• [Presentation] Force Spectroscopy of Helical Polypeptides and Cytoskeleton Related Proteins Studied by Atomic Force Microscopy (2010)
  • Author(s): Atsushi Ikai, Rehana Afrin, Shin-ichi Machida and Takahiro Nakayama, David Lee and Kikuo Kishimoto
  • Organizer: The 17th International Microscopy Congress(IMC17)
  • Place of Presentation: Rio de Janeiro Brazil
• [Presentation] Direct Detection OF Tension Recovery After Local Stretching of Cell Surface (2010)
  • Author(s): Takahiro W. Nakayama, Shin-ichi Machida, Ichiro Harada, Hiroshi Sekiguchi, Rehana Afrin, Atsushi Ikai
  • Organizer: American Biophysical Society 54th Annual Meeting
  • Place of Presentation: San Francisco, U.S.A.
• [Presentation] 原子間力顕微鏡を用いたヒト赤血球細胞骨格の力学的測定 (2009)
  • Author(s): 町田真一
  • Organizer: 第56回応用物理学関係連合講演会
  • Place of Presentation: つくば市
  • Year and Date: 2009-03-31
• [Presentation] "Local mechanics of cytoskeletal networks probed by AFM" WBMA'09 Watching Biomolecules in Action (2009)
  • Author(s): Atsushi Ikai
  • Organizer: Single Molecule Biology Symposium
  • Place of Presentation: Osaka, Japan
• [Presentation] Single Molecule Force Spectroscopy between Erythrocyte Glycoproteins and Sugar Specific Lectins (2009)
  • Author(s): Rehana Afrin, Atsushi Ikai
  • Organizer: Watching Biomolecules in Actions (WBMA '09), Single Molecule Biology Symposium
  • Place of Presentation: Osaka
• [Presentation] Monte Carlo Simulation Study of a Red Blood Cell Cytoskeleton Under AFM Loading (2009)
  • Author(s): D Lee, K Kishimoto, A Ikai, K Inaba
  • Organizer: Asia Pacific Conference on Mechanics of Materials
  • Place of Presentation: Yokohama, JAPAN
• [Presentation] Pulling Proteins From the Cell Surface with AFM (2008)
  • Author(s): Atsushi Ikai
  • Organizer: JPK 7th annual symposium on SPM for the Life Sciences
  • Place of Presentation: Berlin Germany
  • Year and Date: 2008-12-28
• [Presentation] AFMによる生体分子間相互作用力測定 (2008)
  • Author(s): 関口博史
  • Organizer: 財団法人 新世代研究所 バイオSPM研究会
  • Place of Presentation: 新潟 日本
  • Year and Date: 2008-03-14
• [Presentation] 溶液中環境下でのAFMによるタンパク質力学計測 (2008)
  • Author(s): 関口博史
  • Organizer: 日本顕微鏡学会 走査型プローブ顕微鏡分科会 平成19年度研究会
  • Place of Presentation: 京都 日本
  • Year and Date: 2008-03-07
• [Presentation] Nano-indentation of Single Protein Investigated by Atomic Force Microscopy (2008)
  • Author(s): H. Sekiguchi, Y. Mori, A. Ikai
  • Organizer: Protein Research International Symposium on "New Approaches to Complexity of Protein Dynamics by Single Molecule Measurements: Experiments and Theories"
  • Place of Presentation: Icho-Kaikai, Osaka univ
• [Presentation] High sensitive detection of proteins using gel electrophoresis and atomic force microscopy (2008)
  • Author(s): Hiroshi Sekiguchi
  • Organizer: 10th International Scanning Probe Microscopy Conference
  • Place of Presentation: Seattle米国
• [Presentation] Nano-indentation of Single Protein Investigated by Atomic Force Microscopy (2008)
  • Author(s): Hiroshi Sekiguchi
  • Organizer: Protein Research International Symposium on "New Approaches to Complexity of Protein Dynamics by Single Molecule Measurements: Experiments and Theories
  • Place of Presentation: 大阪
• [Presentation] AFMによるタンパク質分子の圧縮:実験とシミュレーション (2008)
  • Author(s): 関口博史
  • Organizer: 日本顕微鏡学会第64回学術講演会
  • Place of Presentation: 京都
• [Presentation] AFM力学測定の生命科学への応用:精度良い測定に向けて (2008)
  • Author(s): 関口博史
  • Organizer: 第28回表面科学学術講演会
  • Place of Presentation: 東京
• [Presentation] Applications of AFM Force Measurement for Life-science Field (2008)
  • Author(s): Hiroshi Sekiguchi
  • Organizer: 日本生物物理学会第46会年会
  • Place of Presentation: 福岡
• [Presentation] Subunit Unbinding Mechanics of a Dimeric Protein Studied By Atomic force Microscopy (2008)
  • Author(s): Rehana Afrin
  • Organizer: International Conference on Nanoscience and Technology
  • Place of Presentation: Denver米国
• [Presentation] Subunit Unbinding Mechanics of a Dimeric Protein Studied By Atomic force Microscopy (2008)
  • Author(s): Rehana Afrin
  • Organizer: JPK 7th annual symposium on SPM for the Life Sciences
  • Place of Presentation: Berlinドイツ
• [Presentation] Cellular Level Manipulation by Atomic Force Microscopy (2008)
  • Author(s): Rehana Afrin
  • Organizer: International Meeting on AFM in Life Sciences and Medicine (AFM BioMed 2008)
  • Place of Presentation: Monterey米国
• [Presentation] Lateral force measurement by AFM and applications in cell biology (2008)
  • Author(s): Tomoro Hakari
  • Organizer: JPK 7th annual symposium on SPM for the Life Sciences
  • Place of Presentation: Berlinドイツ
• [Presentation] 細胞接着に付随する細胞骨格の再構築に関するAFMによる力学解析 (2008)
  • Author(s): 中山隆宏
  • Organizer: 第28回表面科学学術講演会
  • Place of Presentation: 東京
• [Presentation] 細胞-細胞外マトリックス結合力のAFMを用いた解析 (2008)
  • Author(s): 中山隆宏
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡
• [Presentation] Unbinding Mechanics of Specific Lectins from Their Target Proteins on the Cell Surface (2008)
  • Author(s): Rehana Afrin, Masato Nakaji and Atsushi Ikai.
  • Organizer: The Xth Linz Annual Winter Workshop 2008
  • Place of Presentation: Linz, Austria.
• [Presentation] Interaction between transferrin and transferrin receptor investigated by atomic force microscopy (2008)
  • Author(s): Yersin A., Katsuya Ikuta and Ikai A.
  • Organizer: The Xth Linz Annual Winter Workshop 2008
  • Place of Presentation: Linz, Austria.
• [Presentation] タンパク質の表面吸着と破壊 (2008)
  • Author(s): 関口博史
  • Organizer: 日本表面科学会第3回ソフトナノテクノロジー研究部会
  • Place of Presentation: 東京 日本
• [Presentation] Transferrin-Receptor Interaction Investigated by AFM (2008)
  • Author(s): A. Yersin
  • Organizer: 日本表面科学会第3回ソフトナノテクノロジー研究部会
  • Place of Presentation: 東京 日本
• [Presentation] タンパク質分子力学の実験と分子動力学シミュレーション (2008)
  • Author(s): 関口博史, 田上勝規, 森 泰啓, 塚田捷, 猪飼 篤
  • Organizer: ナノプローブテクノロジー第167委員会 第49回研究会
  • Place of Presentation: 東京 日本
• [Presentation] Nano-Biomechanics (2007)
  • Author(s): Atsushi Ikai
  • Organizer: インドネシア化学会
  • Place of Presentation: Bandung, Indonasia
  • Year and Date: 2007-07-13
• [Presentation] Exploring Promising Applications of Atomic Force Microscope as a Bionano-technological Tool (2007)
  • Author(s): Rehana Afrin, Atsushi Ikai
  • Organizer: International Scanning Probe Microscopy Conference (ISPM)
  • Place of Presentation: Jeju, SOUTH KOREA
• [Presentation] Exploring Promising Applications of Atomic Force Microscope as a Bionanotechnological Tool. (2007)
  • Author(s): Rehana Afrin, Hironori Uehara, Umme Salma Zohora, Toshiya Osada and Atsushi Ikai.
  • Organizer: The 6th European Biophysics Congress 2007
  • Place of Presentation: London, UK.
• [Presentation] Exploring Promising Applications of Atomic Force Microscope as a Bionanotechnological Tool (2007)
  • Author(s): Afrin R., and Ikai, A.
  • Organizer: International Scanning Probe Microscopy Conference (ISPM)
  • Place of Presentation: Jeju, SOUTH KOREA
• [Presentation] Nano-mechanics of Single Protein Using Atomic Force Microscopy (2007)
  • Author(s): H. Sekiguchi, Y. Mori, and A. Ikai
  • Organizer: 21st Annual Symposium of The Protein Society
  • Place of Presentation: Boston, USA
• [Presentation] 赤血球における細胞骨格の力学的研究 (2007)
  • Author(s): 中路 雅登, 関口 博史, Afrin Rehana, 猪飼 篤
  • Organizer: 日本生物物理学会 第45回年会
  • Place of Presentation: 横浜 日本
• [Presentation] AFMによるフェリチン分子の物性計測 (2007)
  • Author(s): 関口 博史, 森 泰啓, 猪飼 篤
  • Organizer: 日本生物物理学会 第45回年会
  • Place of Presentation: 横浜 日本
• [Presentation] 電気泳動法とAFM での力学測定によるタンパク質の高感度検出の試み (2007)
  • Author(s): 日高 敦, 志賀 嘉仁, 関口 博史, 猪飼 篤, 長田 俊哉
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜 日本
• [Presentation] ウシ新規化学受容体TAARの解析 (2007)
  • Author(s): 野中 武, 中川 智貴, 若林 嘉浩, 大迫 俊二, 市川 眞澄, 大蔵 聡, 岡村 裕昭, 猪飼 篤, 長田俊哉
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜 日本
• [Presentation] 頸管粘液に反応するウシフェロモン受容体解析 (2007)
  • Author(s): 日高 翔, 流石 慎太郎, 若林 嘉浩, 大迫 俊二, 市川 眞澄, 大蔵 聡, 岡村 裕昭, 猪飼 篤, 長田 俊哉
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜 日本
• [Presentation] フェリチン分子の圧縮計測:実験とシミュレーション (2007)
  • Author(s): 関口 博史, 田上 勝規, 森 泰啓, 塚田 捷, 猪飼 篤
  • Organizer: 平成19年度日本顕微鏡学会SPM研究部会第10回研究会
  • Place of Presentation: 湯沢 日本
• [Presentation] AFM analysis of interaction forces between bio-molecules using ligand-functionalized polymers (2007)
  • Author(s): 関口 博史, 猪飼 篤, 荒川 秀雄, 杉山 滋
  • Organizer: 第27回表面科学講演大会
  • Place of Presentation: 東京 日本
• [Presentation] 赤血球における細胞骨格の弾性研究 (2007)
  • Author(s): 中路 雅登, 関口 博史, レハナ アフリン, 猪飼 篤
  • Organizer: 第27回表面科学講演大会
  • Place of Presentation: 東京 日本
• [Presentation] AFMによるフェリチン分子の押し込み力学測定 (2007)
  • Author(s): 関口 博史, 森 泰啓, 猪飼 篤
  • Organizer: 第27回表面科学講演大会
  • Place of Presentation: 東京 日本
• [Presentation] Fracture mechanics of proteins and protein based structures (2007)
  • Author(s): Atsushi Ikai
  • Organizer: The 9th International Conference on Atomically Controlled Surfaces and Nanostructures
  • Place of Presentation: Tokyo Japan
• [Book] Eds. Anne-Sophie Duwez, Nicolas WilletMicroscopy (2012)
  • Author(s): Ikai, A., Afrin, R., Watanabe-Nakayama, T. and Machida, S
  • Total Pages: 287
  • Publisher: CRC Press、Molecular Manipulation with Atomic Force Microscopy
• [Book] Ed. Yves Dufrene (2012)
  • Author(s): Ikai, A., Afrin, R., Watanabe-Nakayama, T. and Machida, S
  • Total Pages: 460
  • Publisher: Pan Stanford Publishing、Life at the Nanoscale : Atomic Force Microscopy of Live Cells
• [Book] Eds. Arturo M. Baro, Ronald G. Reifenberger (2012)
  • Author(s): Ikai, A., Afrin, R., Watanabe-Nakayama, T., Machida, S. and Saito, M
  • Total Pages: 402
  • Publisher: Wiley-VCH Atomic Force Microscopy in Liquid
• [Book] Molecular Manipulation with Atomic Force Microscopy(Eds.Anne-Sophie Duwez, Nicolas Willet) (2012)
  • Author(s): Ikai, A., Afrin, R., Watanabe-Nakayama, T., Machida, S.
  • Total Pages: 287
  • Publisher: CRC Press
• [Book] Life at the Nanoscale : Atomic Force Microscopy of Live Cells(Ed.Yves Dufrene) (2012)
  • Author(s): Ikai, A., Afrin, R., Watanabe-Nakayama, T., Machida, S.
  • Total Pages: 460
  • Publisher: Pan Stanford Publishing
• [Book] Atomic Force Microscopy in Liiquid(Eds.Arturo M.Baro, Ronald G.Reifenberger) (2012)
  • Author(s): Ikai, A., Afrin, R., Watanabe-Nakayama, T., Machida, S., Saito, M.
  • Total Pages: 402
  • Publisher: Wiley-VCH
• [Book] Advances in Chemical Physics : Single Molecule Biophysics : Experiments and Theory(Eds : T. Komatsuzaki et al.) (2011)
  • Author(s): Atsushi Ikai Rehana Afrin Hiroshi Sekiguchi
  • Total Pages: 512
  • Publisher: Force to Unbind Ligand : Receptor Complexes and the Internal Rigidity of Globular Proteins Probed by Single-Molecule Force Spectroscopy
• [Book] Life at the Nanoscale: Atomic Force Microscopy of Live Cells(Ed. Y. Dufrene, Mapping Membrane Proteins on Living Cells using Atomic Force Microscope) (2010)
  • Author(s): Atsushi Ikai, Rehana Afrin
  • Publisher: Pan Stanford Publishing Pte. Ltd.(印刷中)
• [Book] Advances in biochemical engineering/biotechnology : Nano/Micro Biotechnology (2009)
  • Author(s): Atsushi Ikai
  • Total Pages: 270
  • Publisher: A Review on : Atomic Force Microscopy Applied to Nano-mechanics of the Cell
• [Book] Elsevierドイツ語版: Einfuhrung in Die Nanobiomechanik : Bildgebung Und Messung Durch Rasterkraftmikroskopie(German Edition) by Atsushi Ikai and Carsten Heinisch(Wiley-VCH Verlag GmbH) (2008)
  • Author(s): Atsushi Ikai
  • Publisher: The World of Nano-Biomechanics : Mechanical Imaging and Measurement by Atomic Force Microscopy
• [Book] The World of Nano-Biomechanics (2008)
  • Author(s): Atsushi Ikai, Elsevier
  • Total Pages: 283
• [Book] タンパク質の事典 (2008)
  • Author(s): 猪飼篤(編集委員長)
  • Total Pages: 863
  • Publisher: 朝倉書店
• [Book] Applied Scanning Probe Method IX (B. Bhushan ed.) (2008)
  • Author(s): Kim, H., Uehara, H., Afrin, R., Sekiguchi, H., Arakawa, H., Osada T and Ikai A.
  • Total Pages: 387
  • Publisher: Springer
• [Book] The World of Nano-Biomechanics (2007)
  • Author(s): Atsushi Ikai, Rehana Afrin
  • Total Pages: 283
  • Publisher: Elsevier
• [Book] フェロモン受容に関わる神経系 (2007)
  • Author(s): 長田俊哉、市川眞澄、猪飼篤 編
  • Total Pages: 300
  • Publisher: 森北出版
• [Book] ナノテクのための工学入門 (2007)
  • Author(s): 猪飼 篤 白石賢二 編集
  • Total Pages: 243
  • Publisher: 共立出版
• [Book] ナノテクのためのパイオ入門 (2007)
  • Author(s): 猪飼 篤(共著)
  • Total Pages: 206
  • Publisher: 共立出版
• [Book] Roadmap of Scanning Probe Microscopy (S. Morita ed.) (2007)
  • Author(s): Atsushi Ikai, Rehana Afrin(共著)
  • Total Pages: 201
  • Publisher: Springer
• [Book] 細胞 (2007)
  • Author(s): 猪飼 篤、川喜田正夫、星元紀編集
  • Total Pages: 152
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.ikai.bio.titech.ac.jp/
• [Remarks]
  • URL: http://www.ikai.bio.titech.ac.jp/index.php?FrontPage