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Exploration of the limits and adaptations of life in subseafloor biosphere

Research Project

Project/Area Number 19H00730
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 17:Earth and planetary science and related fields
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

MORONO Yuki  国立研究開発法人海洋研究開発機構, 超先鋭研究開発部門(高知コア研究所), 主任研究員 (30421845)

Co-Investigator(Kenkyū-buntansha) 伊藤 民武  国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (00351742)
伊藤 元雄  国立研究開発法人海洋研究開発機構, 超先鋭研究開発部門(高知コア研究所), 調査役 (40606109)
井尻 暁  神戸大学, 海事科学研究科, 准教授 (70374212)
阪口 昌彦  大阪電気通信大学, 情報通信工学部, 准教授 (70749001)
星野 辰彦  国立研究開発法人海洋研究開発機構, 超先鋭研究開発部門(高知コア研究所), 主任研究員 (30386619)
山村 雅幸  東京工業大学, 情報理工学院, 教授 (00220442)
若松 泰介  高知大学, 教育研究部総合科学系生命環境医学部門, 准教授 (60597938)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000)
Fiscal Year 2021: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2020: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2019: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Keywords海底下生命圏 / 生命の存続限界 / 極限環境 / 生命存続限界 / 生命生息限界
Outline of Research at the Start

本研究では、生命の存続に必須の「栄養供給」と均衡する生命活動を明らかにするため、海底下地層環境を題材として、そこで連続的、経時的に変化する温度・圧力条件と生命およびその存続についての観察を実施する。この観察により、地球内部での生命圏限界、または限界をもたらす物理化学的、生理学的因子を解明し、さらに限界生命圏の姿、生命の適応メカニズム、進化学的な議論を展開することを目的としている。

Outline of Final Research Achievements

In this study, we investigated the life in subseafloor environments in the view of "nutrient supply (a critical requirement for sustaining life)" and "survival". The subseafloor sedimentary biosphere is characterized by gradual changes of parameters such as temperature, pressure, etc. In the deeper zones, both high temperature and pressure conditions restrict the survival of microbes. We investigated the profiles of microbial abundance and surrounding physicochemical parameters and found that even in extremely nutrient-poor environments, microbes can subsist and adapt to the surrounding environments.

Academic Significance and Societal Importance of the Research Achievements

生命圏の限界に近づく環境に生息する生命について研究を行うことで、生命とは何か、存在形態が地上世界で想像可能なレベルを遥かに超えて多様であることを示すことが出来た。
生命の存続に限界をもたらしうる物理化学的、生理学的因子についての知見も蓄積してきており、今後生命の適応メカニズム、進化学的な議論を展開することを期待している。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Comments on the Screening Results   Annual Research Report
  • Research Products

    (33 results)

All 2023 2021 2020 Other

All Int'l Joint Research (14 results) Journal Article (9 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 9 results,  Open Access: 7 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 4 results) Remarks (1 results)

  • [Int'l Joint Research] North Carolina University Chapel Hill/Woodshole Oceanographic Institution/Harvard University(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] GFZ German Research Centre(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of Rhode Island/Woods Hole Oceanographic Institution/University of North Carolina(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Bremen/GEOMAR/GFZ(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Ocean University of China/Hohai University/The Second Institute of Oceanography(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University Bourgogne/ULCO/IFREMER(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] CICESE/UABC/UNAM(メキシコ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Rhode Island/Woods Hole Oceanographic Institution/University of North Carolina(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Bremen/GEOMAR/GFZ(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Ocean University of China/Hohai University/The Second Institute of Oceanography(中国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University Bourgogne/ULCO/IFREMER(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] CICESE/UABC/UNAM(メキシコ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2019 Annual Research Report
  • [Journal Article] A simple method for taxon-specific purification of diatom frustules from ocean sediments using a cell sorter2023

    • Author(s)
      Kato Yuji、Morono Yuki、Ijiri Akira、Terada Takeshi、Ikehara Minoru
    • Journal Title

      Progress in Earth and Planetary Science

      Volume: 10 Issue: 1 Pages: 13-13

    • DOI

      10.1186/s40645-023-00543-5

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Terpene Synthase Gene Amplicons from Subseafloor Sediment2023

    • Author(s)
      Schmidt Ruth L.、Hoshino Tatsuhiko、Morono Yuki、Tremblay Julien、Ulanova Dana
    • Journal Title

      Microbiology Resource Announcements

      Volume: 12 Issue: 2

    • DOI

      10.1128/mra.01037-22

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Label-Free Identification of Spore-Forming Bacteria Using Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy2023

    • Author(s)
      Tanaka, K., R. Oketani, T. Terada, P. Leproux, Y. Morono and H. Kano
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 127 Issue: 9 Pages: 1940-1946

    • DOI

      10.1021/acs.jpcb.2c07291

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Modelling the Shimokita deep coalbed biosphere over deep geological time: Starvation, stimulation, material balance and population models2020

    • Author(s)
      Bowden, S. A., A. Y. Mohamed, A. N. F. Edilbi, Y. S. Lin, Y. Morono, K. U. Hinrichs and F. Inagaki
    • Journal Title

      Basin Research

      Volume: 32 Issue: 5 Pages: 804-829

    • DOI

      10.1111/bre.12399

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Temperature limits to deep subseafloor life in the Nankai Trough subduction zone2020

    • Author(s)
      Heuer Verena B.、Inagaki Fumio、Morono Yuki、Kubo Yusuke、Spivack Arthur J.、Viehweger Bernhard、Treude Tina、Beulig Felix、Schubotz Florence、Tonai Satoshi、Bowden Stephen A.、Cramm Margaret、Henkel Susann、Hirose Takehiro、Homola Kira、Hoshino Tatsuhiko、Ijiri Akira、Imachi Hiroyuki、Kamiya Nana、et al.
    • Journal Title

      Science

      Volume: 370 Issue: 6521 Pages: 1230-1234

    • DOI

      10.1126/science.abd7934

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Global diversity of microbial communities in marine sediment2020

    • Author(s)
      Hoshino Tatsuhiko、Doi Hideyuki、Uramoto Go-Ichiro、Wormer Lars、Adhikari Rishi R.、Xiao Nan、Morono Yuki、D’Hondt Steven、Hinrichs Kai-Uwe、Inagaki Fumio
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 44 Pages: 27587-27597

    • DOI

      10.1073/pnas.1919139117

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Aerobic microbial life persists in oxic marine sediment as old as 101.5 million years2020

    • Author(s)
      Morono Yuki、Ito Motoo、Hoshino Tatsuhiko、Terada Takeshi、Hori Tomoyuki、Ikehara Minoru、D’Hondt Steven、Inagaki Fumio
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 3626-3626

    • DOI

      10.1038/s41467-020-17330-1

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] EDTA-FISH: A Simple and Effective Approach to Reduce Non-specific Adsorption of Probes in Fluorescence <i>in situ</i> Hybridization (FISH) for Environmental Samples2020

    • Author(s)
      Morono, Y., K. Kubota, D. Tsukagoshi and T. Terada
    • Journal Title

      Microbes and Environments

      Volume: 35 Issue: 3 Pages: 1-1

    • DOI

      10.1264/jsme2.me20062

    • NAID

      130007865781

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Deep microbial proliferation at the basalt interface in 33.5-104 million-year-old oceanic crust2020

    • Author(s)
      Suzuki, Y., S. Yamashita, M. Kouduka, Y. Ao, H. Mukai, S. Mitsunobu, H. Kagi, S. D'Hondt, F. Inagaki, Y. Morono, T. Hoshino, N. Tomioka and M. Ito
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1 Pages: 9-9

    • DOI

      10.1038/s42003-020-0860-1

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 海底下の地層は微生物生命の楽園か、それとも永遠の牢獄なのか?2021

    • Author(s)
      諸野祐樹
    • Organizer
      マリンバイオテクノロジー学会シンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Discovery of living microbes from the ancient subseafloor sediment up to 101.5 million years old: How is it possible for microbes to survive for such a geological timescale?2021

    • Author(s)
      Yuki Morono
    • Organizer
      日本地球惑星連合 2021年大会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 白亜紀の超貧栄養海底下堆積物から生きて発見された生命2021

    • Author(s)
      Morono Y, Ito M, Hoshino T, Terada T, Hori T, Ikehara M, D'Hondt S, Inagaki F.
    • Organizer
      農芸化学会オンラインシンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 白亜紀の超貧栄養海底下堆積物から生きて発見された生命2021

    • Author(s)
      Morono Y, Ito M, Hoshino T, Terada T, Hori T, Ikehara M, D'Hondt S, Inagaki F.
    • Organizer
      日本農芸化学会2021年度仙台大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] EDTA-FISH:環境試料のFluorescence in situ Hybridization (FISH)において非特異的プローブ吸着を抑制する効果的かつシンプルなアプローチ2020

    • Author(s)
      諸野祐樹、久保田健吾、塚越大介、寺田武志
    • Organizer
      第21回極限環境生物学会年会
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Presentation] IODP Expedition 385: Guaymas Basin Tectonics and Biosphere2020

    • Author(s)
      Yuki Morono, Andreas Teske, Daniel Lizarralde, Tobias W Hofig, IODP EXPEDITION 385 SCIENTISTS
    • Organizer
      JpGU-AGU Joint Meeting 2020: Virtual
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Aerobic microbial life in oxic sediment of South Pacific Gyre persists up to 101.5 million years2020

    • Author(s)
      Morono Y, Ito M, Hoshino T, Terada T, Hori T, Ikehara M, D'Hondt S, Inagaki F.
    • Organizer
      AGU Fall meeting 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] IODP Expedition 385: Guaymas Basin Tectonics and Biosphere2020

    • Author(s)
      Yuki Morono, Andreas Teske, Daniel Lizarralde, Tobias W H?fig, IODP EXPEDITION 385 SCIENTISTS
    • Organizer
      JpGU-AGU Joint Meeting 2020
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Aerobic microbial life in oxic sediment of South Pacific Gyre persists up to 101.5 million years2020

    • Author(s)
      Morono Y, Ito M, Hoshino T, Terada T, Hori T, Ikehara M, D'Hondt S, Inagaki F.
    • Organizer
      American Geophysical Union Fall meeting 2020
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 白亜紀の海底堆積物で微生物が生きて存在していることを発見 ~超貧栄養環境下で眠り続けた生命?~

    • URL

      https://www.jamstec.go.jp/j/about/press_release/20200729/

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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