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Research on Recovery Behavior of Red Blood Cell with a Hundredfold Drastic Change

Research Project

Project/Area Number 19H00749
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 20:Mechanical dynamics, robotics, and related fields
Research InstitutionMeijo University

Principal Investigator

Kaneko Makoto  名城大学, 理工学部, 教授 (70224607)

Co-Investigator(Kenkyū-buntansha) 坂田 泰史  大阪大学, 大学院医学系研究科, 教授 (00397671)
伊藤 弘明  千葉大学, 大学院理学研究院, 助教 (10783186)
大谷 朋仁  大阪大学, 大学院医学系研究科, 講師 (30623897)
丸山 央峰  名古屋大学, 工学研究科, 准教授 (60377843)
高山 俊男  東京工業大学, 工学院, 准教授 (80376954)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000)
Fiscal Year 2022: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2020: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2019: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Keywords赤血球変形能 / ローディング / 回復時定数 / 細胞骨格 / 循環器系疾患 / 赤血球操作
Outline of Research at the Start

直径6~8μmの赤血球が血管中で一番動きにくくなるのは直径5μm以下の毛細血管を通過するときである。申請者らが開発した人工毛細血管を内蔵した高速高分解能細胞操作システムを用いて、生体内では起こり得ない狭窄部で単一赤血球を静止させたところ、静止時間3分で赤血球回復時定数が100倍激変する現象を発見した。赤血球の変形特性は赤血球内部の細胞骨格の一部であるスペクトリンやアクチンとエネルギー源ATP(アデノシン三リン酸)が支配している。本研究では人工毛細血管内狭窄部ローディング中、細胞骨格やATPが時間とともにどのように変化しているのかを調べれることにより、赤血球回復時定数激変現象を解明する。

Outline of Final Research Achievements

We discovered an interesting phenomenon on red blood cell under an artificial capillary including partially narrow section, where a red blood cell is stopped in a narrow capillary section until it is released after three minutes loading, for example. We found that the recovery time constant depends upon how much loading time is given. For example, the recovery time constant becomes 100 times more than that after the loading with just several seconds. An interesting observation is that each red blood cell results in 90% recovery of the original size, eventually, which means that the surface of each cell is not damaged, while mechanical impedance such as spring and damper are heavily changed after a long time loading.

Academic Significance and Societal Importance of the Research Achievements

赤血球の直径は高々10μmであるのに対し、毛細血管の直径は5μmである。したがって赤血球が大動脈内を流れる際には赤血球の抵抗が流れを妨害することはなく心臓に負担をかけることはない。一方赤血球が毛細血管を通過するときには毛細血管の方が細いため、通過抵抗が大きくなり、毛細血管の上流側の圧力が高くなり、結果的に心臓に負担がかかる場合がある。赤血球の変形能が低下すると、心臓への負担は益々大きくなる。糖尿病になると赤血球変形能が低くなり、ひいては心臓疾患に繋がる。ここに赤血球変形能を調べる研究は学術的意義及び社会的意義がある。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Comments on the Screening Results   Annual Research Report
  • Research Products

    (31 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (7 results) Journal Article (11 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 11 results,  Open Access: 11 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results) Remarks (3 results) Funded Workshop (1 results)

  • [Int'l Joint Research] POSTECH/0/0(韓国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] National Yang Ming Chiao Tung University/0/0(その他の国・地域(台湾))

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] POSTECH/0/0(韓国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] National Yang Ming Chiao Tung University/0/0(その他の国・地域(台湾))

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] POSTECH(韓国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Yang Ming Chiao Tung University(台湾)(その他の国・地域)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Taiwan National Chiao Tung University(その他の国・地域)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Pulsation Reduction Using Dual Sidewall-Driven Micropumps2022

    • Author(s)
      Atsumi Takuto、Takayama Toshio、Kaneko Makoto
    • Journal Title

      Micromachines

      Volume: 14 Issue: 1 Pages: 19-19

    • DOI

      10.3390/mi14010019

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Modeling and Analysis of a High-Speed Adjustable Grasping Robot Controlled by a Pneumatic Actuator2022

    • Author(s)
      Ohara Kenichi、Iwazawa Ryosuke、Kaneko Makoto
    • Journal Title

      Robotics

      Volume: 11 Issue: 1 Pages: 1-16

    • DOI

      10.3390/robotics11010027

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fast and Fine Manipulation of RBCs in Artificial Capillary and Their Mysterious Behaviors2022

    • Author(s)
      Kaneko Makoto、Tsai Chia-Hung Dylan
    • Journal Title

      Robotics Research. ISRR 2019

      Volume: 20 Pages: 102-113

    • DOI

      10.1007/978-3-030-95459-8_7

    • ISBN
      9783030954581, 9783030954598
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fabrication of Microparticles with Front?Back Asymmetric Shapes Using Anisotropic Gelation2021

    • Author(s)
      Lee Dongkyu、Kitahata Hiroyuki、Ito Hiroaki
    • Journal Title

      Micromachines

      Volume: 12 Issue: 9 Pages: 1121-1121

    • DOI

      10.3390/mi12091121

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Multilayered human skeletal muscle myoblast sheets promote the healing process after colonic anastomosis in rats2021

    • Author(s)
      Nakamura T, Yokoyama U, Kanaya T, Ueno T, Yoda T, Ishibe A, Hidaka Y. Umemura M, Takayama T, Kaneko M, Miyagawa S, Sawa Y, Endo I, Ishikawa Y.
    • Journal Title

      Cell Transplantation

      Volume: 30 Pages: 0-0

    • DOI

      10.1177/09636897211009559

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Scaffold-free tissue-engineered arterial grafts derived from human skeletal myoblasts.2021

    • Author(s)
      Saito J, Yokoyama U, Nakamura T, Kanaya T, Ueno T, Naito Y, Takayama T, Kaneko M, Miyagawa S, Sawa Y, and Ishikawa Y
    • Journal Title

      Artif Organs

      Volume: - Issue: 8 Pages: 919-932

    • DOI

      10.1111/aor.13930

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] On-Chip Micro Mixer Driven by Elastic Wall with Virtual Actuator2021

    • Author(s)
      Takayama Toshio、Kaneko Makoto、Tsai Chia-Hung Dylan
    • Journal Title

      Micromachines

      Volume: 12 Issue: 2 Pages: 217-217

    • DOI

      10.3390/mi12020217

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Challenges and Possibilities of Cell-Based Tissue-Engineered Vascular Grafts2021

    • Author(s)
      Saito Junichi、Kaneko Makoto、Ishikawa Yoshihiro、Yokoyama Utako
    • Journal Title

      Cyborg and Bionic Systems

      Volume: 2021 Pages: 1-16

    • DOI

      10.34133/2021/1532103

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rotational manipulation of a microscopic object inside a microfluidic channel2020

    • Author(s)
      Harada Hiroyuki、Kaneko Makoto、Ito Hiroaki
    • Journal Title

      Biomicrofluidics

      Volume: 14 Issue: 5 Pages: 1-16

    • DOI

      10.1063/5.0013309

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Push/Pull Inequality Based High-Speed On-Chip Mixer Enhanced by Wettability2020

    • Author(s)
      Takayama Toshio、Hosokawa Naoya、Tsai Chia-Hung Dylan、Kaneko Makoto
    • Journal Title

      Micromachines

      Volume: 11 Issue: 10 Pages: 950-950

    • DOI

      10.3390/mi11100950

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] On-chip cell manipulation and applications to deformability measurements2020

    • Author(s)
      Ito Hiroaki, Kaneko Makoto
    • Journal Title

      ROBOMECH Journal

      Volume: 7 Issue: 1 Pages: 1-11

    • DOI

      10.1186/s40648-020-0154-x

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ACTIVE STROBE IMAGER THAT CAN MEASURE MECHANICAL IMPEDANCE BY USING INPHASE OPERATIONAL MODE2023

    • Author(s)
      Taiki Yamaguchi, Osamu Fukuda, Hideaki Ito, Kensuke Harada, Koji Mizoue, and Makoto Kaneko
    • Organizer
      Tranceducers 2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of high-speed gripper with closing and releasing capability2021

    • Author(s)
      Ryosuke Iwazawa, Kenichi Ohara, and Makoto Kaneko,
    • Organizer
      The 2021 IEEE/SICE International Symposium on System Integration
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elasticity Evaluation of Red Blood Cell without Force Sensor under Large Deformation2019

    • Author(s)
      Makoto Kaneko and Koji Mizoue
    • Organizer
      IEEE The 3rd International Conference on Cyborg and Bionic Systems
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] On-chip Deformability Measurement of Eukaryotic Cells: Comparison to Anucleate Cells2019

    • Author(s)
      Hiroaki Ito, Kohei Fujimoto, and Makoto Kaneko
    • Organizer
      The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fast and Fine Manipulation of Red Blood Cells in Artificial Capillary and their Mysterious Behaviors2019

    • Author(s)
      Makoto Kaneko and Chia-Hung Dylan Tsai
    • Organizer
      International Symposium of Robotics Research
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-fingered Robotic Hands-Toward Beyond Human2019

    • Author(s)
      Makoto Kaneko
    • Organizer
      International Symposium on Crossmodal Learning in Humans and Robots
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Fast and Fine On-Chip Manipulation2019

    • Author(s)
      Makoto Kaneko
    • Organizer
      TUM, Munich, Germany
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 人手不足に対する設備の自動化--ヒトの能力を超えたロボット--2019

    • Author(s)
      金子真
    • Organizer
      日本食品・機械研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] バイオマイクロフルイディクス未解決連想問題2019

    • Author(s)
      金子真
    • Organizer
      日本学術会議第5回理論応用力学シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] 名城大学大学院理工学研究科金子研究室へようこそ

    • URL

      https://www1.meijo-u.ac.jp/~mkaneko/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 研究紹介(-科研基盤研究A:赤血球回復時定数100倍激変現象の解明を軸に-)に入り、図3、図6参照

    • URL

      https://www1.meijo-u.ac.jp/~mkaneko/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 名城大学大学院理工学研究科金子研究室へようこそ

    • URL

      http://www1.meijo-u.ac.jp/~mkaneko/

    • Related Report
      2021 Annual Research Report
  • [Funded Workshop] Research Meeting on Red Blood Cell Deformability2019

    • Related Report
      2019 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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