Tip-enhanced Nano-Raman measurement techniques for biological applications

• Project/Area Number: 19H00870
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Medium-sized Section 30:Applied physics and engineering and related fields
• Research Institution: Osaka University
• Principal Investigator: Verma Prabhat 大阪大学, 大学院工学研究科, 教授 (60362662)
• Co-Investigator(Kenkyū-buntansha): 馬越 貴之 大阪大学, 大学院工学研究科, 講師 (00793192)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥46,020,000 (Direct Cost: ¥35,400,000、Indirect Cost: ¥10,620,000); Fiscal Year 2022: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2021: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2020: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000); Fiscal Year 2019: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
• Keywords: 先端増強ラマン顕微鏡 / 近接場光学 / プラズモニクス / 超解像光学顕微鏡 / 先端増強ラマン散乱顕微鏡 / バイオイメージング

Outline of Research at the Start

生きた生体試料のありのままの姿を非染色かつナノスケールの空間分解能で観察できる技術は、ライフサイエンスに多くの新たな知見をもたらし得る。先端増強ラマン散乱顕微鏡は、これらの要件を満たし得る強力な顕微法であるが、これまで静的な材料等の観察に応用が限られていた。本研究では、先端増強ラマン顕微鏡を生体試料観察に特化することによって、生命科学を観察する新たな基盤技術を生み出す。

Outline of Final Research Achievements

This research aims to achieve operation in a liquid environment and nanoscale stability using Tip-Enhanced Raman Scattering (TERS) microscopy. We developed a new AFM head for the operation of the TERS system in a liquid environment, and additionally, we created two feedback systems to compensate for vertical and horizontal drifts. As a result, this enhanced system enables long-duration TERS imaging at the nanoscale, bringing new possibilities for the study of biological samples.

Academic Significance and Societal Importance of the Research Achievements

先端増強ラマン散乱顕微鏡は、回折限界を超えたナノスケールの空間分解能でラマンイメージングが可能な非常に強力な顕微法である。しかしながら、技術的な問題で、生命科学への応用例は限られていた。本研究では、液中環境下で動作する先端増強ラマン散乱顕微鏡を構築した。さらに、高い安定性を実現することによって、ラマン信号の弱い生体試料でも長時間にわたってイメージングできるようにした。生きた生体試料をナノレベルの空間分解能でラマン観察できるようにする上で非常に重要な成果であり、学術的にも社会的にも非常に大きな意義を有する。

Research Products

• [Journal Article] Raman Spectroscopic and DFT Study of COA-Cl and Its Analogues (2023)
  • Author(s): Umakoshi Takayuki、Urakami Takumi、Kidoguchi Haruki、Yang Keishi、Verma Prabhat、Sato Hirofumi、Higashi Masahiro、Tsukamoto Ikuko
  • Journal Title: The Journal of Physical Chemistry A Volume: 127 Issue: 8 Pages: 1849-1856
  • DOI: 10.1021/acs.jpca.2c08382
  • Peer Reviewed
• [Journal Article] Ultrastable tip-enhanced hyperspectral optical nanoimaging for defect analysis of large-sized WS2 layers (2022)
  • Author(s): Kato Ryo、Moriyama Toki、Umakoshi Takayuki、Yano Taka-aki、Verma Prabhat
  • Journal Title: Science Advances Volume: 8 Issue: 28
  • DOI: 10.1126/sciadv.abo4021
  • Peer Reviewed / Open Access
• [Journal Article] Tip-enhanced Raman spectroscopy with amplitude-controlled tapping-mode AFM (2022)
  • Author(s): Umakoshi Takayuki、Kawashima Koji、Moriyama Toki、Kato Ryo、Verma Prabhat
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 12776-12776
  • DOI: 10.1038/s41598-022-17170-7
  • Peer Reviewed / Open Access
• [Journal Article] Numerical characterization of optical properties of tapered plasmonic structure on a cantilever pyramidal tip for plasmon nanofocusing (2022)
  • Author(s): Yadav Ravi、Umakoshi Takayuki、Verma Prabhat
  • Journal Title: AIP Advances Volume: 12 Issue: 8 Pages: 085216-085216
  • DOI: 10.1063/5.0106066
  • Peer Reviewed / Open Access
• [Journal Article] Raman Spectroscopic Nanoimaging of Optical Fields of Metal Nanostructures with a Chemically Modified Metallic Tip (2021)
  • Author(s): Kato Ryo、Umakoshi Takayuki、Verma Prabhat
  • Journal Title: The Journal of Physical Chemistry C Volume: 125 Issue: 37 Pages: 20397-20404
  • DOI: 10.1021/acs.jpcc.1c04823
  • Peer Reviewed
• [Journal Article] Polarization Raman Imaging of Organic Monolayer Islands for Crystal Orientation Analysis (2021)
  • Author(s): Moriyama Toki、Umakoshi Takayuki、Hattori Yoshiaki、Taguchi Koki、Verma Prabhat、Kitamura Masatoshi
  • Journal Title: ACS Omega Volume: 6 Issue: 14 Pages: 9520-9527
  • DOI: 10.1021/acsomega.0c06313
  • NAID: 120007159894
  • Peer Reviewed / Open Access
• [Journal Article] Broadband Plasmon Nanofocusing: Comprehensive Study of Broadband Nanoscale Light Source (2021)
  • Author(s): Taguchi Koki、Umakoshi Takayuki、Inoue Shota、Verma Prabhat
  • Journal Title: The Journal of Physical Chemistry C Volume: 125 Issue: 11 Pages: 6378-6386
  • DOI: 10.1021/acs.jpcc.0c11541
  • Peer Reviewed
• [Journal Article] Probing stacking configurations in a few layered MoS2 by low frequency Raman spectroscopy (2020)
  • Author(s): Sam Rhea Thankam、Umakoshi Takayuki、Verma Prabhat
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 21227-21227
  • DOI: 10.1038/s41598-020-78238-w
  • Peer Reviewed / Open Access
• [Journal Article] Defect-related anomalous low-frequency Raman scattering in a few-layered MoS2 (2020)
  • Author(s): Sam Rhea Thankam、Umakoshi Takayuki、Verma Prabhat
  • Journal Title: Applied Physics Express Volume: 13 Issue: 7 Pages: 072003-072003
  • DOI: 10.35848/1882-0786/ab9a91
  • Peer Reviewed
• [Journal Article] One-side metal-coated pyramidal cantilever tips for highly reproducible tip-enhanced Raman spectroscopy (2020)
  • Author(s): Kato Ryo、Taguchi Koki、Yadav Ravi、Umakoshi Takayuki、Verma Prabhat
  • Journal Title: Nanotechnology Volume: 31 Issue: 33 Pages: 335207-335207
  • DOI: 10.1088/1361-6528/ab90b6
  • Peer Reviewed
• [Journal Article] White nanolight source for optical nanoimaging (2020)
  • Author(s): Umakoshi Takayuki、Tanaka Misaki、Saito Yuika、Verma Prabhat
  • Journal Title: Science Advances Volume: 6 Issue: 23
  • DOI: 10.1126/sciadv.aba4179
  • Peer Reviewed / Open Access
• [Journal Article] Tip-Enhanced Raman Spectroscopy of Multiwalled Carbon Nanotubes through D-Band Imaging: Implications for Nanoscale Analysis of Interwall Interactions (2020)
  • Author(s): Kato Ryo、Igarashi Shun、Umakoshi Takayuki、Verma Prabhat
  • Journal Title: ACS Applied Nano Materials Volume: 3 Issue: 6 Pages: 6001-6008
  • DOI: 10.1021/acsanm.0c01188
  • Peer Reviewed
• [Journal Article] Anharmonic effects in single-walled carbon nanotubes analyzed through low-temperature Raman imaging (2020)
  • Author(s): Takayuki Umakoshi, Masaki Taniguchi, Prabhat Verma
  • Journal Title: The Journal of Physical Chemistry C Volume: 124 Issue: 12 Pages: 6922-6928
  • DOI: 10.1021/acs.jpcc.0c00416
  • Peer Reviewed
• [Journal Article] Probing inter-molecular interactions of dinaphthothienothiophene (DNTT) molecules in a transistor device using low-frequency Raman spectroscopy (2020)
  • Author(s): Bishwajeet Singh Bhardwaj, Rhea Thankam Sam, Takayuki Umakoshi, Naoko Namba, Takafumi Uemura, Tsuyoshi Sekitani, Prabhat Verma
  • Journal Title: Applied Physics Express Volume: 13 Issue: 2 Pages: 22010-22010
  • DOI: 10.35848/1882-0786/ab6e0d
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Orientation analysis of pentacene molecules in organic field-effect transistor devices using polarization-dependent Raman spectroscopy (2019)
  • Author(s): Bishwajeet Singh Bhardwaj, Takeshi Sugiyama, Naoko Namba, Takayuki Umakoshi, Takafumi Uemura, Tsuyoshi Sekitani, and Prabhat Verma
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 15149-15149
  • DOI: 10.1038/s41598-019-51647-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Raman spectroscopic studies of Dinaphthothienothiophene (DNTT) (2019)
  • Author(s): Bishwajeet Singh Bhardwaj, Takeshi Sugiyama, Naoko Namba, Takayuki Umakoshi, Takafumi Uemura, Tsuyoshi Sekitani, Prabhat Verma
  • Journal Title: Materials Volume: 12 Issue: 4 Pages: 615-615
  • DOI: 10.3390/ma12040615
  • Peer Reviewed / Open Access
• [Presentation] Plasmonically Enhanced Spectroscopies for Optical Nanoimaging and Sensing (2023)
  • Author(s): Prabhat Verma
  • Organizer: 7th IEEE-EDTM Conference 2023 in Seoul
  • Int'l Joint Research / Invited
• [Presentation] White-nanolight-source for background-free nano-optical investigation (2022)
  • Author(s): Prabhat Verma
  • Organizer: MRS Fall Meeting 2022 in Boston
  • Int'l Joint Research / Invited
• [Presentation] Large-area Raman spectral nano-analysis of 2D materials by long-term TERS imaging (2021)
  • Author(s): Toki Moriyama, Ryo Kato, Takayuki Umakoshi, and Prabhat Verma
  • Organizer: JSAP-OSA joint symposia 2021
  • Int'l Joint Research
• [Presentation] Plasmon nanofocusing for near-field optical imaging (2019)
  • Author(s): Takayuki Umakoshi and Prabhat Verma
  • Organizer: Global Nanophotonics 2019
  • Int'l Joint Research / Invited
• [Presentation] Tunable Plasmonics for Sensing and Nano-Raman-Imaging (2019)
  • Author(s): Takayuki Umakoshi, Ryo Kato, Bikas Ranjan and Prabhat Verma
  • Organizer: Optics & Photonics Congress
  • Int'l Joint Research / Invited