Directed Evolution of a Palette of Optogenetic Indicators for Multiplexed Imaging of Cellular Metabolism
| Project/Area Number |
19H00920
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Medium-sized Section 37:Biomolecular chemistry and related fields
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| Research Institution | The University of Tokyo |
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Principal Investigator |
キャンベル ロバート.アール 東京大学, 大学院理学系研究科(理学部), 教授 (40831318)
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| Project Period (FY) |
2019-04-01 – 2020-03-31
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| Project Status |
Discontinued (Fiscal Year 2019)
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| Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2019: ¥29,900,000 (Direct Cost: ¥23,000,000、Indirect Cost: ¥6,900,000)
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| Keywords | Protein Engineering / Fluorescence / Microscopy / Cell Biology / Cell signalling / Metabolism |
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| Outline of Research at the Start |
Advances in microscopy and fluorescent probe technology have revolutionized medical and biological research by enabling the normally colorless world of the cell to be visualized in high resolution and with vivid colors. However, efforts to understand the mechanisms by which cellular metabolism changes in diseased states have been hampered by a lack of high-performance indicators. To better understand these mechanisms, we will use innovative directed protein evolution approaches to create indicators with large signal changes and colors ranging from the visible to the near-infrared.
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| Outline of Annual Research Achievements |
The goal of this project was to perform "Directed Evolution of a Palette of Optogenetic Indicators for Multiplexed Imaging of Cellular Metabolism" by using the Grant-in-Aid for Scientific Research (A). In this fiscal year, this grant enabled us to set up the laboratory for the study mentioned above. For example, we purchased a wide-field fluorescence microscopy, which Campbell lab is using to accelerate the development of a range of optogenetic indicators.
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| Research Progress Status |
令和元年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和元年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(26 results)
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[Journal Article] A genetically encoded near-infrared fluorescent calcium ion indicator2019
Author(s)
Y. Qian (equal contribution), K.D. Piatkevich (equal contribution), B. McLarney (equal contribution), A.S. Abdelfattah, S. Mehta, M.H. Murdock, S. Gottschalk, R.S. Molina, W. Zhang, Y. Chen, J. Wu, M. Drobizhev, T.E. Hughes, J. Zhang, E.R. Schreiter, S. Shoham, D. Razansky, E.S. Boyden, and R.E. Campbell*
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Journal Title
Nat. Methods
Volume: 16
Issue: 2
Pages: 171-174
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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