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Directed Evolution of a Palette of Optogenetic Indicators for Multiplexed Imaging of Cellular Metabolism

Research Project

Project/Area Number 19H00920
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 37:Biomolecular chemistry and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

キャンベル ロバート.アール  東京大学, 大学院理学系研究科(理学部), 教授 (40831318)

Project Period (FY) 2019-04-01 – 2020-03-31
Project Status Discontinued (Fiscal Year 2019)
Budget Amount *help
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2019: ¥29,900,000 (Direct Cost: ¥23,000,000、Indirect Cost: ¥6,900,000)
KeywordsProtein Engineering / Fluorescence / Microscopy / Cell Biology / Cell signalling / Metabolism
Outline of Research at the Start

Advances in microscopy and fluorescent probe technology have revolutionized medical and biological research by enabling the normally colorless world of the cell to be visualized in high resolution and with vivid colors. However, efforts to understand the mechanisms by which cellular metabolism changes in diseased states have been hampered by a lack of high-performance indicators. To better understand these mechanisms, we will use innovative directed protein evolution approaches to create indicators with large signal changes and colors ranging from the visible to the near-infrared.

Outline of Annual Research Achievements

The goal of this project was to perform "Directed Evolution of a Palette of Optogenetic Indicators for Multiplexed Imaging of Cellular Metabolism" by using the Grant-in-Aid for Scientific Research (A). In this fiscal year, this grant enabled us to set up the laboratory for the study mentioned above. For example, we purchased a wide-field fluorescence microscopy, which Campbell lab is using to accelerate the development of a range of optogenetic indicators.

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2019 Comments on the Screening Results   Annual Research Report
  • Research Products

    (26 results)

All 2020 2019 Other

All Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (20 results) (of which Int'l Joint Research: 16 results,  Invited: 17 results) Remarks (1 results)

  • [Journal Article] Engineering genetically encoded fluorescent indicators for optical imaging of neuroscience: progress and prospects2020

    • Author(s)
      Y. Shen, Y. Nasu, I. Shkolnikov, A. Kim and R.E. Campbell
    • Journal Title

      Neuroscience Research

      Volume: 未定

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 20 years of engineering single fluorescent protein-based biosensors2020

    • Author(s)
      Y. Nasu, Y. Shen, L. Kramer, and R.E. Campbell
    • Journal Title

      Nat. Chem. Biol.

      Volume: 未定

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genetically encoded fluorescent indicators for imaging intracellular potassium ions2019

    • Author(s)
      Y. Shen, S.-Y. Wu, V. Rancic, A. Aggarwal, Y. Qian, S.-I. Miyashita, K. Ballanyi, R.E. Campbell*, and M. Dong*
    • Journal Title

      Commun. Biol.

      Volume: 2 Issue: 1 Pages: 18-18

    • DOI

      10.1038/s42003-018-0269-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A genetically encoded near-infrared fluorescent calcium ion indicator2019

    • Author(s)
      Y. Qian (equal contribution), K.D. Piatkevich (equal contribution), B. McLarney (equal contribution), A.S. Abdelfattah, S. Mehta, M.H. Murdock, S. Gottschalk, R.S. Molina, W. Zhang, Y. Chen, J. Wu, M. Drobizhev, T.E. Hughes, J. Zhang, E.R. Schreiter, S. Shoham, D. Razansky, E.S. Boyden, and R.E. Campbell*
    • Journal Title

      Nat. Methods

      Volume: 16 Issue: 2 Pages: 171-174

    • DOI

      10.1038/s41592-018-0294-6

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators2019

    • Author(s)
      R. Molina, Y. Qian, J. Wu, Y. Shen, R.E. Campbell, T. Hughes, and M. Drobizhev
    • Journal Title

      Biophys. J.

      Volume: 116 Issue: 10 Pages: 1873-1886

    • DOI

      10.1016/j.bpj.2019.04.007

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Filling the visible spectrum, and beyond, with genetically encoded fluorescent probes of cell signalling and metabolism2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      The 10th IBRO World Congress of Neuroscience, EXCO Convention Center, Daegu, Korea
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Engineering new protein-based biosensors to illuminate signalling and metabolism in the brain2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      World Molecular Imaging Congress, “Synthetic Biology and Brain Function”, Montreal QC
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The legacy of camgaroo: new fluorescent spies of neural activity and metabolism2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Roger Tsien Memorial Symposium, La Jolla, CA
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Engineering protein-based biosensors for fluorescence imaging of cell signalling and metabolism2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Department of Chemistry, National Taiwan University, Taipei, Taiwan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Engineering protein-based biosensors for fluorescence imaging of cell signalling and metabolism2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Institute of Chemical Biology, Academia Sinica, Taipei, Taiwan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetically Encoded Tools [Fluorophores] and their Applications2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      LumiSTAR Opening Ceremony, National Biotechnology Research Park, Taipei, Taiwan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Engineering genetically-encoded fluorescent indicators for dynamic visualization of cell signaling and metabolism2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Quantitative Biology 2019: Dynamic Signaling in Cells and Embryos, Yantai, China
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Filling the visible spectrum, and beyond, with genetically encoded fluorophores and indicators2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      CCMA 2019, Cytometry & Microscopy Symposium, Edmonton, AB
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetically encoded fluorescent probes for multi-parameter imaging of cell signalling and metabolism2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Advances in Fluorescent Probe and Fluorescence Imaging Modalities, 102nd Canadian Chemistry Conference and Exhibition, Quebec City, QC
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetically encoded fluorophores and reporters to illuminate neuronal activity2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Neurophotonics Summer School, Cervo Brain Research Centre
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging of cell signalling with micron-scale genetically encoded biosensing beacons2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Serendipity Lab Kickoff Symposium (Half-Global), University of Tokyo
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] New genetically encoded biosensors for fluorescence imaging of cell signalling and metabolism2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Microscopical Society of Canada 46th Annual Meeting, Vancouver, BC
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Fluorescent probes and proteins2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Analytical & Quantitative Light Microscopy, Marine Biological Laboratory, Woods Hole, MA
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetically-encoded red and near-infrared neural activity indicators and new indicator development2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      OSA Biophotonics Congress: Optics and the Brain (New Indicators), Tucson, AZ
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multiplexed imaging with red-shifted optogenetic indicators2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      NIH Neuroscience Seminar Series, Bethesda, MD
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Protein engineering and directed evolution for the creation of optogenetic indicators2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Department of Chemistry, Purdue University
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Filling the visible spectrum, and beyond, with genetically encoded indicators of cell signalling and metabolism2019

    • Author(s)
      Robert E. Campbell
    • Organizer
      Department of Cell Biology Seminar Series
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Engineering and directed evolution of protein-based tools2019

    • Author(s)
      Yusuke Nasu, Robert E. Campbell
    • Organizer
      The 19th BIO UT
    • Related Report
      2019 Annual Research Report
  • [Presentation] Metabolismの「非」常識を検証する -近赤外蛍光乳酸センサーの開発-2019

    • Author(s)
      Yusuke Nasu, Robert E. Campbell
    • Organizer
      The 4th Toyota Riken meeting
    • Related Report
      2019 Annual Research Report
  • [Presentation] Development of a genetically encoded intensiometric lactate indicator iLACCO12019

    • Author(s)
      Yusuke Nasu, Robert E. Campbell
    • Organizer
      ACS Fall 2019 National Meeting & Exposition
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Remarks] Biomolecular Chemistry Laboratory

    • URL

      https://www.chem.s.u-tokyo.ac.jp/campbell/

    • Related Report
      2019 Annual Research Report

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Published: 2019-04-18   Modified: 2021-12-27  

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