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Dissecting role of sucrose and GA recognition in SWEET transporters

Research Project

Project/Area Number 19H00932
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 38:Agricultural chemistry and related fields
Research InstitutionNagoya University

Principal Investigator

Frommer Wolf  名古屋大学, トランスフォーマティブ生命分子研究所, 客員教授 (70795575)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2021: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2020: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2019: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
KeywordsSucrose / Gibberellin / SWEET / substrate selectivity / Phytohormone / Gibberellic acid / Sugar / SWEETs / Transporter / Gibberelllic acid
Outline of Research at the Start

Sugars and phytohormones are essential to the fundamental processes of plant development and growth. It has remained unclear, however, how SWEETs can transport substrates as different as sucrose and GA. We will focus and clarify the substrate selectivity of GA and sucrose transporting SWEETs.

Outline of Final Research Achievements

Substrate selectivity of transporters is critical to accomplish the complex systems in biological processes. However, the molecular mechanisms of substrate selectivity of transporters have not been fully elucidated. SWEETs play essential roles through the transport activity for sucrose and/or glucose. In addition to sugars, SWEETs can transport GA, a hormone that regulates many important aspects of plant growth. Sugars and phytohormones are essential to the fundamental processes of plant development and growth. It has remained unclear, however, which spectrum of substrates SWEETs can transport, and how SWEETs can transport substrates as different as sucrose and GA. In this project, we investigated the substrate selectivity of GA and sucrose transporting SWEETs using computational and biochemical approaches. We have determined the residues responsible for GA and sucrose recognition in SWEETs and whether sucrose and GA are physiologically relevant for male fertility in Arabidopsis.

Academic Significance and Societal Importance of the Research Achievements

酵素や輸送体は無数の基質と相互作用するが、その中で生理的に意義があるものはごくわずかである。SWEETの基質選択性の分子メカニズムや輸送活性の生理的意義を解析することで、輸送体や酵素の持つ複数の基質活性の理解が深まる。本研究で得られた知見は、農薬や抗菌剤の開発などの創薬分野にも役に立つと考えられる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Comments on the Screening Results   Annual Research Report
  • Research Products

    (3 results)

All 2022 2021 2020

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results)

  • [Journal Article] <i>OsSWEET11b</i>, a potential sixth leaf blight susceptibility gene involved in sugar transport‐dependent male fertility2022

    • Author(s)
      Wu L-B, Eom J-S, Isoda R, Li C, Char SN, Luo D, Schepler‐Luu V, Nakamura M, Yang B, Frommer WB
    • Journal Title

      New Phytologist

      Volume: 234 Issue: 3 Pages: 975-989

    • DOI

      10.1111/nph.18054

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Advances in Synthetic Fluorescent Probe Labeling for Live-Cell Imaging in Plants2021

    • Author(s)
      Yagi N, Yoshinari A, Iwatate RJ, Isoda R, Frommer WB, Nakamura M
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 8 Pages: 1259-1268

    • DOI

      10.1093/pcp/pcab104

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sensors for the quantification, localization and analysis of the dynamics of plant hormones2020

    • Author(s)
      Isoda Reika、Yoshinari Akira、Ishikawa Yuuma、Sadoine Mayuri、Simon Rudiger、Frommer Wolf B.、Nakamura Masayoshi
    • Journal Title

      The Plant Journal

      Volume: 105 Issue: 2 Pages: 542-557

    • DOI

      10.1111/tpj.15096

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research

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Published: 2019-04-18   Modified: 2023-01-30  

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