| Budget Amount *help |
¥45,240,000 (Direct Cost: ¥34,800,000、Indirect Cost: ¥10,440,000)
Fiscal Year 2022: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2021: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2020: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2019: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
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| Outline of Final Research Achievements |
The aim of study was understanding of the mechanisms underlying the polar localization of nutrient transporters in plant cells. We have previously shown that continuous endocytosis and exocytosis are required for the maintenance of the polar localization of a boric acid channel NIP5;1 and a borate exporter BOR1 toward the soil- and stele-side plasma membrane domains, respectively, in root cells. In this study, we further investigated the mechanisms utilizing genetics and proteomics approaches. As a major finding, we identified the importance of both phosphorylation and dephosphorylation of BOR1 C-terminal region in the regulation of the polar trafficking.
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